Microbiology Lab Practical 1 Study guide Questions and Answers 100% Pass

Microbiology Lab Practical 1 Study guide Questions and Answers 100% Pass Illuminator - Filter for shorter λ Condenser microscope - Focuses light into specimen Diaphragm (iris) - Controls amount of light to specimen Objective lenses at nosepiece - Magnifies image 10, 40, or 100 times Ocular lens - magnifies image 10X Course adjustment - focuses specimen in a broad way Fine adjustment - fine focuses specimen Stage - a platform used to hold the slide containing the specimen Arm - serves as a handle for carrying microscope Mechanical stage adjuster - moves stage forward and back Stage clip - Holds microscope slide in position Magnification - increase in apparent size Resolution - clarity Oil has the __________ refractive index as glass - same 2 | P a g e Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE This is the part of the microscope that should not be touched under high power. - the course adjustment knob How does the light intensity change when a student goes from a low power objective to a high power objective? - The field of vision narrowed Which lens do you use oil with? - 100X lens How does immersion oil work to enhance resolution? - Same refractive index as glass; prevents loss of light to air What is the maximum resolution of your microscope (in micrometers) or how far apart do two organisms have to be from one another in order to see them as 2 different organisms under the scope? - .2 um with 100X What is the total magnification of your scope when you use each lens? - ocular lens magnification times the magnification of the objective lens 10X x 40X, 100X, 10X What do you have to do to your microscope before you put it away? - lower the stage If your cells look washed out under the scope what can you do to fix the lighting - Adjust the lighting by using diaphragm or condenser Binocular versus monocular - Binocular has two eyepieces. Monocular has one eyepiece. Inorder to increase the resolution under the 100X oil objective, you must open this part of the microscope. - diaphragm path of light through a microscope and tell what happens after the light passes through each part. - Eukaryote - have a nucleus, membrane bound organelles and are larger than prokaryotes Prokaryote - no nucleus, no membrane bound organelles and are small 3 | P a g e Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE If you went from the 40X objective to the 100X objective and the cell you were looking at disappeared, give several explanations explaining what could have happened and how you could fix the problem. - Field of vision narrowed—center specimen Not enough light—open diaphragm Slightly out of focus—use fine focus The lens is not clicked all the way in place Cellular morphology - cell shape 3 types of cell shapes CBS - coccus (spheres), bacillus (rods), and spirillum (spiral) Field of vision - area you can see, smaller at higher powers; must CENTER object or you will lose it at higher power Refractive index - the ratio of light in a vacuum to its velocity in a specified medium Parfocal - when you can switch from one power to the next and you would only need to slightly fine focus with fine-adjustment knob to get specimen into view. 2 ways in which mold colonies differ from bacterial colonies under the dissecting scope - Mold colonies can't really be counted because they grow irregularly and in odd shapes. Bacteria colonies can be counted. Mold colonies are big and fuzzy looking while bacteria colonies are small, colorful, smooth or rough looking. Mold colonies usually look grey. Difference between colonial morphology from cellular morphology - Colonial morphology describes characteristics of individual colonies of bacteria growing on agar in petri dis