Forensic DNA Exam – DNA Separation, Electrophoresis Techniques & Capillary Detection Methods|Complete Verified Questions Provided with A+ Graded Rationales Latest Updated 2026

Forensic DNA Exam – DNA Separation, Electrophoresis Techniques & Capillary Detection Methods|Complete Verified Questions Provided with A+ Graded Rationales Latest Updated 2026 Electrophoresis the separation of DNA fragments based on size when a current is applied Cathode negative electrode (beginning of electrophoresis) Anode positive electrode (end of electrophoresis) The higher the voltage applied, the ____ (slower/faster) the DNA moves. faster What are the 2 types of electrophoresis slab gels? 1. agarose 2. polyacrylamide What are the 3 purposes of a buffer in gel electrophoresis? 1. provides ions to carry current 2. maintains constant pH 3. dissipates heat produced by current Agarose gels provide high resolution for ____ (small/large) DNA fragments. large (ex. RFLPs) Agarose gels have ____ (small/large) pore sizes large (allows for larger DNA fragments to be separated) The higher the agarose concentration, the ____ (lower/higher) resolution will be produced. higher (tighter bands) Polyacrylamide gels provide high resolution for ____ (small/large) DNA fragments. small (ex. STRs) Polyacrylamide gels have ____ (small/large) pore sizes small (can differentiate microvariants) The higher the polyacrylamide concentration, the ____ (lower/higher) resolution will be produced. higher Native system double-stranded DNA fragments being separated during electrophoresis Denaturing system single-stranded DNA fragments being separated during electrophoresis Is native or denaturing system preferred for electrophoresis? denaturing Why is denaturing system preferred? 1. provides better