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BCHM 4360 Exam 3 Study Guide 2025 – Biochemistry
Concepts, Practice Questions & Answer Key
Prepare for success in BCHM 4360 Exam 3 with this complete 2025 biochemistry study guide,
featuring metabolism pathways, enzyme kinetics, molecular regulation, and detailed
practice questions with answer explanations. Perfect for university-level biochemistry students.
BCHM 4360 Exam 3,
BCHM 4360 study guide,
BCHM 4360 biochemistry exam,
BCHM 4360 Exam 3 answers,
This group of introns can also act as mobile genetic elements by reverse splicing, and they often have
open reading frames within the introns that assist splicing - ANSWER-Group II introns
_________________ genes often have several introns which can be very long (thousands of bases) and
account for up to 90% of a pre-mRNA - ANSWER-Eukaryotic
The human _________________ gene has 78 introns and exons are sometimes many thousands of
bases apart - ANSWER-Dystrophin
Some eukaryotes, like ______________, have fewer, shorter introns - ANSWER-Yeast
Most eukaryotic introns are not - ANSWER-Self-splicing
,2|Page
Most eukaryotic introns are not self splicing, so splicing is mediated by the - ANSWER-Spliceosome
Spliceosome is made up of several small - ANSWER-Small nuclear ribonucleoproteins (snRNP)
Spliceosome catalyzed splicing is similar to that of this group of introns - ANSWER-Group II introns
Spliceosomes recognize ________________ in pre-mRNA - ANSWER-Splice sites
Splice sites are defined by
5' and 3' splice sites
Branch point nucleotide within the intron
Polypyrimidine region before the 3' splice site - ANSWER-Short sequence motifs
Usually, introns have ________ and ________ at their ends - ANSWER-GU and AG
The spliceosome has _________ snRNPs, which are - ANSWER-5, U1, U2, U4, U5, U6
The spliceosome has 5 snRNPs (U1, U2, U4, U5, U6). Each has an ____________ of about 100-300
nucleotides, plus proteins - ANSWER-snRNA
The snRNAs of the snRNPS of the spliceosome form base pairs with the pre-mRNA and work as the
_________________ part of the snRNP - ANSWER-Recognition
Splicing by spliceosome in eukaryotes
Step 1 - The 5' splice site is recognized by ____________ and other sequences are bound by non-snRNPs
- ANSWER-U1
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Splicing by spliceosome in eukaryotes
Steps 2 and 3 - The rest of the splicing apparatus binds, sometimes displacing other factors, including
the ___________________ - ANSWER-Branch point binding protein (BBP)
Splicing by spliceosomes in eukaryotes
Step 4 - The pre-mRNA rearranges. The first transesterification occurs and the _____________ forms -
ANSWER-Lariat
Splicing by spliceosomes in eukaryotes
Step 5 - Other rearrangements then bring the two exons for the second ___________________ -
ANSWER-Transesterification
Left at splice junctions after splicing
Marks the transcript as processed and interacts with export and translation proteins - ANSWER-Exon
junction complex (EJC)
Where exons in the same molecule are joined together - ANSWER-Cis splicing
Joins exons from different RNA molecules
Unusual - ANSWER-Trans splicing
In trans splicing, a short RNA called ____________________ is joined to different mRNAs - ANSWER-
Spliced leader RNA (SL RNA)
In trans splicing, spliced leader RNA (SL RNA) replaces the ___________ snRNP and interacts with other
snRNPs at the 3' splice site - ANSWER-U1
BCHM 4360 Exam 3 Study Guide 2025 – Biochemistry
Concepts, Practice Questions & Answer Key
Prepare for success in BCHM 4360 Exam 3 with this complete 2025 biochemistry study guide,
featuring metabolism pathways, enzyme kinetics, molecular regulation, and detailed
practice questions with answer explanations. Perfect for university-level biochemistry students.
BCHM 4360 Exam 3,
BCHM 4360 study guide,
BCHM 4360 biochemistry exam,
BCHM 4360 Exam 3 answers,
This group of introns can also act as mobile genetic elements by reverse splicing, and they often have
open reading frames within the introns that assist splicing - ANSWER-Group II introns
_________________ genes often have several introns which can be very long (thousands of bases) and
account for up to 90% of a pre-mRNA - ANSWER-Eukaryotic
The human _________________ gene has 78 introns and exons are sometimes many thousands of
bases apart - ANSWER-Dystrophin
Some eukaryotes, like ______________, have fewer, shorter introns - ANSWER-Yeast
Most eukaryotic introns are not - ANSWER-Self-splicing
,2|Page
Most eukaryotic introns are not self splicing, so splicing is mediated by the - ANSWER-Spliceosome
Spliceosome is made up of several small - ANSWER-Small nuclear ribonucleoproteins (snRNP)
Spliceosome catalyzed splicing is similar to that of this group of introns - ANSWER-Group II introns
Spliceosomes recognize ________________ in pre-mRNA - ANSWER-Splice sites
Splice sites are defined by
5' and 3' splice sites
Branch point nucleotide within the intron
Polypyrimidine region before the 3' splice site - ANSWER-Short sequence motifs
Usually, introns have ________ and ________ at their ends - ANSWER-GU and AG
The spliceosome has _________ snRNPs, which are - ANSWER-5, U1, U2, U4, U5, U6
The spliceosome has 5 snRNPs (U1, U2, U4, U5, U6). Each has an ____________ of about 100-300
nucleotides, plus proteins - ANSWER-snRNA
The snRNAs of the snRNPS of the spliceosome form base pairs with the pre-mRNA and work as the
_________________ part of the snRNP - ANSWER-Recognition
Splicing by spliceosome in eukaryotes
Step 1 - The 5' splice site is recognized by ____________ and other sequences are bound by non-snRNPs
- ANSWER-U1
, 3|Page
Splicing by spliceosome in eukaryotes
Steps 2 and 3 - The rest of the splicing apparatus binds, sometimes displacing other factors, including
the ___________________ - ANSWER-Branch point binding protein (BBP)
Splicing by spliceosomes in eukaryotes
Step 4 - The pre-mRNA rearranges. The first transesterification occurs and the _____________ forms -
ANSWER-Lariat
Splicing by spliceosomes in eukaryotes
Step 5 - Other rearrangements then bring the two exons for the second ___________________ -
ANSWER-Transesterification
Left at splice junctions after splicing
Marks the transcript as processed and interacts with export and translation proteins - ANSWER-Exon
junction complex (EJC)
Where exons in the same molecule are joined together - ANSWER-Cis splicing
Joins exons from different RNA molecules
Unusual - ANSWER-Trans splicing
In trans splicing, a short RNA called ____________________ is joined to different mRNAs - ANSWER-
Spliced leader RNA (SL RNA)
In trans splicing, spliced leader RNA (SL RNA) replaces the ___________ snRNP and interacts with other
snRNPs at the 3' splice site - ANSWER-U1
