bios390

BIOS390 Molecular Biology Week 7 Study Guide

BIOS 390 Week 5 LAB: Transcription in Prokaryotes-Verified/BIOS 390 Week 5 LAB/Transcription is the first step in gene expression. It involves copying a gene’s DNA sequence to make an RNA molecule. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble. Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA produ...

BIOS 390 Week 3 LAB: DNA Cloning-100% Verified-DNA cloning is the process of making multiple, identical copies of a certain piece of DNA. A gene or other DNA fragment of interest is first inserted into a circular piece of DNA called a plasmid. The insertion is done using enzymes that are able to “cut and paste” DNA, and then a molecule of recombinant DNA is produced. Next, the recombinant plasmid is introduced into bacteria. Bacteria carrying the plasmid are selected and grown up. As they re...

BIOS 390 Week 2 LAB 2: Gel Electrophoresis-Latest Update/Gel electrophoresis is a laboratory technique used to separate DNA fragments and other macromolecules by size and charge. Gel electrophoresis is a technique in which fragments of DNA are … through a gel matrix by an electric current, and it separates DNA fragments according to size. A standard, or DNA ladder, is typically … so that the size of the fragments in the PCR sample can … determined. The results of a PCR reaction are usually...