QBM Exam 3 Questions And Answers With Complete Solutions 2024

QBM Exam 3 Questions And Answers With Complete Solutions 2024 In a phenol-chloroform extraction, DNA will be in the aqueous phase - answerWhich is true regarding DNA purification? (a) In a phenol-chloroform extraction, DNA will be in the aqueous phase (b) the most common way to purify plasmid DNA is a cesium chloride gradient (c) the best way to purify DNA is via anion exchange chromatography (d) DNA w/ an A260:A280 of 1.25 is pure (e) a miniprep will produce -1 mg of DNA PolyTs - answerIf you are trying to purify mRNA, you should use a column that contains Generally DNA runs, from fastest to slowest: supercoiled, linear, circular, nicked - answerWhich of the following is true? (a) DNA gel electrophoresis req. SDS (b) DNA has a built-in-mass-to-charge ratio, so separates on gel electrophoresis based on both size and charge (c) DNA is loaded into the gel in line w/ the direction of migration (the current) (d) DNA gel electrophoresis can be used as a purification technique using the Chomczynski and Sacchi protocol (e) Generally DNA runs, from fastest to slowest: supercoiled, linear, circular, nicked Only a small amo. of DNA is generally necessary for downstream experiments, whereas protein experiments usually need a lot of sample - answerWhy can agarose gel electrophoresis be used as a purification step, as opposed to protein gels? (a) while both DNA and proteins are boiled, denatured, heated, and placed in an electric field, protein is more resistant to the process (b) only a small amo. of DNA is generally necessary for downstream experiments, whereas protein experiments usually need a lot of sample (c) more DNA is loaded on a gel than proteins on protein gels, so more can be purified (d)DNA is easier to visualize and can therefore be more readily purified from a gel (e) DNA gels use EDTA to protect it from DNases, whereas proteins are susceptible to proteases Primases - answerAll of the following are directly used in recombinant DNA technology EXCEPT: (a) DNA polymeras