Oxford Cambridge and RSA
Monday 12 May 2025 – Morning
AS Level Biology A
H020/01 Breadth in biology
Time allowed: 1 hour 30 minutes
You can use:
* 1 4 4 2 2 3 4 4 5 2 *
• a scientific or graphical calculator
• a ruler (cm/mm)
* H 0 2 0 0 1 *
Please write clearly in black ink. Do not write in the barcodes.
Centre number Candidate number
First name(s)
Last name
INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra space use
the lined pages at the end of this booklet. The question numbers must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working. Marks might be
given for using a correct method, even if your answer is wrong.
INFORMATION
• The total mark for this paper is 70.
• The marks for each question are shown in brackets [ ].
• This document has 28 pages.
ADVICE
• Read each question carefully before you start your answer.
© OCR 2025 [601/4261/3] OCR is an exempt Charity
DC (PQ/CB) 345878/5 Turn over
for more: tyrionpapers.com
, 2
Section A
You should spend a maximum of 25 minutes on this section.
Write your answer for each question in the box provided.
1 Which statement correctly describes microscopes?
A Light microscopes can produce higher resolution images than electron microscopes.
B Scanning electron microscopes produce higher resolution images than transmission electron
microscopes.
C Transmission electron microscopes can achieve higher magnifications than scanning
electron microscopes.
D Transmission electron microscopes can be used to view living tissues.
Your answer [1]
2 Which statement correctly describes phosphorylated nucleotides?
A ADP can be hydrolysed to produce ATP.
B ADP can be phosphorylated to produce ATP.
C All phosphorylated nucleotides have three phosphate groups.
D Energy is released when ATP undergoes a condensation reaction to form ADP and Pi.
Your answer [1]
3 Which type of stem cell can become specialised into more than one cell type but is limited to a
range of cell types?
A Embryonic
B Multipotent
C Pluripotent
D Totipotent
Your answer [1]
© OCR 2025
for more: tyrionpapers.com
, 3
4 A student carries out a non-reducing sugar test on a sample of fruit juice.
What is the correct reason for heating the sample with hydrochloric acid?
A It breaks the ester bond between glucose and fructose
B It breaks the ester bond between glucose and sucrose
C It breaks the glycosidic bond between glucose and fructose
D It breaks the glycosidic bond between glucose and sucrose
Your answer [1]
© OCR 2025 Turn over
for more: tyrionpapers.com
, 4
5 A student is finding out the concentration of a sample of protein.
• They set up five solutions that have a range of different protein concentrations.
• They carry out a chemical test on each solution that produces a colour change.
• They use a colorimeter to find out the absorbance of light through each solution.
The graph shows the calibration curve they plotted for each concentration of protein.
0.20
0.18
0.16
0.14
0.12
Absorbance
0.10
(arbitrary units)
0.08
0.06
0.04
0.02
0.00
0.0 0.5 1.0 1.5 2.0 2.5
Percentage concentration of protein
They carry out the same chemical test on the sample of protein with the unknown concentration
and measure the absorbance as 0.16 arbitrary units.
Which statement correctly describes the results of the experiment?
A The concentration of protein in the sample tested is 1.6%.
B The concentration of protein in the sample tested is too low to read from the graph.
C The greater the concentration of protein, the less light is absorbed by the sample.
D The greater the concentration of protein, the less light is transmitted through the sample.
Your answer [1]
© OCR 2025
for more: tyrionpapers.com
Monday 12 May 2025 – Morning
AS Level Biology A
H020/01 Breadth in biology
Time allowed: 1 hour 30 minutes
You can use:
* 1 4 4 2 2 3 4 4 5 2 *
• a scientific or graphical calculator
• a ruler (cm/mm)
* H 0 2 0 0 1 *
Please write clearly in black ink. Do not write in the barcodes.
Centre number Candidate number
First name(s)
Last name
INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra space use
the lined pages at the end of this booklet. The question numbers must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working. Marks might be
given for using a correct method, even if your answer is wrong.
INFORMATION
• The total mark for this paper is 70.
• The marks for each question are shown in brackets [ ].
• This document has 28 pages.
ADVICE
• Read each question carefully before you start your answer.
© OCR 2025 [601/4261/3] OCR is an exempt Charity
DC (PQ/CB) 345878/5 Turn over
for more: tyrionpapers.com
, 2
Section A
You should spend a maximum of 25 minutes on this section.
Write your answer for each question in the box provided.
1 Which statement correctly describes microscopes?
A Light microscopes can produce higher resolution images than electron microscopes.
B Scanning electron microscopes produce higher resolution images than transmission electron
microscopes.
C Transmission electron microscopes can achieve higher magnifications than scanning
electron microscopes.
D Transmission electron microscopes can be used to view living tissues.
Your answer [1]
2 Which statement correctly describes phosphorylated nucleotides?
A ADP can be hydrolysed to produce ATP.
B ADP can be phosphorylated to produce ATP.
C All phosphorylated nucleotides have three phosphate groups.
D Energy is released when ATP undergoes a condensation reaction to form ADP and Pi.
Your answer [1]
3 Which type of stem cell can become specialised into more than one cell type but is limited to a
range of cell types?
A Embryonic
B Multipotent
C Pluripotent
D Totipotent
Your answer [1]
© OCR 2025
for more: tyrionpapers.com
, 3
4 A student carries out a non-reducing sugar test on a sample of fruit juice.
What is the correct reason for heating the sample with hydrochloric acid?
A It breaks the ester bond between glucose and fructose
B It breaks the ester bond between glucose and sucrose
C It breaks the glycosidic bond between glucose and fructose
D It breaks the glycosidic bond between glucose and sucrose
Your answer [1]
© OCR 2025 Turn over
for more: tyrionpapers.com
, 4
5 A student is finding out the concentration of a sample of protein.
• They set up five solutions that have a range of different protein concentrations.
• They carry out a chemical test on each solution that produces a colour change.
• They use a colorimeter to find out the absorbance of light through each solution.
The graph shows the calibration curve they plotted for each concentration of protein.
0.20
0.18
0.16
0.14
0.12
Absorbance
0.10
(arbitrary units)
0.08
0.06
0.04
0.02
0.00
0.0 0.5 1.0 1.5 2.0 2.5
Percentage concentration of protein
They carry out the same chemical test on the sample of protein with the unknown concentration
and measure the absorbance as 0.16 arbitrary units.
Which statement correctly describes the results of the experiment?
A The concentration of protein in the sample tested is 1.6%.
B The concentration of protein in the sample tested is too low to read from the graph.
C The greater the concentration of protein, the less light is absorbed by the sample.
D The greater the concentration of protein, the less light is transmitted through the sample.
Your answer [1]
© OCR 2025
for more: tyrionpapers.com
