AAB MT Hematology exam with answers

AAB MT Hematology exam with answers
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What is hemoglobin composed of? - CORRECT ANSWERS
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✔✔4 heme, 4 iron, 4 globins
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What disorder does a defect in heme synthesis lead to? -
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CORRECT ANSWERS ✔✔Porphyria |\ |\




Porphyria - CORRECT ANSWERS ✔✔Deficiency in the
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enzymes of the porphyrin pathway leads to insufficient
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production of heme. The principal problem in these
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deficiencies is the accumulation of porphyrins, the heme |\ |\ |\ |\ |\ |\ |\ |\



precursors, which are toxic to tissue in high |\ |\ |\ |\ |\ |\ |\ |\



concentrations.


What is methemoglobin? - CORRECT ANSWERS
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✔✔Methemoglobin is a form of hemoglobin that contains |\ |\ |\ |\ |\ |\ |\ |\



ferric [Fe3+] iron and has a decreased ability to bind
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oxygen. However, the ferric iron has an increased affinity
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for bound oxygen. When methemoglobin concentration is
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elevated in red blood cells, tissue hypoxia can occur.
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What is the function of the enzyme methemoglobin-
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cytochrome C reductase in a red blood cell? - CORRECT |\ |\ |\ |\ |\ |\ |\ |\ |\ |\



ANSWERS ✔✔To keep iron in the ferrous state|\ |\ |\ |\ |\ |\ |\

,What product is formed when ferrous iron of hemoglobin
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is oxidized to the ferric state? - CORRECT ANSWERS
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✔✔Methemoglobin


What is carboxyhemoglobin? - CORRECT ANSWERS ✔✔A
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stable complex of carbon monoxide and hemoglobin that
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forms in RBCs when carbon monoxide is inhaled or
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produced in normal metabolism. Large quantities of it
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hinder delivery of O2 to the body. Tobacco smoking
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(through carbon monoxide inhalation) raises the blood
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levels of COHb by a factor of several times from its
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normal concentrations. |\




Which hemoglobin has a 200 times greater binding
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capacity for the hemoglobin molecule than oxygen? -
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CORRECT ANSWERS ✔✔Carboxyhemoglobin |\ |\




Which hemoglobin derivative causes irreversible changes
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in RBCs? - CORRECT ANSWERS ✔✔Sulfhemoglobin --
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makes blood incapable of carrying oxygen.
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Which of the following hemoglobins is NOT normally
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present in a normal adult? - CORRECT ANSWERS ✔✔Hb-S
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,Which embryonic hemoglobin is normally found in
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newborns? - CORRECT ANSWERS ✔✔Hb-F |\ |\ |\ |\




What is the most abundant hemoglobin in a newborn? -
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CORRECT ANSWERS ✔✔Hb-F |\ |\




What is the molecular structure of Hemoglobin S? -
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CORRECT ANSWERS ✔✔Valine replaces glutamic acid in
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the 6th position of the beta chain
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Which of the following hemoglobins is insoluble under
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lowered oxygen tension? - CORRECT ANSWERS ✔✔Hb-S
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What laboratory test is used to confirm the specific
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diagnosis of sickle cell anemia? - CORRECT ANSWERS
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✔✔Hemoglobin electrophoresis |\




In the solubility test for hemoglobin S, what is the reagent
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responsible for the reduction of the hemoglobin
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molecule? - CORRECT ANSWERS ✔✔Sodium dithionite |\ |\ |\ |\ |\




In hemoglobin C, what replaces glutamic acid? - CORRECT
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ANSWERS ✔✔Lysine
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, Which red blood cell inclusion is characteristic of
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hemoglobin C disease? - CORRECT ANSWERS ✔✔Rod- |\ |\ |\ |\ |\ |\



shaped crystals |\




What is hemoglobin F composed of? - CORRECT ANSWERS
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✔✔2 alpha and 2 gamma chains
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Which hemoglobin is resistant to alkali denaturation? -
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CORRECT ANSWERS ✔✔Hb-F |\ |\




Which hemoglobins are differentiated by the Kleihauer-
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Betke technique? - CORRECT ANSWERS ✔✔Hb-A1 from
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Hb-F


What is the order of migration of hemoglobins, from
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fastest to slowest, on cellulose acetate at pH 8.4? -
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CORRECT ANSWERS ✔✔A1, F, S, C |\ |\ |\ |\ |\




Why is citrate agar used when abnormal hemoglobins are
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identified on cellulose acetate at pH 8.6? - CORRECT
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ANSWERS ✔✔It separates hemoglobin S from hemoglobin
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D
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