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HTL (Histotechnologist) Bundle Set
HTL (Histotechnologist) Bundle Set
[Montrer plus]HTL (Histotechnologist) Bundle Set
[Montrer plus]tissue is fix in order to - Answer stop enzymatic action 
 
which fixatives contains picric acid, formalin, and acetic acid - Answer bouin 
 
the volume of fixative should exceed the volume of the tissue by - Answer to to 20 times 
 
tissue must be washed in running water after fixation in - A...
Aperçu 1 sur 4 pages
Ajouter au paniertissue is fix in order to - Answer stop enzymatic action 
 
which fixatives contains picric acid, formalin, and acetic acid - Answer bouin 
 
the volume of fixative should exceed the volume of the tissue by - Answer to to 20 times 
 
tissue must be washed in running water after fixation in - A...
Alcian Blue demonstrates - Answer Acid mucins 
 
ATPase Enyme Histochemistry demonstrates - Answer ATPase in muscles 
 
Bielschowsky's Silver Technique demonatrates - Answer Dendrites, axons, neurofibrils 
 
Brown & Brenn gram stain demonstrates - Answer bacteria 
 
Congo red demonstrat...
Aperçu 4 sur 178 pages
Ajouter au panierAlcian Blue demonstrates - Answer Acid mucins 
 
ATPase Enyme Histochemistry demonstrates - Answer ATPase in muscles 
 
Bielschowsky's Silver Technique demonatrates - Answer Dendrites, axons, neurofibrils 
 
Brown & Brenn gram stain demonstrates - Answer bacteria 
 
Congo red demonstrat...
The primary purpose of fixation is - Answer To stop enzymatic action 
 
Which fixative contains picric acid, formalin, and acetic acid? - Answer Bouin 
 
The volume of fixative should exceed the volume of the tissue by - Answer 15-20 times 
 
Tissue must be washed in water after fixation in - ...
Aperçu 4 sur 98 pages
Ajouter au panierThe primary purpose of fixation is - Answer To stop enzymatic action 
 
Which fixative contains picric acid, formalin, and acetic acid? - Answer Bouin 
 
The volume of fixative should exceed the volume of the tissue by - Answer 15-20 times 
 
Tissue must be washed in water after fixation in - ...
Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. 
 
Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Use...
Aperçu 2 sur 6 pages
Ajouter au panierAcetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. 
 
Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Use...
What is the most important factor that would contribute to tissue firmness when cutting on the cryostat? - Answer Lipid and water content of tissue 
 
If you are making 1000 ml of a 0.25% acetic acid solution. Which instrument would you use to transfer the acetic acid? - Answer 5ml pipette 
 
If...
Aperçu 2 sur 6 pages
Ajouter au panierWhat is the most important factor that would contribute to tissue firmness when cutting on the cryostat? - Answer Lipid and water content of tissue 
 
If you are making 1000 ml of a 0.25% acetic acid solution. Which instrument would you use to transfer the acetic acid? - Answer 5ml pipette 
 
If...
used to determine the origin of a tumor, prognosis and treatment. - Answer Immunohistochemistry 
 
is a host protein (immunoglobulin) produced in response to the presence of foreign molecules, organisms, or other agent in the body. - Answer antibody 
 
Immunoglobulins are proteins produced by ...
Aperçu 2 sur 8 pages
Ajouter au panierused to determine the origin of a tumor, prognosis and treatment. - Answer Immunohistochemistry 
 
is a host protein (immunoglobulin) produced in response to the presence of foreign molecules, organisms, or other agent in the body. - Answer antibody 
 
Immunoglobulins are proteins produced by ...
Endoplasmic Reticulum - Answer H&E basophilic staining within cytoplasm is due to what cellular structure? 
 
Pink - Answer Cytoplasm will show up what color on an H&E? 
 
20 - Answer Proteins are made up of combinations of how many amino acids? 
 
Carboxyl group, side group - Answer All...
Aperçu 2 sur 10 pages
Ajouter au panierEndoplasmic Reticulum - Answer H&E basophilic staining within cytoplasm is due to what cellular structure? 
 
Pink - Answer Cytoplasm will show up what color on an H&E? 
 
20 - Answer Proteins are made up of combinations of how many amino acids? 
 
Carboxyl group, side group - Answer All...
Dehydrating Agents - Answer Alcohol, dioxane, acetone, tetrahydrofuran. 
 
Clearing Agents - Answer Xylene, toluene, benzene, acetone, chloroform, cedarwood oil, tetrahydrofuran, dioxane, limonene, aliphatic hydrocarbons. 
 
