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QIHC 2026 QUESTIONS AND
ANSWERS| ACE YOUR GRADES.
1. Part of the antibody that binds to the epitope of the antigen -
correct answer -Paratope
2. This region determines the antibody class - correct answer -Fc
region "tail of the Y"
3. When performing multiple stains using secondary antibodies
from different species, it may be necessary to use - correct
answer -Blocking sera from the species of both secondary
antibodies
4. This is a common blocking agent as it contains antibodies that
bind to non specific sites. Using it with the same species as
secondary antibody is recommended - correct answer -Serum
5. When performing multiple stains using secondary antibodies
from different species it may be necessary to use this - correct
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answer -Blocking sera from species of both secondary
antibodies
6. This is a dye that absorbs light and then emits it's own light at a
longer wavelength - correct answer -Fluorophore/fluorochrome
7. For this type of amp using labeled tyramide or Nanogold-silver
staining is an important signal amplification tool - correct
answer -Catalyzed signal amplification (CSA)
8. What is a step to use in IHC/ISH methods that enhance the
sensitivity - correct answer -Signal amplification
9. What is used for tyramide signal amplification (TSA) for
increasing sensitivity in multicolor ICC, IHC, and ISH - correct
answer -Fluorescein tyramide
10. Other heavy metals that amplify signal - correct answer -
Gold, Silver, nickel and cobalt ...sodium azide?
QIHC 2026 QUESTIONS AND
ANSWERS| ACE YOUR GRADES.
1. Part of the antibody that binds to the epitope of the antigen -
correct answer -Paratope
2. This region determines the antibody class - correct answer -Fc
region "tail of the Y"
3. When performing multiple stains using secondary antibodies
from different species, it may be necessary to use - correct
answer -Blocking sera from the species of both secondary
antibodies
4. This is a common blocking agent as it contains antibodies that
bind to non specific sites. Using it with the same species as
secondary antibody is recommended - correct answer -Serum
5. When performing multiple stains using secondary antibodies
from different species it may be necessary to use this - correct
, Page | 2
answer -Blocking sera from species of both secondary
antibodies
6. This is a dye that absorbs light and then emits it's own light at a
longer wavelength - correct answer -Fluorophore/fluorochrome
7. For this type of amp using labeled tyramide or Nanogold-silver
staining is an important signal amplification tool - correct
answer -Catalyzed signal amplification (CSA)
8. What is a step to use in IHC/ISH methods that enhance the
sensitivity - correct answer -Signal amplification
9. What is used for tyramide signal amplification (TSA) for
increasing sensitivity in multicolor ICC, IHC, and ISH - correct
answer -Fluorescein tyramide
10. Other heavy metals that amplify signal - correct answer -
Gold, Silver, nickel and cobalt ...sodium azide?
