Page | 1
QIHC STUDY GUIDE – 5 2026
QUESTIONS AND ANSWERS| ACE
YOUR GRADES.
Araldite or LR White - correct answer -Example of resin infiltrated
on tissues
Sectioning - correct answer -The production of thin slices of the
specimen
So that they are semitransparent to electrons - correct answer -
Why must the tissues be very thin during sectioning?
90nm - correct answer -Tissue sections are typically in what size?
Glass or diamond knife - correct answer -Knives used for cutting
tissues
, Page | 2
True - correct answer -T/F. Glass knives blunt very quickly and
therefore need replacing frequently
Lead and Uranium - correct answer -Heavy metals used to scatter
imaging electrons
Freeze-fracture and freeze-etch - correct answer -A preparation
method particularly useful for examining lipid membranes and
their incorporated proteins in "face on" view.
evaporated platinum and carbon - correct answer -What is used in
freeze-fracture and freeze-etch to improve stability of the replica
coating?
Sputter coating - correct answer -An ultra-thin coating of
electrically-conducting material, deposited by low vacuum coating
of the sample
They occur because of the accumulation of static electric fields -
correct answer -Why do charging of the specimen occur?
, Page | 3
Due to the electron irradiation required during imaging - correct
answer -What is the cause of the accumulation of static electric
fields?
Gold, gold/palladium, platinum, chromium - correct answer -
Coatings used in sputter coating
No staining/weak staining
High background staining
Dual staining
mouse on mouse staining system - correct answer -Challenges in
IHC
Dual staining - correct answer -Refers to staining antigens
simultaneously to increase antigen expression for studies and
observation
Mouse on Mouse Staining System - correct answer -Refers to
using mouse models in studies and observation, specially mouse
monoclonals for IHC staining
QIHC STUDY GUIDE – 5 2026
QUESTIONS AND ANSWERS| ACE
YOUR GRADES.
Araldite or LR White - correct answer -Example of resin infiltrated
on tissues
Sectioning - correct answer -The production of thin slices of the
specimen
So that they are semitransparent to electrons - correct answer -
Why must the tissues be very thin during sectioning?
90nm - correct answer -Tissue sections are typically in what size?
Glass or diamond knife - correct answer -Knives used for cutting
tissues
, Page | 2
True - correct answer -T/F. Glass knives blunt very quickly and
therefore need replacing frequently
Lead and Uranium - correct answer -Heavy metals used to scatter
imaging electrons
Freeze-fracture and freeze-etch - correct answer -A preparation
method particularly useful for examining lipid membranes and
their incorporated proteins in "face on" view.
evaporated platinum and carbon - correct answer -What is used in
freeze-fracture and freeze-etch to improve stability of the replica
coating?
Sputter coating - correct answer -An ultra-thin coating of
electrically-conducting material, deposited by low vacuum coating
of the sample
They occur because of the accumulation of static electric fields -
correct answer -Why do charging of the specimen occur?
, Page | 3
Due to the electron irradiation required during imaging - correct
answer -What is the cause of the accumulation of static electric
fields?
Gold, gold/palladium, platinum, chromium - correct answer -
Coatings used in sputter coating
No staining/weak staining
High background staining
Dual staining
mouse on mouse staining system - correct answer -Challenges in
IHC
Dual staining - correct answer -Refers to staining antigens
simultaneously to increase antigen expression for studies and
observation
Mouse on Mouse Staining System - correct answer -Refers to
using mouse models in studies and observation, specially mouse
monoclonals for IHC staining
