https://www.stuvia.com/
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Chapter 1 RBCs and Platelet Preservation: Historical Perspectives and Current Trends
jl jl jl jl jl jl jl jl jl jl
Multiple Choicejl
Identify the choice that best completes the statement or answers the question.
jl jl jl jl jl jl jl jl jl jl jl
1. Which metabolic pathway is responsible for generating 90% of the ATP for the RBC?
jl jl jl jl jl jl jl jl jl jl jl jl jl
a. Pentose phosphate shunt c. Glycolysis jl jl
b. Luebering-Rapoport shunt d. Methemoglobin reductase jl j l jl
2. A unit of blood was returned to the blood bank before it was spiked. Apparently the patient’s IV failed. The uni
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
t of blood was outside the blood bank for 35 minutes. Which of the statements below is most accurate?
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
a. The unit of blood should be discarded immediately. jl jl jl jl jl jl jl
b. The unit of blood can be returned to inventory. jl jl jl jl jl jl jl jl
c. The unit of blood must be transfused within 4 hours or be discarded at the end of that time.
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
d. The unit of blood must be transfused with 24 hours. jl jl jl jl jl jl jl jl jl
3. What is the average diameter of a platelet? jl jl jl jl jl jl jl
a. 5 to 10 µm jl jl jl c. 1 to 2 µm jl jl jl
b. 2 to 4 µm jl jl jl d. 4 to 6 µm
j l j l jl jl jl
4. In the normal hemoglobin-
jl jl jl
oxygen dissociation curve, what percentage of oxygen is released to the tissues when PO2 averages 40 m
jl jl jl jl jl jl jl jl jl jl jl jl jl j l jl jl
m Hg? jl
a. 75% c. 100%
b. 25% d. 50% j l
5. What factors are known to influence platelet metabolism and function?
jl jl jl jl jl jl jl jl jl
a. Storage temperature c. Platelet count jl jl
b. Initial pH d. All of the above jl j l jl jl jl
6. Which of the following red blood cell morphologies may be present on the peripheral blood smear as a result of
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
loss of RBC membrane? jl jl jl
a. Spherocytes c. Burr cells jl
b. Target cells d. Schistocytes jl j l
7. What does the term autologous transfusion refer to?
jl jl jl jl jl jl jl
a. A parent donating blood for his or her child jl jl jl jl jl jl jl jl
b. An individual donating blood for a friend jl jl jl jl jl jl
c. An individual donating blood for a relative jl jl jl jl jl jl
d. An individual donating blood for his or her own transfusion
jl jl jl jl jl jl jl jl jl
8. What is the primary function of hemoglobin? jl jl jl jl jl jl
a. Iron metabolism jl c. Oxygen transport jl
b. Porphyrin synthesis jl d. Signal transduction
j l jl
9. All of the following areas of red blood cell biology are crucial for normal erythrocyte survival except:
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
a. Cellular metabolism. c. Site of the ABO antigen attachment. jl jl jl jl jl jl
b. RBC membrane. d. Hemoglobin structure.jl j l jl
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j l 10. What is the correct biochemical composition of the RBC membrane?
j l jl jl jl jl jl jl jl jl jl
a. 52% protein, 40% lipid, 8% carbohydrate jl jl jl jl jl
b. 40% protein, 8% lipid, 52% carbohydrate jl jl jl jl jl
c. 8% protein, 52% lipid, 40% carbohydrate
jl jl jl jl jl
d. 8% lipid, 40% carbohydrate, 52% protein
jl jl jl jl jl
j l 11. All of the following biochemical changes are associated with loss of red blood cell viability upon storage
j l jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
except:
a. Decreased pH. c. Increased ATP level. jl jl jl
b. Loss of red blood cell function. d. Decreased glucose consumption.
jl jl jl jl jl j l jl jl
j l 12. Which red blood cell preservative has a storage time of 35 days?
j l jl jl jl jl jl jl jl jl jl jl jl
a. ACD c. AS-1
b. CPDA-1 d. CPD j l j l
j l 13. The RBC membrane is relatively permeable to all of the following except:
j l jl jl jl jl jl jl jl jl jl jl jl
a. Chloride. c. Bicarbonate.
