MLT Practice Exam with Accurate
Solutions
c. 1000 - ANS-To correctly report a colony count in a urine culture plated using a
calibrated 0.001 mL loop, the observed colony count must be multiplied by which of the
following?
a. 1
b. 100
c. 1000
d. 2000
c. Failure of the lymphocyte population to begin at baseline on the leukocyte histogram -
ANS-Examination of peripheral blood smear revealed 12 nucleated erythrocytes per
100 leukocytes. Which histogram observation correlates with this finding?
a. Failure of the granulocyte population to return to baseline on the leukocyte histogram
b. Shift to the left of the erythrocyte population on the erythrocyte histogram
c. Failure of the lymphocyte population to begin at baseline on the leukocyte histogram
d. Shift to the right of the platelet population on the platelet histogram
a. Minimize the exposure to oxygen - ANS-A specimen for anaerobic culture is place in
an anaerobic holding jar in order to:
a. Minimize exposure to oxygen
b. Maximize spore formation
c. Increase the growth rate of the sample
d. Decrease the exposure to UV light
a. Phase Contrast - ANS-A clinical laboratory technician wishes to examine an
unstained, wet preparation of cells or organism prior to dehydration. Which of the
following types of microscopes would be best to use in this scenario?
a. Phase Contrast
b. Fluorescence
c. Brightfield
d. Electron
a. Proteus mirabilis - ANS-An organism gives the following reactions: TSI and KIA
alkaline slant over an acid butt, H2S positive ferments glucose. Based on these
reactions, what is the most likely identification of the organism?
a. Proteus mirabilis
b. Esherichia coli
c. Pseudomonas aeruginosa
d. Salmonella sp.
, D.No definitive conclusion can be made from this data. Further testing is necessary -
ANS-A pregnant woman who has active syphillis and a RPR of 1:32 is being treated
with penicillin. She gives birth to a baby boy. When the cord blood from the baby is
tested, the RPR is 1:2. Based on the above data, the most likely interpretation is:
a. The baby has congenital syphillis
b. The baby does not have syphillis. A titer this low represents passive acquisition of the
antibody from the mother
c. The baby does not have syphillis. A titer this low represents a biological false positive
d. No definitive conclusion can be made from this data. Further testing is necessary
d.An 18-year old male taking antibiotics for a severe case of acne - ANS-Which of the
following potential donors would be acceptable for donation of whole blood?
a. An individual who had a tattoo applied
b. A phlebotomist with a reported needle stick injury 10 months ago
c. An individual who has just immigrated to U.S. from Great Britain
d. An 18-year old male taking antibiotics for a severe case of acne
b.Dimorphic fungus present - ANS-An organism from a lung biopsy grew as mould at
room temperature and a yeast at 37C. what is the most appropriate way to interpret
these results?
a. Specimen is contaminated, ask for repeat collection
b. Dimorphic fungus present
c. Two organisms are present but only the mould is significant
d. Two pathogens are present
c.1:512 - ANS-In a serological serial dilution, if tube 1 has 1mL of an undiluted
specimen and doubling dilutions are used thereafter, what is the dilution in tube 10?
a. 1:200
b. 1:500
c. 1:512
d. 1:1024
c.Agglutination of horse red blood cells after absorption of the serum specimen by
guinea pig kidney cells - ANS-Infectious mononucleosis exhibits what serological
reaction below when serum from an actively infected patient is tested?
a. Agglutination of guinea pig kidney cells after absorption of the serum specimen with
horse red blood cells
b. Agglutination of beef red blood cells after absorption of the serum specimen with
horse red blood cells
c. Agglutination of horse red blood cells after absorption of the serum specimen by
guinea pig kidney cells
d. Agglutination of horse red blood cells after absorption of the serum specimen by beef
red blood cells
Solutions
c. 1000 - ANS-To correctly report a colony count in a urine culture plated using a
calibrated 0.001 mL loop, the observed colony count must be multiplied by which of the
following?
a. 1
b. 100
c. 1000
d. 2000
c. Failure of the lymphocyte population to begin at baseline on the leukocyte histogram -
ANS-Examination of peripheral blood smear revealed 12 nucleated erythrocytes per
100 leukocytes. Which histogram observation correlates with this finding?
a. Failure of the granulocyte population to return to baseline on the leukocyte histogram
b. Shift to the left of the erythrocyte population on the erythrocyte histogram
c. Failure of the lymphocyte population to begin at baseline on the leukocyte histogram
d. Shift to the right of the platelet population on the platelet histogram
a. Minimize the exposure to oxygen - ANS-A specimen for anaerobic culture is place in
an anaerobic holding jar in order to:
a. Minimize exposure to oxygen
b. Maximize spore formation
c. Increase the growth rate of the sample
d. Decrease the exposure to UV light
a. Phase Contrast - ANS-A clinical laboratory technician wishes to examine an
unstained, wet preparation of cells or organism prior to dehydration. Which of the
following types of microscopes would be best to use in this scenario?
a. Phase Contrast
b. Fluorescence
c. Brightfield
d. Electron
a. Proteus mirabilis - ANS-An organism gives the following reactions: TSI and KIA
alkaline slant over an acid butt, H2S positive ferments glucose. Based on these
reactions, what is the most likely identification of the organism?
a. Proteus mirabilis
b. Esherichia coli
c. Pseudomonas aeruginosa
d. Salmonella sp.
, D.No definitive conclusion can be made from this data. Further testing is necessary -
ANS-A pregnant woman who has active syphillis and a RPR of 1:32 is being treated
with penicillin. She gives birth to a baby boy. When the cord blood from the baby is
tested, the RPR is 1:2. Based on the above data, the most likely interpretation is:
a. The baby has congenital syphillis
b. The baby does not have syphillis. A titer this low represents passive acquisition of the
antibody from the mother
c. The baby does not have syphillis. A titer this low represents a biological false positive
d. No definitive conclusion can be made from this data. Further testing is necessary
d.An 18-year old male taking antibiotics for a severe case of acne - ANS-Which of the
following potential donors would be acceptable for donation of whole blood?
a. An individual who had a tattoo applied
b. A phlebotomist with a reported needle stick injury 10 months ago
c. An individual who has just immigrated to U.S. from Great Britain
d. An 18-year old male taking antibiotics for a severe case of acne
b.Dimorphic fungus present - ANS-An organism from a lung biopsy grew as mould at
room temperature and a yeast at 37C. what is the most appropriate way to interpret
these results?
a. Specimen is contaminated, ask for repeat collection
b. Dimorphic fungus present
c. Two organisms are present but only the mould is significant
d. Two pathogens are present
c.1:512 - ANS-In a serological serial dilution, if tube 1 has 1mL of an undiluted
specimen and doubling dilutions are used thereafter, what is the dilution in tube 10?
a. 1:200
b. 1:500
c. 1:512
d. 1:1024
c.Agglutination of horse red blood cells after absorption of the serum specimen by
guinea pig kidney cells - ANS-Infectious mononucleosis exhibits what serological
reaction below when serum from an actively infected patient is tested?
a. Agglutination of guinea pig kidney cells after absorption of the serum specimen with
horse red blood cells
b. Agglutination of beef red blood cells after absorption of the serum specimen with
horse red blood cells
c. Agglutination of horse red blood cells after absorption of the serum specimen by
guinea pig kidney cells
d. Agglutination of horse red blood cells after absorption of the serum specimen by beef
red blood cells
