Solutions Manual For Quantitative Analysis
For Management14th Edition 2024/2025
updated on Jul 11 2024(chapter 1) with
Correct Questions And Answers (ALASKA
PACIFIC UNIVERSITY)
, 2. What feature is commonly seen in the sequences recognized by type II restriction
enzymes? - ANS✔✔--Solution:
The recognition sequences are palindromic, and 4−8 base pairs long.
3. What normal role do restriction enzymes play in bacteria? How do bacteria protect
their own DNA from the action of restriction enzymes? - ANS✔✔--Solution:
Restriction enzymes cut foreign DNA, such as viral DNA, into fragments. Bacteria
protect their own DNA by modifying bases, usually by methylation, at the recognition
sites.
4. Explain how gel electrophoresis is used to separate DNA fragments of different
lengths. - ANS✔✔--Solution:
Gel electrophoresis uses an electric field to drive DNA molecules through a gel that acts
as a molecular sieve. The gel is an aqueous matrix of agarose or polyacrylamide. DNA
molecules are loaded into a slot or well at one end of the gel. When an electric field is
applied, the negatively charged DNA molecules migrate toward the positive electrode.
Shorter DNA molecules are less hindered by the agarose or polyacrylamide matrix and
migrate faster than longer DNA molecules, which must wind their way around obstacles
and through the pores in the gel matrix.
5. After DNA fragments have been separated by gel electrophoresis, how can they be
visualized? - ANS✔✔--Solution:
DNA molecules can be visualized by staining with a fluorescent dye, such as ethidium
bromide, that intercalates between the stacked bases of the DNA double helix, and the
dye-DNA complex fluoresces when irradiated with an ultraviolet light source.
Alternatively, they can be visualized by attaching radioactive or chemical labels to the
DNA before it is placed in the gel.
6. What is the purpose of Southern blotting? How is it carried out? - ANS✔✔--
Solution:
Southern blotting is used to detect and visualize specific DNA fragments that have a
sequence complementary to a labeled DNA probe. DNA is first cleaved into fragments
with restriction endonucleases. The fragments are separated by size via gel
electrophoresis. These fragments are then denatured and transferred by blotting onto
For Management14th Edition 2024/2025
updated on Jul 11 2024(chapter 1) with
Correct Questions And Answers (ALASKA
PACIFIC UNIVERSITY)
, 2. What feature is commonly seen in the sequences recognized by type II restriction
enzymes? - ANS✔✔--Solution:
The recognition sequences are palindromic, and 4−8 base pairs long.
3. What normal role do restriction enzymes play in bacteria? How do bacteria protect
their own DNA from the action of restriction enzymes? - ANS✔✔--Solution:
Restriction enzymes cut foreign DNA, such as viral DNA, into fragments. Bacteria
protect their own DNA by modifying bases, usually by methylation, at the recognition
sites.
4. Explain how gel electrophoresis is used to separate DNA fragments of different
lengths. - ANS✔✔--Solution:
Gel electrophoresis uses an electric field to drive DNA molecules through a gel that acts
as a molecular sieve. The gel is an aqueous matrix of agarose or polyacrylamide. DNA
molecules are loaded into a slot or well at one end of the gel. When an electric field is
applied, the negatively charged DNA molecules migrate toward the positive electrode.
Shorter DNA molecules are less hindered by the agarose or polyacrylamide matrix and
migrate faster than longer DNA molecules, which must wind their way around obstacles
and through the pores in the gel matrix.
5. After DNA fragments have been separated by gel electrophoresis, how can they be
visualized? - ANS✔✔--Solution:
DNA molecules can be visualized by staining with a fluorescent dye, such as ethidium
bromide, that intercalates between the stacked bases of the DNA double helix, and the
dye-DNA complex fluoresces when irradiated with an ultraviolet light source.
Alternatively, they can be visualized by attaching radioactive or chemical labels to the
DNA before it is placed in the gel.
6. What is the purpose of Southern blotting? How is it carried out? - ANS✔✔--
Solution:
Southern blotting is used to detect and visualize specific DNA fragments that have a
sequence complementary to a labeled DNA probe. DNA is first cleaved into fragments
with restriction endonucleases. The fragments are separated by size via gel
electrophoresis. These fragments are then denatured and transferred by blotting onto