b. Sodium. d. Water. j l
jl 14. What percentage of platelets is sequestered in the spleen as functional reserve after being released from the b
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
one marrow? jl
a. 30% c. 10%
b. 50% d. 25% j l j l
j l 15. What is the major biochemical consideration in platelet storage?
j l jl jl jl jl jl jl jl jl
a. Glucose metabolism c. Production of carbon dioxide jl jl jl jl
b. Oxygen supply d. Regulation of pH jl j l jl jl
jl 16. What would the hemoglobin-
jl jl jl jl
oxygen dissociation curve depict in a patient exhibiting clinical signs of alkalosis?
jl jl jl jl jl jl jl jl jl jl jl
a. Normal c. Shift to the right jl jl jl
b. Shift to the left d. None of the above
jl jl jl j l jl jl jl
j l 17. Name the main lipid components of a red blood cell membrane.
j l jl jl jl jl jl jl jl jl jl jl
a. Phospholipid c. Glycolipid
b. Sphingomyelin d. Glycophorin A j l jl
j l 18. The ABO blood groups were discovered in 1901 by whom?
j l jl jl jl jl jl jl jl jl jl
a. Charles Drew c. Loutit and Mollison jl jl jl
b. Karl Landsteiner d. Edward Lindeman
jl j l jl
j l 19. A standing order of platelets was shipped to your facility by your supplier. It was inadvertently left in the corn
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
er of the department until discovered 36 hours later. What would the appropriate action be for the blood banker
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
?
a. If the temperature in the box was 22 +/-
jl jl jl jl jl jl jl jl
2°C and the platelet swirl seemed OK, it would be OK to accept the unit into inventory.
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
b. The platelets have fallen outside the supplier’s quality assurance. The unit should be disc
jl jl jl jl jl jl jl jl jl jl jl jl jl
arded because the pH has probably dropped too low and platelet activation has been compr
jl jl jl jl jl jl jl jl jl jl jl jl jl jl
omised.
c. If the temperature was 1°C to 6°C and the platelet swirl seemed OK, it would be OK to
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
Thisjlstudyjlsourcejlwasjldownloadedjlbyjl100000826342158jlfromjlCourseHero.comjlonjl09-15-2021jl17:54:15jlGMTjl-05:00
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,https://www.stuvia.com/
accept the unit into inventory. jl jl jl jl
d. If the platelets appeared OK and passed the platelet swirl test after being placed on the ag
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
itator, they could be accepted into the inventory.
jl jl jl jl jl jl jl
jl 20. Which metabolic pathway permits the accumulation of 2,3 diphosphoglycerate (2,3-DPG)?
j l jl jl jl jl jl jl jl jl jl
a. Glycolysis c. Pentose phosphate shunt jl jl
b. Luebering-Rapoport shunt d. Methemoglobin reductase jl j l jl
jl 21. All of the following are consistent with a "shift to the right" of the hemoglobin-oxygen dissociation curve
j l jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
except:
a. Increased 2,3-DPG. jl
b. 50% O2 saturation to tissues. jl jl jl jl
c. Decreased 2,3-DPG. jl
d. Decreased hemoglobin affinity for O2. jl jl jl jl
j l 22. What is the normal platelet count range per cubic millimeter?
j l jl jl jl jl jl jl jl jl jl
a. 100 to 350 jl c. 50,000 to 200,000
jl jl jl
b. 150,000 to 350,000 d. 350,000 to 500,000 jl jl j l jl jl
j l 23. What cryoprotective agent is added to red blood cells upon freezing?
j l jl jl jl jl jl jl jl jl jl jl
a. Dextrose c. Glycerol
b. Adsol d. All of the above j l jl jl jl
jl 24. If platelets are to be stored for 5 days on a rotator, what is the optimal storage temperature?
j l jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
a. 1°C to 6°C jl c. 35°C to 37°C
jl jl jl
b. 20°C to 24°C jl d. 1°C to 10°Cjl j l jl jl
j l 25. What role do platelets play in hemostasis?
