2025 Pearson Edexcel Level 3 GCE Biology B Advanced PAPER 2: Advanced Physiology, Evolution and Ecology Combined
Question Paper and Marking Scheme
Please check the examination details below before entering your candidate information
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Friday 13 June 2025
Morning (Time: 1 hour 45 minutes) Paper
reference 9BI0/02
Biology B
Advanced
PAPER 2: Advanced Physiology, Evolution and Ecology
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
• Use black ink or ball-point pen.
• Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer
Answer all questions.
the questions in the spaces provided
– there may be more space than you need.
Information
• The total mark for this paper is 90.
• The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• Instructure
question(s) marked with an asterisk (*), marks will be awarded for your ability to
your answer logically showing how the points that you make are related or
follow on from each other where appropriate.
Advice
• Read each question carefully before you start to answer it.
•• Check
Try to answer every question.
your answers if you have time at the end.
Turn over
P74460A
©2025 Pearson Education Ltd.
Y:1/1/1/1/1/1/1/1/
, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 The photograph shows part of a liver cell, as seen using an electron microscope.
mitochondrion
rough
endoplasmic
reticulum Golgi apparatus
DO NOT WRITE IN THIS AREA
(Source: © Keith R. Porter/SCIENCE PHOTO LIBRARY)
(a) Describe the roles of the labelled organelles in a liver cell.
(3)
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2
, (b) Hepatitis B is a DNA virus that infects liver cells. The DNA of the virus replicates
inside liver cells.
(i) Describe the process of DNA replication.
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(3)
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3
Turn over
, (ii) The drug lamivudine is used to treat infections by the hepatitis B virus.
It causes the production of altered nucleotides containing cytosine.
DO NOT WRITE IN THIS AREA
The diagram shows a normal nucleotide and an altered nucleotide.
NH2
NH2
N
O O N
HO P O N O
O HO P O N O
OH O
OH
OH
S
normal nucleotide altered nucleotide
DO NOT WRITE IN THIS AREA
Explain how lamivudine reduces replication of the hepatitis B virus in
liver cells.
(2)
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(Total for Question 1 = 8 marks) DO NOT WRITE IN THIS AREA
4
Question Paper and Marking Scheme
Please check the examination details below before entering your candidate information
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Friday 13 June 2025
Morning (Time: 1 hour 45 minutes) Paper
reference 9BI0/02
Biology B
Advanced
PAPER 2: Advanced Physiology, Evolution and Ecology
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
• Use black ink or ball-point pen.
• Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer
Answer all questions.
the questions in the spaces provided
– there may be more space than you need.
Information
• The total mark for this paper is 90.
• The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• Instructure
question(s) marked with an asterisk (*), marks will be awarded for your ability to
your answer logically showing how the points that you make are related or
follow on from each other where appropriate.
Advice
• Read each question carefully before you start to answer it.
•• Check
Try to answer every question.
your answers if you have time at the end.
Turn over
P74460A
©2025 Pearson Education Ltd.
Y:1/1/1/1/1/1/1/1/
, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 The photograph shows part of a liver cell, as seen using an electron microscope.
mitochondrion
rough
endoplasmic
reticulum Golgi apparatus
DO NOT WRITE IN THIS AREA
(Source: © Keith R. Porter/SCIENCE PHOTO LIBRARY)
(a) Describe the roles of the labelled organelles in a liver cell.
(3)
............................................................................................................................... ............................................................................................................................... ......................
............................................................................................................................... ............................................................................................................................... ......................
DO NOT WRITE IN THIS AREA
............................................................................................................................... ............................................................................................................................... ......................
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............................................................................................................................... ............................................................................................................................... ......................
2
, (b) Hepatitis B is a DNA virus that infects liver cells. The DNA of the virus replicates
inside liver cells.
(i) Describe the process of DNA replication.
DO NOT WRITE IN THIS AREA
(3)
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............................................................................................................................... ............................................................................................................................... ......................
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DO NOT WRITE IN THIS AREA
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DO NOT WRITE IN THIS AREA
3
Turn over
, (ii) The drug lamivudine is used to treat infections by the hepatitis B virus.
It causes the production of altered nucleotides containing cytosine.
DO NOT WRITE IN THIS AREA
The diagram shows a normal nucleotide and an altered nucleotide.
NH2
NH2
N
O O N
HO P O N O
O HO P O N O
OH O
OH
OH
S
normal nucleotide altered nucleotide
DO NOT WRITE IN THIS AREA
Explain how lamivudine reduces replication of the hepatitis B virus in
liver cells.
(2)
............................................................................................................................... ............................................................................................................................... ......................
............................................................................................................................... ............................................................................................................................... ......................
............................................................................................................................... ............................................................................................................................... ......................
............................................................................................................................... ............................................................................................................................... ......................
............................................................................................................................... ............................................................................................................................... ......................
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(Total for Question 1 = 8 marks) DO NOT WRITE IN THIS AREA
4
