MIP 300 Exam 1 Study Guides question n answers graded A+

MIP 300 Exam 1 Study GuidesCompare and contrast prokaryotic cells and eukaryotic cells - correct answer Prokaryotic cells have no nucleus or membrane organelles. Eurkaryotic cells have a nucleus and membrane bound organelles (golgi apparatus, ER, mitochondria, and lysosomes) List 4 types of cellular microbes and give a common example of each of these types of microbes - correct answer Fungi (yeast), Protozoa (lamblia causes giardia), Algae, Bacteria (mycobacterium), and Archea (themophilius) List 2 types of acellular microbes and describe what each of these acellular microbes is made of. - correct answer viruses (protein shell and nucleic acids), prions (protein), and viroids (RNA) Explain what a "transitional organism" is and name a type of microbe that can be transitional. - correct answer Protozoan because some may lack one or more of thes membrane bound organelles Explain how all living things are classified. - correct answer 3 Domains: Bacteria, Archaea, and Eukarya Describe how a bacterial genus is further broken down into groups. - correct answer Genus--> species--> strains Relate resolving power of a microscope to microbe size - correct answer The higher the resolution, the smaller microbe size you can see Explain which microbes can be seen using a light microscope and which ones cannot and why - correct answer most bacteria and small organelles Put microbes in order based on their size from smallest to largest - correct answer virus--> bacteria--> human cells organelles inside a eukaryotic microbe - correct answer phase contrast microscopy bacteria sample to visualize arrangement, cell shape and size - correct answer simple stain viruses in infected cells - correct answer electron microscope live bacteria in a water sample - correct answer bright field yeast cells used in the beer brewing process - correct answer fluorescent bright field microscopy - correct answer Background appears light and bacteria is the color of the stain. Must heat fix and stain bacteria first Dark field miscroscopy - correct answer Background appears dark and organisms appear light. Light is refracted by organisms Phase contrast microscopy - correct answer converts slight differences in refractive indexes into easily detected variations in light intensity fluorescent microscopy - correct answer Uses UV light to excite fluorescent molecules electron microscope - correct answer resolution is better and uses a light beam, can see viruses simple stain v differential stain - correct answer simple stain, 1 stain is used to increase microbes visibility. Differential stain divides microbes into different categories depending on structural characteristics Example of a differential stain - correct answer gram stain, acid-fast stain Gram stain - correct answer separates bacteria by the type of cell wall they have (+ or -) Acid fast stain - correct answer identified bacteria that are neither gram + or - that have a thick, waxy layer around them endospore stain - correct answer identifies spore-producing bacteria Flagella stain - correct answer identifies bacteria that possess flagella, used for motility Mycolic acid - correct answer long fatty acids that are found in mycobacterium cocci - correct answer spherical Diplococci - correct answer divide and remain together, pairs streptococci - correct answer divide and remain together, long chains staphylococci - correct answer divided in random planes, grape like clusters tetrads - correct answer divided in 2 planes, 4 cell groups Sarcina - correct answer divided in 3 planes, 8 cell groups Bacillus - correct answer rods coccobacillus - correct answer short and wide diplobacillus - correct answer remain together, pairs streptobacillus - correct answer remain together, long chains