Study Guide for Exam 2 |
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, chapter 6: micro, MICRO Exam 2, Ch.
13 Metabolism Textbook Q's, Micro
Biology chapter 13: Metabolism,
Biology 2EE3 - Chapter 13, Bio 1 Ch7
Questions, chapter 7, BIOl 1100 ch. 8
test review, BIOL 2051 - Ch. 4 Testbank
(EXAM 1), chapter 4
Question 1
Which one of the following elements is considered a microelement, usually required
in minute amounts in microbiological media? Correct Answer
manganese
Question 2
What is the function of siderophores?
Correct Answer
Binding iron for transport into the cell.
Question 3
An autotroph is defined as an organism that uses _____ as a source of _____.
Correct Answer carbon dioxide; carbon
Question 4
A heterotroph is defined as an organism that uses _____ as a source of
_____. Correct Answer organic compounds; carbon
Question 5
What is the most readily useable form of nitrogen by
microorganisms? Correct Answer ammonium ion
Question 6
What is nitrogen fixation?
Correct Answer
The reduction of dinitrogen to ammonia for assimilation.
,Question 7
What is the most abundant form of nitrogen in the
biosphere? Correct Answer dinitrogen
Question 8
A phototroph is defined as an organism that uses _____ as a source of _____.
Correct Answer sunlight; energy
Question 9
9) A lithotroph is defined as an organism that uses _____ as a source of _____.
Correct Answer inorganic compounds; energy and electrons
Question 10
10) What is the most common solidifying agent used in microbiological
media? Correct Answer agar
Question 11
11) Which one of the following is NOT a physical factor that affects the growth
rate of cells? Correct Answer nutrient concentration
Question 12
What term applies to a microbe that can synthesize all of its cellular constituents from
a simple carbon source and inorganic salts? Correct Answer
prototroph
Question 13
13) Term that applies to a bacterium that has an absolute requirement of oxygen for
growth.. Correct Answer aerobe
Question 14
14) Term for a bacterium that does not use oxygen for growth, but will grow in the
presence of oxygen. Correct Answer aerotolerant anaerobe
Question 15
The enzyme catalase is used to detoxify which harmful oxygen species?
Correct Answer
hydrogen peroxide
Question 16
16) What is the term for microbes that grow optimally at a pH below 5.5?
Correct Answer acidophiles
, Question 17
17) Term for microbes that grow optimally at very high salt concentrations.
Correct Answer halophiles
Question 18
18) Term for microbes that grow optimally at a pH above 8.5. Correct
Answer alkalophiles
Question 19
19) Term applied to microbes that grow optimally at temperatures below
15°C. Correct Answer psychrophiles
Question 20
20) Enrichment media that contains starch as the sole carbon source is
encouraging the growth of microbes that produce _____ . Correct Answer
the enzyme amylase
Question 21
21) In the environment, one microorganism often provides a nutrient for
another microbe that is unable to synthesize the nutrient by itself. What is
this type of relationship called? Correct Answer syntrophy
Question 22
22) Which of these methods can be used to obtain a viable cell count?
Correct Answer
plate counts of serial dilution
Question 23
23) What would you use to measure the optical density of a bacterial culture?
Correct Answer a spectrophotometer
Question 24
The plate count method was used to estimate the number of bacteria in a culture over
time. Serial dilutions were made of culture samples at various time points. A 0.1 ml portion
of the 10-6 dilution was spread over the surface of a nutrient agar plate for each time
point. The plates were incubated for 24 hrs and the colony forming units (CFU) were
counted on each plate. A total of 45 colonies were counted on the plate for the 30 minute
time point. What was the cell population for this time point?
Correct Answer
d) 45 X 107 CFU/ml