Organic Chemistry

Antioxidants scavenge free radicals. Though free radicals are freely availble in human body , excess amounts of free radicals cause oxidative stress. Phenols and flavonoids are natural antioxidants. Vitamin C, vitamin E, carotenoids, phenolic componds are some examples for natural antioxidants. Hydroxyl groups of phenols confer scavenging activity. Total phenolic content is determined using F-C method. The sample changes its color from yellow to green/ intense blue after addition of F-C reagent....

Free radicals (ROS, RNS) are freely available in human body. But excess amounts of free radicals cause damages to the human body such as diabetes mellitus, stroke and Alzheimer's disease. Antioxidants effectively scavenge free radicals. Phenolic compounds and flavonoids are natural antioxidants. Availability of phenolic compounds and flavonoids can be confirmed by folin ciocateu method and aluminium colorimetric method respectively. Folin -ciocateu method forms a blue chromophore is Phosphotung...

Oxidative metabolism is essential for the survival of cells. However, it results in free radicals and reactive oxygen species (ROS) that causes oxidative damage if large amounts exist in the body. Imbalance between ROS and cellular antioxidant capacity leads to oxidative stress. If this condition is not properly regulated by the body it will results many diseases such as cancer, stroke and heart disease.Antioxidants scavenge free radicals. Phenols and flavonoids are natural antioxidants. Availab...

Free radicals are freely available in human body (ROS, RON). But excess amount of free radicals cause damage to human body resulting many diseases such as diabetes mellitus, stroke and Alzheimer's disease. Antioxidants scavenge free radicals. Phenolic compounds and flavonoids are effective natural free radical scavengers. TPC assay and TFC assay confirm the presence of phenolic derivatives and flavonoids in a sample. In TPC assay, results are expressed as milligrams of pyrogallol equivalent (P...