|QUESTIONS AND 100% CORRECT WELL DETAILED
Thioglycollate broth is stored at room temperature and in the dark so that? -
ANSWER There is a decreased absorption of oxygen by the medium
A liquid fecal specimen from a 3-month-old infant is submitted for a microbiologic
examination. In addition to a culture on routine media for Salmonella and Shigella, this
specimen routinely should be? - ANSWER examined for the presence of
campylobacter species
An organism was inoculated in a TSI tube and gave the following reactions:
alkaline slant/acid butt
H2s Gas produced
The organism is most likely? - ANSWER Salmonella typhimurium
Failure to obtain anaerobiosis in anaerobe jars is most often due to the? -
ANSWER inactivation of the palladium-coated illumine catalysts pellets
Which specimen request is acceptable? - ANSWER urine culture for acid-fast bacilli
Gram-Positive rod was isolated from a wound infection caused by a bite from a pet cat. The
following characteristics were seen:
Oxidase positive
Glucose OF fermentative
catalase positive
1
, motility negative
MacConkey agar no growth
This organism is most likely? - ANSWER Pasteurella multocida
A 10-year-old boy was admitted to the emergency room with appendicitis. The following
laboratory results were obtained:
% of segmented neutrophils Patient: 75 range: 16-60
WBC count Pt: 200-103/uL R: 5.0-10.0 uL x 103/uL
During surgery the appendix appeared normal; an enlarged node was removed and cultured.
Small GNR were isolated from BAP & MAC. The organisms grew better at room temperature.
The organism is most likely? - ANSWER Yersinia enterocolitica
Which of the following is most often used to prepare a slide from a plate culture of
dermatophyte for microscopic examination? - ANSWER lactophenol cotton blue
What is the most useful morphologic feature in identifying the mycelial phase Histoplasma
capsulatum? - ANSWER tuberculate microconidia 8-14 microns
The function of N-acetyl-L-cysteine required in the NALC-NaOH reagent for acid-fast
digestion-decontamination procedure is to? - ANSWER liquefy the mucous
Middlebrook 7H10 and 7H11 media must be refrigerated in the dark and incubated in the
dark as well. If these conditions are not met, the media may prove toxic for mycobacteria
because? - ANSWER formaldehyde may be produced
Ureaplasma urealyticum are difficult to grow in the laboratory on routine media because of
their requirement for? - ANSWER sterols
The cyst state may be recovered from fecal specimens submitted for parasitic examinations if
the specimen? - ANSWER is stored at refrigerated temperature
2
Thioglycollate broth is stored at room temperature and in the dark so that? -
ANSWER There is a decreased absorption of oxygen by the medium
A liquid fecal specimen from a 3-month-old infant is submitted for a microbiologic
examination. In addition to a culture on routine media for Salmonella and Shigella, this
specimen routinely should be? - ANSWER examined for the presence of
campylobacter species
An organism was inoculated in a TSI tube and gave the following reactions:
alkaline slant/acid butt
H2s Gas produced
The organism is most likely? - ANSWER Salmonella typhimurium
Failure to obtain anaerobiosis in anaerobe jars is most often due to the? -
ANSWER inactivation of the palladium-coated illumine catalysts pellets
Which specimen request is acceptable? - ANSWER urine culture for acid-fast bacilli
Gram-Positive rod was isolated from a wound infection caused by a bite from a pet cat. The
following characteristics were seen:
Oxidase positive
Glucose OF fermentative
catalase positive
1
, motility negative
MacConkey agar no growth
This organism is most likely? - ANSWER Pasteurella multocida
A 10-year-old boy was admitted to the emergency room with appendicitis. The following
laboratory results were obtained:
% of segmented neutrophils Patient: 75 range: 16-60
WBC count Pt: 200-103/uL R: 5.0-10.0 uL x 103/uL
During surgery the appendix appeared normal; an enlarged node was removed and cultured.
Small GNR were isolated from BAP & MAC. The organisms grew better at room temperature.
The organism is most likely? - ANSWER Yersinia enterocolitica
Which of the following is most often used to prepare a slide from a plate culture of
dermatophyte for microscopic examination? - ANSWER lactophenol cotton blue
What is the most useful morphologic feature in identifying the mycelial phase Histoplasma
capsulatum? - ANSWER tuberculate microconidia 8-14 microns
The function of N-acetyl-L-cysteine required in the NALC-NaOH reagent for acid-fast
digestion-decontamination procedure is to? - ANSWER liquefy the mucous
Middlebrook 7H10 and 7H11 media must be refrigerated in the dark and incubated in the
dark as well. If these conditions are not met, the media may prove toxic for mycobacteria
because? - ANSWER formaldehyde may be produced
Ureaplasma urealyticum are difficult to grow in the laboratory on routine media because of
their requirement for? - ANSWER sterols
The cyst state may be recovered from fecal specimens submitted for parasitic examinations if
the specimen? - ANSWER is stored at refrigerated temperature
2
