BIOCHEM ACS FINAL EXAM REVIEW 2026 FULL STUDY GUIDE WITH VERIFIED Q&A

BIOCHEM ACS FINAL EXAM REVIEW 2026
FULL STUDY GUIDE WITH VERIFIED Q&A

⩥ pka of acetic acid. Answer: 4.76


⩥ pka of H3PO4. Answer: 2, 7, 12


⩥ pka of the amino terminal of AA. Answer: 10


⩥ pka of the carboxyl terminal of AA. Answer: 2


⩥ what are the charged amino acids. Answer: arg
lys
his
asp
glu


⩥ what are the basic AA. Answer: arg
lys
his

,⩥ what are the acidic AA. Answer: asp
glu


⩥ pka of arg. Answer: 12


⩥ pka of lys. Answer: 10


⩥ pka of his. Answer: 6


⩥ pka of asp. Answer: 4


⩥ pka of glu. Answer: 4


⩥ if the pH is less than the pka. Answer: AA keeps proton


⩥ if the pH is greater than the pKa. Answer: AA loses proton


⩥ nonpolar AA. Answer: gly
leu
ala
met
val

, ile
pro
cys
trp
phe


⩥ polar AA. Answer: ser
thr
tyr
asn
gln


⩥ size exclusion chromatography. Answer: larger proteins elute first
separate proteins based on size


⩥ cation exchange. Answer: cationic proteins stay on column
anionic proteins elute out
increase competition by adding high salt buffer (NaCl/KCl) or increase
pH of column itself
separate protein based on positive charge