MB (ASCP) QUESTIONS
& ANSWERS WITH COMPLETE
SOLUTIONS GRADED A+
QUESTIONS AND ANSWERS
what does topoisomerase do?. ANSWER -breaks phosphate backbone to "un"
supercoil DNA
Another name for Topoisomerase II. ANSWER -Gyrase
Helicase. ANSWER -Un"zips" DNA at replication fork
What makes up the primosome?. ANSWER -1. primase
2. helicase
3. DNA C
NASBA stands for.... ANSWER -nucleic acid sequence based amplification
NASBA. ANSWER -RNA is target
RNA-->cDNA
Reverse transcriptase (RNA-->cDNA) followed by DNA dependent polymerase
(cDNA-->RNA)
RNAse H degrades original RNA template
,Evaluated gene EXPRESSION. ANSWER -RNA
ratio for organic extraction. ANSWER -Phenol (50): Chloroform (49): isoamyl alcohol
(1)
phenol. ANSWER -denatures proteins, dissolves lipids
chloroform. ANSWER -assists in dissolving remaining lipids, gives a clear interphase
between aqueous phase
Long term storage of RNA. ANSWER -70% EtOH at -70 C
ratio for 28S to 18S subunits. ANSWER -2:1 (can see this in a gel)
A260. ANSWER -nucleic acids
A280. ANSWER -protein
A230. ANSWER -organic compounds, phenol
A270. ANSWER -phenol
, optical density of ds DNA. ANSWER -50 ug/ml
optical density ssDNA. ANSWER -33 ug/ml
optical density RNA. ANSWER -40 ug/ml
DNA concentration is calculated:. ANSWER -A260 x dilution factor x 50 ug/ml
RNA concentration =. ANSWER -A260 x dilution factor x 40 ug/ml
DNA B. ANSWER -helicase
DNA G. ANSWER -primase
Processivity. ANSWER -# of dNTPs that a polymerase can incorporate continuously
without dissociating from primer template
fidelity. ANSWER -error rate of polymerase
star activity. ANSWER -RE : additional cleavages at imperfect recognition sites
& ANSWERS WITH COMPLETE
SOLUTIONS GRADED A+
QUESTIONS AND ANSWERS
what does topoisomerase do?. ANSWER -breaks phosphate backbone to "un"
supercoil DNA
Another name for Topoisomerase II. ANSWER -Gyrase
Helicase. ANSWER -Un"zips" DNA at replication fork
What makes up the primosome?. ANSWER -1. primase
2. helicase
3. DNA C
NASBA stands for.... ANSWER -nucleic acid sequence based amplification
NASBA. ANSWER -RNA is target
RNA-->cDNA
Reverse transcriptase (RNA-->cDNA) followed by DNA dependent polymerase
(cDNA-->RNA)
RNAse H degrades original RNA template
,Evaluated gene EXPRESSION. ANSWER -RNA
ratio for organic extraction. ANSWER -Phenol (50): Chloroform (49): isoamyl alcohol
(1)
phenol. ANSWER -denatures proteins, dissolves lipids
chloroform. ANSWER -assists in dissolving remaining lipids, gives a clear interphase
between aqueous phase
Long term storage of RNA. ANSWER -70% EtOH at -70 C
ratio for 28S to 18S subunits. ANSWER -2:1 (can see this in a gel)
A260. ANSWER -nucleic acids
A280. ANSWER -protein
A230. ANSWER -organic compounds, phenol
A270. ANSWER -phenol
, optical density of ds DNA. ANSWER -50 ug/ml
optical density ssDNA. ANSWER -33 ug/ml
optical density RNA. ANSWER -40 ug/ml
DNA concentration is calculated:. ANSWER -A260 x dilution factor x 50 ug/ml
RNA concentration =. ANSWER -A260 x dilution factor x 40 ug/ml
DNA B. ANSWER -helicase
DNA G. ANSWER -primase
Processivity. ANSWER -# of dNTPs that a polymerase can incorporate continuously
without dissociating from primer template
fidelity. ANSWER -error rate of polymerase
star activity. ANSWER -RE : additional cleavages at imperfect recognition sites
