Kaat Mols, 3BA BIO VUB S2
An immunogen is composed of T and B cell epitopes
Non-immunogenic antigen. = only B-cell epitopes
Immunogen: B cell epitopes + T cell epitopes (eg subunit vaccines)
Take home messages: Immunogenicity vs antigenicity
- Ag is not necessarily an immunogen
- Immunogen is recognized by T cells (T cell epitopes) and B cells (B cell epitopes)
- Optimal subunit vaccines are composed of B en T cell epitopes
Hormonal vs cellular immunity
,Kaat Mols, 3BA BIO VUB S2
LES 4: Cellular immunity
Delayed Type hypersensitivity (DTH) is mediated by macrophages with the help of CD4+
Th1 cells • CD8+ cytotoxic T cells (CTL) mediate viral protection and graft rejection with the
help of CD4+ Th1 cells (in vitro MLC and CML)
Humorale vs cellulaire immuniteit
,Kaat Mols, 3BA BIO VUB S2
Intracellular pathogens
Delayed type hypersensitivity (Tdth)
4 types of hypersensitivity reactions
1-3: HI- mediated hypersensitivity 4: CI mediatd hypersensitivity
Type 1 Type 2 Type 3 Type 4
Mast cells vind IgE Antibody is directed Immune complexes Antigen-sensitized T
via their Fc against antigens on an are deposited in the cells release
receptors. On individual’s own clls tissue. Complement lympphokines
encountering antigen (target cell). This can is activated an following a
the IgE becomes lead to cytotoxic polymorohs are secondary contact
crosslinked, action by K cells or attracted to the site with the same
inducing complement-mediated of deposition, antigen.
defranulation and lysis causing local Lymphokines induce
release of mediators damage inflammatory
reactions and
activate and attract
macrophages wich
release mediators
Allergies Medication-induced Glomerulonephritis Delayed type
hemolytic anemia (eg during SLE hypersensitivity (><
cephalosporin immediate type
antibiotics) hypersensitivity
Anti Rh+ reaciont such as allergy
, Kaat Mols, 3BA BIO VUB S2
DTH reactions are cell-mediated => CI
Ag wortd bij muis ingespoten.
Dag 8: serum afgnomen => transfer nr muis en
Ag => 24 h wachten er is bijna geen Tdth
respons
Dag 15
- Ag wordt in muis ingespoten (locaal) en
24 gewacht => +++ sterke respons (eg
tbc skin test)
- Spleen cellen trasgfer nr muis en ag
ingespoten => sterke respons => dus
heti s cellulaire immuniteit (memory:
cells, reaction: cells)
An immunogen is composed of T and B cell epitopes
Non-immunogenic antigen. = only B-cell epitopes
Immunogen: B cell epitopes + T cell epitopes (eg subunit vaccines)
Take home messages: Immunogenicity vs antigenicity
- Ag is not necessarily an immunogen
- Immunogen is recognized by T cells (T cell epitopes) and B cells (B cell epitopes)
- Optimal subunit vaccines are composed of B en T cell epitopes
Hormonal vs cellular immunity
,Kaat Mols, 3BA BIO VUB S2
LES 4: Cellular immunity
Delayed Type hypersensitivity (DTH) is mediated by macrophages with the help of CD4+
Th1 cells • CD8+ cytotoxic T cells (CTL) mediate viral protection and graft rejection with the
help of CD4+ Th1 cells (in vitro MLC and CML)
Humorale vs cellulaire immuniteit
,Kaat Mols, 3BA BIO VUB S2
Intracellular pathogens
Delayed type hypersensitivity (Tdth)
4 types of hypersensitivity reactions
1-3: HI- mediated hypersensitivity 4: CI mediatd hypersensitivity
Type 1 Type 2 Type 3 Type 4
Mast cells vind IgE Antibody is directed Immune complexes Antigen-sensitized T
via their Fc against antigens on an are deposited in the cells release
receptors. On individual’s own clls tissue. Complement lympphokines
encountering antigen (target cell). This can is activated an following a
the IgE becomes lead to cytotoxic polymorohs are secondary contact
crosslinked, action by K cells or attracted to the site with the same
inducing complement-mediated of deposition, antigen.
defranulation and lysis causing local Lymphokines induce
release of mediators damage inflammatory
reactions and
activate and attract
macrophages wich
release mediators
Allergies Medication-induced Glomerulonephritis Delayed type
hemolytic anemia (eg during SLE hypersensitivity (><
cephalosporin immediate type
antibiotics) hypersensitivity
Anti Rh+ reaciont such as allergy
, Kaat Mols, 3BA BIO VUB S2
DTH reactions are cell-mediated => CI
Ag wortd bij muis ingespoten.
Dag 8: serum afgnomen => transfer nr muis en
Ag => 24 h wachten er is bijna geen Tdth
respons
Dag 15
- Ag wordt in muis ingespoten (locaal) en
24 gewacht => +++ sterke respons (eg
tbc skin test)
- Spleen cellen trasgfer nr muis en ag
ingespoten => sterke respons => dus
heti s cellulaire immuniteit (memory:
cells, reaction: cells)
