CMMB
CMMB
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Exam (elaborations)
CMMB 403 final exam | Q&A Latest 2024/2025 | 100% PASS
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--45January 20252024/2025A+Available in bundle
- CMMB 403 final exam | Q&A Latest 
2024/2025 | 100% PASS 
what does CRISPR use in order to generate changes in genome? is 
CRISPR forward or reverse genetics? - Answer -- nuclease 
- 20 nucleotide RNA sequence to target specific sites in the genome 
- reverse genetics 
what is the role of Cas9 in CRISPR - Answer -endonuclease that 
catalyzes double stranded breaks 
what are the two repair paths cells can use to repair double stranded 
breaks from CRISPR-Cas9 - Answer -1. non-homologous end joinin...
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AdelineJean
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Exam (elaborations)
CMMB 411 Final | Q&A Latest 2024/2025 | 100% PASS
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--56January 20252024/2025A+Available in bundle
- CMMB 411 Final | Q&A Latest 
2024/2025 | 100% PASS 
What is the Hi-C method? How do you do it? 
High-throughput chromosome conformation capture: Basically allows 
us to see what sequences are spatially close (not necessarily close in 
sequence). 
1. Cross-link spatially close DNA w formaldehyde 
2. digest using restriction fragments and fill in sticky ends with biotin 
labeled bases 
3. turn fragments into circular DNA (blunt end ligation) 
4. digest (shear) the DNA 
5. Used labeled beads to iso...
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AdelineJean
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Exam (elaborations)
CMMB 403 Final | Q&A Latest 2024/2025 | 100% PASS
-
--68January 20252024/2025A+Available in bundle
- CMMB 403 Final | Q&A Latest 
2024/2025 | 100% PASS 
What is the developmental gene hierarchy in drosophila? What do each 
of them do? Give all examples for each 
1. Maternal effect genes: set up early polarity (bicoid, nanos, caudal, 
hunchback) 
2. Gap genes: Create 1-2 large general regions (Kruppel, knirps, giant, 
hunchback) 
3. Pair-rule: Create 7 segments (even-skipped, Paired, Fushi tarazu) 
4. Segment polarity: Give all 14 segments polarity (Engrailed, SHH, 
wingless) 
5. Hox genes: Give...
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AdelineJean
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Exam (elaborations)
CMMB 343 lab final | Q&A Latest 2024/2025 | 100% PASS
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--26January 20252024/2025A+Available in bundle
- CMMB 343 lab final | Q&A Latest 
2024/2025 | 100% PASS 
How to do ..... 
BHI stant 
How to interpret results? - Answer -Slanted BHI agar in a test tube 
Inoculate with the sample 
Will see growth on surface of agar if aerobic microbe. 
Fissures and cracks below surface if able to ferment. 
Deep shake culture - Answer -Innoculate a loop of sample in molten 
agar and rotate in hands to mix. 
If fissures below the surface, can ferment. 
If no fissures, cannot ferment.Thioglycollate Medium - Answer ...
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AdelineJean
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Exam (elaborations)
CMMB 403 - Final Exam Review | Q&A Latest 2024/2025 | 100% PASS
-
--73January 20252024/2025A+Available in bundle
- CMMB 403 - Final Exam Review | 
Q&A Latest 2024/2025 | 100% 
PASS 
Which animal's development is chick development most similar to 
compared to other species? - Answer -Most similar to humans than 
other species talked about in lecture (like amphibians and flies) 
What is the Nieuwkoop Centre (NC)? When does it emerge during 
embryo development? - Answer -Centre that induces the formation of 
the organizer and later the dorsal mesoderm; emerges during the late 
blastula stage 
How does the fi...
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AdelineJean