j l jl jl jl jl jl jl
a. Maintenance of vascular integrity jl jl jl
b. Initial arrest of bleeding by platelet plug formation
jl jl jl jl jl jl jl
c. Stabilization of the hemostatic plug jl jl jl jl
d. All of the above jl jl jl
j l 26. Which of the following best describes "integral" membrane proteins?
j l jl jl jl jl jl jl jl jl
a. Reside at the cytoplasmic surface of membrane jl jl jl jl jl jl
b. Span the entire membrane surface jl jl jl jl
c. Form the red blood cell cytoskeleton jl jl jl jl jl
d. None of the above jl jl jl
j l 27. How is stroma-free hemoglobin solution prepared?
j l jl jl jl jl jl
a. Outdated red blood cells are concentrated, and stroma is removed. jl jl jl jl jl jl jl jl jl
b. Outdated red blood cells are diluted with saline, and stroma is removed. jl jl jl jl jl jl jl jl jl jl jl
c. Outdated red blood cells are lysed, and stroma is removed. jl jl jl jl jl jl jl jl jl
d. None of the above jl jl jl
j l 28. What is the normal life span of an RBC?
j l jl jl jl jl jl jl jl jl
a. 100 days jl c. 120 hours jl
b. 120 days jl d. 2 days j l jl
jl 29. Regarding loss of RBC membrane deformability, all of the following are true except:
j l jl jl jl jl jl jl jl jl jl jl jl jl
a. Increase in ATP level. jl jl jl
b. Decrease in ATP level. jl jl jl
Thisjlstudyjlsourcejlwasjldownloadedjlbyjl100000826342158jlfromjlCourseHero.comjlonjl09-15-2021jl17:54:15jlGMTjl-05:00
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,https://www.stuvia.com/
Chapter 1 RBCs and Platelet Preservation: Historical Perspectives and Current Trends
jl jl jl jl jl jl jl jl jl jl
Multiple Choicejl
Identify the choice that best completes the statement or answers the question.
jl jl jl jl jl jl jl jl jl jl jl
1. Which metabolic pathway is responsible for generating 90% of the ATP for the RBC?
jl jl jl jl jl jl jl jl jl jl jl jl jl
a. Pentose phosphate shunt c. Glycolysis jl jl
b. Luebering-Rapoport shunt d. Methemoglobin reductase jl j l jl
2. A unit of blood was returned to the blood bank before it was spiked. Apparently the patient’s IV failed. The uni
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
t of blood was outside the blood bank for 35 minutes. Which of the statements below is most accurate?
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
a. The unit of blood should be discarded immediately. jl jl jl jl jl jl jl
b. The unit of blood can be returned to inventory. jl jl jl jl jl jl jl jl
c. The unit of blood must be transfused within 4 hours or be discarded at the end of that time.
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
d. The unit of blood must be transfused with 24 hours. jl jl jl jl jl jl jl jl jl
3. What is the average diameter of a platelet? jl jl jl jl jl jl jl
a. 5 to 10 µm jl jl jl c. 1 to 2 µm jl jl jl
b. 2 to 4 µm jl jl jl d. 4 to 6 µm
j l j l jl jl jl
4. In the normal hemoglobin-
jl jl jl
oxygen dissociation curve, what percentage of oxygen is released to the tissues when PO2 averages 40 m
