AP Biology Lab 3.1-3.3: Enzyme
Structure & Function Correct Answers
A student designs an experiment to investigate the effect of environmental pHpH on
amylase function. The design of the experiment is presented in Table 1.
Which of the following statements best justifies the inclusion of test tube as a control
in the experiment? - ANSWER-It will show the color change that occurs in the
absence of enzyme activity.
A researcher claims that some bacteria contain factors that influence the function of
a particular enzyme but other bacteria do not. To test the claim experimentally, the
researcher will grow two different bacterial strains in separate liquid cultures and
isolate the contents of the cells in each culture. The researcher will add different
combinations of cellular contents, substrate, and enzyme to test tubes containing a
buffer solution adjusted to the optimal pH of the enzyme and then measure the rate
of product formation. The design of the researcher's experiment is presented in
Table 1.
Which of the following statements best justifies the inclusion of test tubes 3 and 7 in
the experiment? - ANSWER-They will show whether the isolated cellular contents
have enzymatic activity.
For the following questions: Graphs I-IV depict the effect of pH on the activity of four
different hydrolytic enzymes. - ANSWER-II only (it is the graph that increases then
goes "flat")
Gelatin is a protein that is derived from collagen which is found in the bones, skin,
and connective tissue of animals. To investigate the ability of various enzymes to
digest gelatin, a group of students set up an assay involving camera film. Camera
film contains gelatin and appears black when exposed to light but turns clear as the
gelatin gets broken down. The students incubated pieces of exposed camera film in
test tubes, each containing one of three different enzyme solutions (trypsin, lipase, or
amylase) as indicated in Figure 1. The students recorded the time it took for the
enzymes to digest the gelatin in each test tube, turning the film from black to clear.
Which of the following would be the most appropriate control for this experiment? -
ANSWER-A test tube containing a piece of exposed camera film submerged in water
For following group of questions first study the description of the data and then
choose the one best answer to each question following it and fill in the corresponding
oval on the answer sheet.
To study the actions of the enzyme catalase on hydrogen peroxide, students
performed the following experiment. Catalase was extracted from potatoes by
blending raw potatoes in a blender with cold distilled water. The filtrate was stored on
ice. The following hydrogen peroxide solutions were made: 1 percent, 5 percent, 10
Structure & Function Correct Answers
A student designs an experiment to investigate the effect of environmental pHpH on
amylase function. The design of the experiment is presented in Table 1.
Which of the following statements best justifies the inclusion of test tube as a control
in the experiment? - ANSWER-It will show the color change that occurs in the
absence of enzyme activity.
A researcher claims that some bacteria contain factors that influence the function of
a particular enzyme but other bacteria do not. To test the claim experimentally, the
researcher will grow two different bacterial strains in separate liquid cultures and
isolate the contents of the cells in each culture. The researcher will add different
combinations of cellular contents, substrate, and enzyme to test tubes containing a
buffer solution adjusted to the optimal pH of the enzyme and then measure the rate
of product formation. The design of the researcher's experiment is presented in
Table 1.
Which of the following statements best justifies the inclusion of test tubes 3 and 7 in
the experiment? - ANSWER-They will show whether the isolated cellular contents
have enzymatic activity.
For the following questions: Graphs I-IV depict the effect of pH on the activity of four
different hydrolytic enzymes. - ANSWER-II only (it is the graph that increases then
goes "flat")
Gelatin is a protein that is derived from collagen which is found in the bones, skin,
and connective tissue of animals. To investigate the ability of various enzymes to
digest gelatin, a group of students set up an assay involving camera film. Camera
film contains gelatin and appears black when exposed to light but turns clear as the
gelatin gets broken down. The students incubated pieces of exposed camera film in
test tubes, each containing one of three different enzyme solutions (trypsin, lipase, or
amylase) as indicated in Figure 1. The students recorded the time it took for the
enzymes to digest the gelatin in each test tube, turning the film from black to clear.
Which of the following would be the most appropriate control for this experiment? -
ANSWER-A test tube containing a piece of exposed camera film submerged in water
For following group of questions first study the description of the data and then
choose the one best answer to each question following it and fill in the corresponding
oval on the answer sheet.
To study the actions of the enzyme catalase on hydrogen peroxide, students
performed the following experiment. Catalase was extracted from potatoes by
blending raw potatoes in a blender with cold distilled water. The filtrate was stored on
ice. The following hydrogen peroxide solutions were made: 1 percent, 5 percent, 10