Universal Solvent - Answer Solvents that perform both the dehydration...
Aperçu 1 sur 2 pages
Ajouter au panierDehydrating Agents - Answer Alcohol, dioxane, acetone, tetrahydrofuran. 
 
Clearing Agents - Answer Xylene, toluene, benzene, acetone, chloroform, cedarwood oil, tetrahydrofuran, dioxane, limonene, aliphatic hydrocarbons. 
 
Universal Solvent - Answer Solvents that perform both the dehydration...
Polarizing Microscope - Answer Microscope used to identify crystals, talc, silica, or urates. 
 
Birefringence - Answer Transmitting light unequally in different directions- 
 
Phase-Contrast Microscope - Answer Microscope used to examine unstained objects. Not routinely used in histopath labs...
Aperçu 1 sur 2 pages
Ajouter au panierPolarizing Microscope - Answer Microscope used to identify crystals, talc, silica, or urates. 
 
Birefringence - Answer Transmitting light unequally in different directions- 
 
Phase-Contrast Microscope - Answer Microscope used to examine unstained objects. Not routinely used in histopath labs...
Cresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate. 
 
Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate. 
 
Bodain Method for Nerve Fibers - Answer Protargol (silver solutio...
Aperçu 1 sur 2 pages
Ajouter au panierCresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate. 
 
Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate. 
 
Bodain Method for Nerve Fibers - Answer Protargol (silver solutio...
Too much blade tilt causes this:? - Answer Chatter 
 
Chatter may be caused by? - Answer Cutting too rapidly 
 
Naphthol AS-D chloroacetate esterase stains are rare among enzyme techniques since it may be used on these type of sections? - Answer Formalin fixed paraffin
Aperçu 1 sur 2 pages
Ajouter au panierToo much blade tilt causes this:? - Answer Chatter 
 
Chatter may be caused by? - Answer Cutting too rapidly 
 
Naphthol AS-D chloroacetate esterase stains are rare among enzyme techniques since it may be used on these type of sections? - Answer Formalin fixed paraffin
Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. 
 
Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. U...
Aperçu 3 sur 17 pages
Ajouter au panierAcetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. 
 
Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. U...
Cryptococcus neoformans - Answer a fungus that can be demonstrated with various stains for acidic mucins is 
 
the P.H. should be modified on the 4.6 stain - Answer ATPase stains done on a muscle biopsy at P.H.9.4,4.6 & 4.3.The slides for P.H.9.4 & 4.3 show good differentiation of the type 1 &...
Aperçu 2 sur 9 pages
Ajouter au panierCryptococcus neoformans - Answer a fungus that can be demonstrated with various stains for acidic mucins is 
 
the P.H. should be modified on the 4.6 stain - Answer ATPase stains done on a muscle biopsy at P.H.9.4,4.6 & 4.3.The slides for P.H.9.4 & 4.3 show good differentiation of the type 1 &...
This is a stain for acid mucopolysaccharides and sialomucins. - Answer Alcian blue 
 
This stains reacts with cations and is used to find calcium in tissue. - Answer Alizaran Red S 
 
This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - A...
Aperçu 2 sur 8 pages
Ajouter au panierThis is a stain for acid mucopolysaccharides and sialomucins. - Answer Alcian blue 
 
This stains reacts with cations and is used to find calcium in tissue. - Answer Alizaran Red S 
 
This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - A...
Glycogen is most abundant in - Answer Liver, heart (cardiac muscle cells), womb 
 
Staining reactions for glycogen: - Answer PAS (+), diastase (sensitive), Bauer-feulgen (+), Best carmine (+) 
 
Which two glycogen fixatives preserves the most glycogen? - Answer Bouins & Rossman's 
 
Other gly...
Aperçu 2 sur 15 pages
Ajouter au panierGlycogen is most abundant in - Answer Liver, heart (cardiac muscle cells), womb 
 
Staining reactions for glycogen: - Answer PAS (+), diastase (sensitive), Bauer-feulgen (+), Best carmine (+) 
 
Which two glycogen fixatives preserves the most glycogen? - Answer Bouins & Rossman's 
 
Other gly...
Most coagulant fixatives achieve their full effect on tissue at any particular depth as soon as... - Answer they have penetrated to that depth at a concentration sufficient to cause coagulation. 
 
Fixative - Answer alters tissue by stabilizing the protein so that it is resistant to further chan...
Aperçu 4 sur 41 pages
Ajouter au panierMost coagulant fixatives achieve their full effect on tissue at any particular depth as soon as... - Answer they have penetrated to that depth at a concentration sufficient to cause coagulation. 
 
Fixative - Answer alters tissue by stabilizing the protein so that it is resistant to further chan...
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