jl jl jl jl jl jl jl jl jl jl jl jl jl j l jl jl
m Hg? jl
a. 75% c. 100%
b. 25% d. 50% j l
5. What factors are known to influence platelet metabolism and function?
jl jl jl jl jl jl jl jl jl
a. Storage temperature c. Platelet count jl jl
b. Initial pH d. All of the above jl j l jl jl jl
6. Which of the following red blood cell morphologies may be present on the peripheral blood smear as a result of
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
loss of RBC membrane? jl jl jl
a. Spherocytes c. Burr cells jl
b. Target cells d. Schistocytes jl j l
7. What does the term autologous transfusion refer to?
jl jl jl jl jl jl jl
a. A parent donating blood for his or her child jl jl jl jl jl jl jl jl
b. An individual donating blood for a friend jl jl jl jl jl jl
c. An individual donating blood for a relative jl jl jl jl jl jl
d. An individual donating blood for his or her own transfusion
jl jl jl jl jl jl jl jl jl
8. What is the primary function of hemoglobin? jl jl jl jl jl jl
a. Iron metabolism jl c. Oxygen transport jl
b. Porphyrin synthesis jl d. Signal transduction
j l jl
9. All of the following areas of red blood cell biology are crucial for normal erythrocyte survival except:
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
a. Cellular metabolism. c. Site of the ABO antigen attachment. jl jl jl jl jl jl
b. RBC membrane. d. Hemoglobin structure.jl j l jl
Thisjlstudyjlsourcejlwasjldownloadedjlbyjl100000826342158jlfromjlCourseHero.comjlonjl09-15-2021jl17:54:15jlGMTjl-05:00
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,https://www.stuvia.com/
j l 10. What is the correct biochemical composition of the RBC membrane?
j l jl jl jl jl jl jl jl jl jl
a. 52% protein, 40% lipid, 8% carbohydrate jl jl jl jl jl
b. 40% protein, 8% lipid, 52% carbohydrate jl jl jl jl jl
c. 8% protein, 52% lipid, 40% carbohydrate
jl jl jl jl jl
d. 8% lipid, 40% carbohydrate, 52% protein
jl jl jl jl jl
j l 11. All of the following biochemical changes are associated with loss of red blood cell viability upon storage
j l jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
except:
a. Decreased pH. c. Increased ATP level. jl jl jl
b. Loss of red blood cell function. d. Decreased glucose consumption.
jl jl jl jl jl j l jl jl
j l 12. Which red blood cell preservative has a storage time of 35 days?
j l jl jl jl jl jl jl jl jl jl jl jl
a. ACD c. AS-1
b. CPDA-1 d. CPD j l j l
j l 13. The RBC membrane is relatively permeable to all of the following except:
j l jl jl jl jl jl jl jl jl jl jl jl
a. Chloride. c. Bicarbonate.
b. Sodium. d. Water. j l
jl 14. What percentage of platelets is sequestered in the spleen as functional reserve after being released from the b
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
one marrow? jl
a. 30% c. 10%
b. 50% d. 25% j l j l
j l 15. What is the major biochemical consideration in platelet storage?
j l jl jl jl jl jl jl jl jl
a. Glucose metabolism c. Production of carbon dioxide jl jl jl jl
b. Oxygen supply d. Regulation of pH jl j l jl jl
jl 16. What would the hemoglobin-
jl jl jl jl
oxygen dissociation curve depict in a patient exhibiting clinical signs of alkalosis?
jl jl jl jl jl jl jl jl jl jl jl
a. Normal c. Shift to the right jl jl jl
b. Shift to the left d. None of the above
jl jl jl j l jl jl jl
j l 17. Name the main lipid components of a red blood cell membrane.
j l jl jl jl jl jl jl jl jl jl jl
a. Phospholipid c. Glycolipid
b. Sphingomyelin d. Glycophorin A j l jl
j l 18. The ABO blood groups were discovered in 1901 by whom?
j l jl jl jl jl jl jl jl jl jl
a. Charles Drew c. Loutit and Mollison jl jl jl
b. Karl Landsteiner d. Edward Lindeman
jl j l jl
j l 19. A standing order of platelets was shipped to your facility by your supplier. It was inadvertently left in the corn
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
er of the department until discovered 36 hours later. What would the appropriate action be for the blood banker
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
?
a. If the temperature in the box was 22 +/-
jl jl jl jl jl jl jl jl
2°C and the platelet swirl seemed OK, it would be OK to accept the unit into inventory.
