(MB) ASCP
PRACTICE EXAM
QUESTIONS WITH
100% CORRECT
ANSwers
1|Page
, (MB) ASCP Practice Exam Questions With 100% Correct
Answers Verified (2023-2024) (155 Questions)
1.Which of the following is not a component of a
nucleotide? Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answers ✅Anti-codon
2.According to Chargaff's rule of base pairing, adenine pairs with: - correct
answers ✅Thymine
3.What genes would be screened in a breast cancer panel? - correct answers
✅HER2, ERBB2, BRCA1
4.Next Generation Sequencing uses: - correct answers ✅Short sequence
reads
5.Purines and pyrimidines differ from each other in that: - correct answers
✅Purines have two rings; pyrimidines have one ring
6.The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers ✅Adenine and guanine
2|Page
, (MB) ASCP Practice Exam Questions With 100% Correct
Answers Verified (2023-2024) (155 Questions)
1.What is the rate of mutation per round of DNA
replication? 1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers ✅1 in 1,000,000,000 base
pairs
2.The rate of DNA migration through an agarose gel during
electrophoresis does not depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers ✅Nucleotide
sequence of the molecule
3.What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
3|Page
, (MB) ASCP Practice Exam Questions With 100% Correct
Answers Verified (2023-2024) (155 Questions)
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers
✅Initiation, exponential, plateau
4.Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers ✅3'-GAC
5.A patient with impaired judgment, personality changes, signs of
abnormal body movements and depression comes to the physician's
office for a follow-up visit. The physician suspects a single-gene disorder
may be the cause of those clinical manifestations. A blood specimen was
then sent to your clinical laboratory for mutation screening in the
Huntington gene.
Testing with standard PCR indicates that patient has Huntington Disease, HD.
Which of the following would be consistent with this diagnosis?
4|Page
PRACTICE EXAM
QUESTIONS WITH
100% CORRECT
ANSwers
1|Page
, (MB) ASCP Practice Exam Questions With 100% Correct
Answers Verified (2023-2024) (155 Questions)
1.Which of the following is not a component of a
nucleotide? Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answers ✅Anti-codon
2.According to Chargaff's rule of base pairing, adenine pairs with: - correct
answers ✅Thymine
3.What genes would be screened in a breast cancer panel? - correct answers
✅HER2, ERBB2, BRCA1
4.Next Generation Sequencing uses: - correct answers ✅Short sequence
reads
5.Purines and pyrimidines differ from each other in that: - correct answers
✅Purines have two rings; pyrimidines have one ring
6.The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers ✅Adenine and guanine
2|Page
, (MB) ASCP Practice Exam Questions With 100% Correct
Answers Verified (2023-2024) (155 Questions)
1.What is the rate of mutation per round of DNA
replication? 1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers ✅1 in 1,000,000,000 base
pairs
2.The rate of DNA migration through an agarose gel during
electrophoresis does not depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers ✅Nucleotide
sequence of the molecule
3.What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
3|Page
, (MB) ASCP Practice Exam Questions With 100% Correct
Answers Verified (2023-2024) (155 Questions)
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers
✅Initiation, exponential, plateau
4.Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers ✅3'-GAC
5.A patient with impaired judgment, personality changes, signs of
abnormal body movements and depression comes to the physician's
office for a follow-up visit. The physician suspects a single-gene disorder
may be the cause of those clinical manifestations. A blood specimen was
then sent to your clinical laboratory for mutation screening in the
Huntington gene.
Testing with standard PCR indicates that patient has Huntington Disease, HD.
Which of the following would be consistent with this diagnosis?
4|Page
