MOLECULAR DIAGNOSTICS 3RD EDITION
BUCKINGHAM TEST BANK ACTUAL EXAM
2026 FULL QUESTIONS AND CORRECT
ANSWERS GRADED A+
⩥ RNA polymerase. Answer: enzyme that links together the growing
chain of RNA nucleotides during transcription using a DNA strand as a
template, occurs during metaphase
⩥ Which RNA polymerase synthesize noncoding RNA?. Answer: Pol I
and III
⩥ Promoter. Answer: specific region of a gene where RNA polymerase
can bind and begin transcription
⩥ Are there more RNA polymerase or DNA polymerase in the cell?.
Answer: RNA polymerase
⩥ Does the antisense strand or sense strand if the DNA template have an
identical sequence to that of the RNA product?. Answer: the sense strand
has an identical sequence to the mRNA (except for T-->U sub.), the
antisense strand is used as the template
, ⩥ What are the three steps of mRNA capping?. Answer: 1. hydrolysis of
the 5' phosphate
2. transfer of a guanine monophosphate
3. methylation of the attached guanine in the N7 position
⩥ What is the purpose of the cap on mRNA?. Answer: protects the cap
during transcription and facilitates ribosome binding during translation
⩥ Rho. Answer: transcription factor that inactivates the elongation
complex at the site of termination
⩥ In eukaryotes when does transcription end?. Answer: at the
poladenylated site which activates termination of transcription
⩥ isoforms. Answer: closely related proteins whose function is similar
but whose affinity for ligands differs
⩥ In prokaryotes how does transcription end?. Answer: 1. negative
feedback of protein levels
2. Rho a transcription complex factor stops elongation of the RNA
transcript
⩥ constitutive. Answer: Proteins always present
BUCKINGHAM TEST BANK ACTUAL EXAM
2026 FULL QUESTIONS AND CORRECT
ANSWERS GRADED A+
⩥ RNA polymerase. Answer: enzyme that links together the growing
chain of RNA nucleotides during transcription using a DNA strand as a
template, occurs during metaphase
⩥ Which RNA polymerase synthesize noncoding RNA?. Answer: Pol I
and III
⩥ Promoter. Answer: specific region of a gene where RNA polymerase
can bind and begin transcription
⩥ Are there more RNA polymerase or DNA polymerase in the cell?.
Answer: RNA polymerase
⩥ Does the antisense strand or sense strand if the DNA template have an
identical sequence to that of the RNA product?. Answer: the sense strand
has an identical sequence to the mRNA (except for T-->U sub.), the
antisense strand is used as the template
, ⩥ What are the three steps of mRNA capping?. Answer: 1. hydrolysis of
the 5' phosphate
2. transfer of a guanine monophosphate
3. methylation of the attached guanine in the N7 position
⩥ What is the purpose of the cap on mRNA?. Answer: protects the cap
during transcription and facilitates ribosome binding during translation
⩥ Rho. Answer: transcription factor that inactivates the elongation
complex at the site of termination
⩥ In eukaryotes when does transcription end?. Answer: at the
poladenylated site which activates termination of transcription
⩥ isoforms. Answer: closely related proteins whose function is similar
but whose affinity for ligands differs
⩥ In prokaryotes how does transcription end?. Answer: 1. negative
feedback of protein levels
2. Rho a transcription complex factor stops elongation of the RNA
transcript
⩥ constitutive. Answer: Proteins always present