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
b. The platelets have fallen outside the supplier’s quality assurance. The unit should be disc
jl jl jl jl jl jl jl jl jl jl jl jl jl
arded because the pH has probably dropped too low and platelet activation has been compr
jl jl jl jl jl jl jl jl jl jl jl jl jl jl
omised.
c. If the temperature was 1°C to 6°C and the platelet swirl seemed OK, it would be OK to
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
Thisjlstudyjlsourcejlwasjldownloadedjlbyjl100000826342158jlfromjlCourseHero.comjlonjl09-15-2021jl17:54:15jlGMTjl-05:00
https://www.coursehero.com/file/25537437/ch01rtf/
,https://www.stuvia.com/
accept the unit into inventory. jl jl jl jl
d. If the platelets appeared OK and passed the platelet swirl test after being placed on the ag
jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
itator, they could be accepted into the inventory.
jl jl jl jl jl jl jl
jl 20. Which metabolic pathway permits the accumulation of 2,3 diphosphoglycerate (2,3-DPG)?
j l jl jl jl jl jl jl jl jl jl
a. Glycolysis c. Pentose phosphate shunt jl jl
b. Luebering-Rapoport shunt d. Methemoglobin reductase jl j l jl
jl 21. All of the following are consistent with a "shift to the right" of the hemoglobin-oxygen dissociation curve
j l jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
except:
a. Increased 2,3-DPG. jl
b. 50% O2 saturation to tissues. jl jl jl jl
c. Decreased 2,3-DPG. jl
d. Decreased hemoglobin affinity for O2. jl jl jl jl
j l 22. What is the normal platelet count range per cubic millimeter?
j l jl jl jl jl jl jl jl jl jl
a. 100 to 350 jl c. 50,000 to 200,000
jl jl jl
b. 150,000 to 350,000 d. 350,000 to 500,000 jl jl j l jl jl
j l 23. What cryoprotective agent is added to red blood cells upon freezing?
j l jl jl jl jl jl jl jl jl jl jl
a. Dextrose c. Glycerol
b. Adsol d. All of the above j l jl jl jl
jl 24. If platelets are to be stored for 5 days on a rotator, what is the optimal storage temperature?
j l jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl jl
a. 1°C to 6°C jl c. 35°C to 37°C
jl jl jl
b. 20°C to 24°C jl d. 1°C to 10°Cjl j l jl jl
j l 25. What role do platelets play in hemostasis?
j l jl jl jl jl jl jl
a. Maintenance of vascular integrity jl jl jl
b. Initial arrest of bleeding by platelet plug formation
jl jl jl jl jl jl jl
c. Stabilization of the hemostatic plug jl jl jl jl
d. All of the above jl jl jl
j l 26. Which of the following best describes "integral" membrane proteins?
j l jl jl jl jl jl jl jl jl
a. Reside at the cytoplasmic surface of membrane jl jl jl jl jl jl
b. Span the entire membrane surface jl jl jl jl
c. Form the red blood cell cytoskeleton jl jl jl jl jl
d. None of the above jl jl jl
j l 27. How is stroma-free hemoglobin solution prepared?
j l jl jl jl jl jl
a. Outdated red blood cells are concentrated, and stroma is removed. jl jl jl jl jl jl jl jl jl
b. Outdated red blood cells are diluted with saline, and stroma is removed. jl jl jl jl jl jl jl jl jl jl jl
c. Outdated red blood cells are lysed, and stroma is removed. jl jl jl jl jl jl jl jl jl
d. None of the above jl jl jl
j l 28. What is the normal life span of an RBC?
j l jl jl jl jl jl jl jl jl
a. 100 days jl c. 120 hours jl
b. 120 days jl d. 2 days j l jl
jl 29. Regarding loss of RBC membrane deformability, all of the following are true except:
j l jl jl jl jl jl jl jl jl jl jl jl jl
a. Increase in ATP level. jl jl jl
b. Decrease in ATP level. jl jl jl
Thisjlstudyjlsourcejlwasjldownloadedjlbyjl100000826342158jlfromjlCourseHero.comjlonjl09-15-2021jl17:54:15jlGMTjl-05:00
https://www.coursehero.com/file/25537437/ch01rtf/
