Biology 113 Exam Questions and answers with complete solutions Latest Updated 2025 |
Verified
What should you do if the fire alarm rings? - 1. stop experiment
2. if applicable, close windows
3. line up in orderly fashion
4. proceed down steps with last person closing the door
T of F: use a kimwipe to clean the microscope lens - False, use gauze
What happens if an animal cell is placed in a hypertonic solution? - the cell will shrivel
What happens if an animal cell is placed in a hypotonic solution? - the cell will burst
According to the "Selling soap" article when did doctors finally begin to comply washing their hands? -
when they were shown a picture of the bacteria culture from their hands
Peroxidase from turnips is a ? - enzyme
Hydrogen peroxide is a ? - substrate
What color is guaiacol in its oxidized state? - yellow
What color is DCPIP in its oxidized state? - purple
Compare the leaves when grown in the dark and in the light when their pigment was removed. - Dark:
the leaf is brown and dark; no starch present
light: the leaf is yellow; starch present
Compare the tobacco seeds when grown in the light and dark. - Light: seeds were green
, Dark: seeds were albino
Height and weight/shape in dogs is variable within breeds. This suggests that traits in dogs are -
polymorphic
Three stages of PCR - denaturation (94˚C), annealing (60˚C), extension (72˚C)
iris diaphragm - controls the amount of light passing through the specimen
Parfocality - remains close in focus as it switches from objective to objective, minor adjustments with
the fine focus knob may be necessary
when do you use immersion oil? - 100x lens
binocular dissecting light microscope - also known as dissecting microscope: often used for low
magnification of plate cultures, or for structures that are viewed as live specimens and/or too large to
view under a standard compound microscope; magnifications range from 10x to 25x or 7x to 30x
when defining a solution as iso, hypo, and hypertonic, what process does this relate to? - Osmosis: the
diffusion of water through a semi permeable membrane to an area of higher concentration
Diffusion - Movement of molecules from an area of higher concentration to an area of lower
concentration.
Can osmosis and diffusion occur at the same time? - Yes. Ex: cell solution contained NaCl and a beaker
contained water; NaCl moved into the beaker (diffusion) and water moved into the bag (osmosis)
T or F: 150 mM NaCl and 300mM glucose are osmotic equivalents - True
In the enzyme activity, what is the last solution added to the test tube? Why? - two dropped of
substrate (hydrogen peroxide); this must be added last because the solution starts reacting and must be
covered by Parafilm and placed into the spec for an absorbance reading
Verified
What should you do if the fire alarm rings? - 1. stop experiment
2. if applicable, close windows
3. line up in orderly fashion
4. proceed down steps with last person closing the door
T of F: use a kimwipe to clean the microscope lens - False, use gauze
What happens if an animal cell is placed in a hypertonic solution? - the cell will shrivel
What happens if an animal cell is placed in a hypotonic solution? - the cell will burst
According to the "Selling soap" article when did doctors finally begin to comply washing their hands? -
when they were shown a picture of the bacteria culture from their hands
Peroxidase from turnips is a ? - enzyme
Hydrogen peroxide is a ? - substrate
What color is guaiacol in its oxidized state? - yellow
What color is DCPIP in its oxidized state? - purple
Compare the leaves when grown in the dark and in the light when their pigment was removed. - Dark:
the leaf is brown and dark; no starch present
light: the leaf is yellow; starch present
Compare the tobacco seeds when grown in the light and dark. - Light: seeds were green
, Dark: seeds were albino
Height and weight/shape in dogs is variable within breeds. This suggests that traits in dogs are -
polymorphic
Three stages of PCR - denaturation (94˚C), annealing (60˚C), extension (72˚C)
iris diaphragm - controls the amount of light passing through the specimen
Parfocality - remains close in focus as it switches from objective to objective, minor adjustments with
the fine focus knob may be necessary
when do you use immersion oil? - 100x lens
binocular dissecting light microscope - also known as dissecting microscope: often used for low
magnification of plate cultures, or for structures that are viewed as live specimens and/or too large to
view under a standard compound microscope; magnifications range from 10x to 25x or 7x to 30x
when defining a solution as iso, hypo, and hypertonic, what process does this relate to? - Osmosis: the
diffusion of water through a semi permeable membrane to an area of higher concentration
Diffusion - Movement of molecules from an area of higher concentration to an area of lower
concentration.
Can osmosis and diffusion occur at the same time? - Yes. Ex: cell solution contained NaCl and a beaker
contained water; NaCl moved into the beaker (diffusion) and water moved into the bag (osmosis)
T or F: 150 mM NaCl and 300mM glucose are osmotic equivalents - True
In the enzyme activity, what is the last solution added to the test tube? Why? - two dropped of
substrate (hydrogen peroxide); this must be added last because the solution starts reacting and must be
covered by Parafilm and placed into the spec for an absorbance reading
