1 | Page
MLT (AMT) practice exam Questions
and Answers Latest 2026
Erythrocytes Ans: Cellular constituent of blood that
contains oxygen
ELISA Ans: Detects immune reponses
Westergren method Ans: Fresh anti-coagulated blood is
collected in Sodium citrate w/ratio of 4 vol. of blood to 1
vol. of sodium citrate
- blood should be at room temp and no longer than 2
hours old
-ESR
Megaloblastic anemia Ans: macrocytic anemia resulting
from B12 or folic acid deficiency or combination of both.
RNA Ans: - creates proteins via translation
-causes progressive decrease in both size and intensity of
the blue color in cell maturation in terms of cell size and
cytoplasm
New methylene blue Ans: helps precipitate residual
ribosomal RNA w/ reticulocytes
-binds, neutralizes and cross links RNA
© 2025 All rights reserved
, 2 | Page
Prussian Blue Ans: helps precipitate iron deposities in
liver tissue (biopsies) for distribution and iron amount
Myleoperoxidaes Stain Ans: shows cytoplasmic
immunoreactivity in neutrophilic + eosinophilic
granulocytes + their precursors
Specific esterase stain Ans: stains cells of granulocytic
origin
Chloroacetate esterase stain Ans: distinguish acute
leukemias that are granulocytic from leukemias that are
primarily of monocytic origin
Flow cytometry Ans: Detects and analyzed the chem and
physical characteristics of cell particles
-Suspends cells in fluid flow one at a time through a
focused beam of light
Impedance Ans: Automated blood cell counting principle
based on increased resistance that occurs when a blood
cell w/poor conductivity passes though an electrical field
Principal adult hemoglobins Ans: HbA - Hemoglobin A, 2
alpha + 2 beta chains
HbA2- Heoglobin A2, 2 alpha + 2 delta chains
-normally make up ~95% of hemoglobin in adults w/HgF
making up 5%or less
© 2025 All rights reserved
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Monocyte transition Ans: bone marrow -> pluripotent
stem cells -> monocyte->macrophage
Asynchronous maturation Ans: Megaloblastic
erythrocytes have an abnormal developmental sequence.
As they develop, cells of the megablastic sequence have a
more open or immature chromatin pattern in the
nucleus, referred to as
Troponin Ans: Gold standard of cardiac biomarkers for
determining myocardial infraction
Valinomycin Ans: Key membrane component for a
potassium selective electrode
Turbidimetry Ans: Measurement of decrease in intensity
of an incident light beam as it passes through a solution
of particles
Heterophil antibodies Ans: antibodies that are stimulated
by one antigen and react w/an entirely unrelated surface
antigen present on cells from different mammalian
species.
-titter of 1.56 or greater in clinically significant in
suspected cases
Neutrophil Maturation Ans: (bone marrow) Myloblast -
>promyelocyte (reddish purple granules)-> myelocyte -
>metamyelocyte -> Band -> segmented Neutrophil
Glomerulus Ans: Structure that delivers blood to the
nephron and is the site of urine formation
© 2025 All rights reserved
MLT (AMT) practice exam Questions
and Answers Latest 2026
Erythrocytes Ans: Cellular constituent of blood that
contains oxygen
ELISA Ans: Detects immune reponses
Westergren method Ans: Fresh anti-coagulated blood is
collected in Sodium citrate w/ratio of 4 vol. of blood to 1
vol. of sodium citrate
- blood should be at room temp and no longer than 2
hours old
-ESR
Megaloblastic anemia Ans: macrocytic anemia resulting
from B12 or folic acid deficiency or combination of both.
RNA Ans: - creates proteins via translation
-causes progressive decrease in both size and intensity of
the blue color in cell maturation in terms of cell size and
cytoplasm
New methylene blue Ans: helps precipitate residual
ribosomal RNA w/ reticulocytes
-binds, neutralizes and cross links RNA
© 2025 All rights reserved
, 2 | Page
Prussian Blue Ans: helps precipitate iron deposities in
liver tissue (biopsies) for distribution and iron amount
Myleoperoxidaes Stain Ans: shows cytoplasmic
immunoreactivity in neutrophilic + eosinophilic
granulocytes + their precursors
Specific esterase stain Ans: stains cells of granulocytic
origin
Chloroacetate esterase stain Ans: distinguish acute
leukemias that are granulocytic from leukemias that are
primarily of monocytic origin
Flow cytometry Ans: Detects and analyzed the chem and
physical characteristics of cell particles
-Suspends cells in fluid flow one at a time through a
focused beam of light
Impedance Ans: Automated blood cell counting principle
based on increased resistance that occurs when a blood
cell w/poor conductivity passes though an electrical field
Principal adult hemoglobins Ans: HbA - Hemoglobin A, 2
alpha + 2 beta chains
HbA2- Heoglobin A2, 2 alpha + 2 delta chains
-normally make up ~95% of hemoglobin in adults w/HgF
making up 5%or less
© 2025 All rights reserved
, 3 | Page
Monocyte transition Ans: bone marrow -> pluripotent
stem cells -> monocyte->macrophage
Asynchronous maturation Ans: Megaloblastic
erythrocytes have an abnormal developmental sequence.
As they develop, cells of the megablastic sequence have a
more open or immature chromatin pattern in the
nucleus, referred to as
Troponin Ans: Gold standard of cardiac biomarkers for
determining myocardial infraction
Valinomycin Ans: Key membrane component for a
potassium selective electrode
Turbidimetry Ans: Measurement of decrease in intensity
of an incident light beam as it passes through a solution
of particles
Heterophil antibodies Ans: antibodies that are stimulated
by one antigen and react w/an entirely unrelated surface
antigen present on cells from different mammalian
species.
-titter of 1.56 or greater in clinically significant in
suspected cases
Neutrophil Maturation Ans: (bone marrow) Myloblast -
>promyelocyte (reddish purple granules)-> myelocyte -
>metamyelocyte -> Band -> segmented Neutrophil
Glomerulus Ans: Structure that delivers blood to the
nephron and is the site of urine formation
© 2025 All rights reserved
