BOC Study Guide Histotechnology 2nd ed.- Fixation (HT) fully solved graded A+ 2023/2024

BOC Study Guide Histotechnology 2nd ed.- Fixation (HT)An example of an additive fixative is one that contains: - correct answers picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: - correct answers incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from: - correct answers delayed fixation When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: - correct answers superior nuclear detail A certain project requires a fixative that contains acetic acid yet stabilizes erythrocyte membranes. One fixative that could be used is: - correct answers Hollande solution A specimen of kidney must be shipped to another city for immunofluorescence studies. The specimen should be placed in: - correct answers Michel Solution When the microwave oven is used for fixation, the most critical factor is the: - correct answers control of temperature To adequately remove the calcium from a specimen containing areas of microcalcification, the tissue should be fixed in: - correct answers Hollande solution Which of the following fixatives contains copper acetate? - correct answers Hollande A specimen is submitted with the statement that it was fixed in formalin. Microscopic sections show marked lysis of erythrocytes. This indicates that the fixative most likely was: - correct answers acidified with acetic acid Fixatives are classified as additive because of the: - correct answers addition, or binding of the fixative to tissue proteins. Kidney biopsy tissue has been fixed in phosphate-buffered glutaraldehyde for 2 hours and then placed in phosphate buffer solution. If a portion of this tisssue is processed for light microscopy, sections would most likely show: - correct answers nonspecific PAS staining Uric acid crystals are preserved ONLY when tissue is fixed in: - correct answers absolute alcohol Improper preservation of tissue will result if there is: - correct answers a delay in fixation A good fixative will: - correct answers protect tissue against alteration during subsequent processing. The function of methanol in commercial formalin solutions is to: - correct answers retard the polymerization of formaldehyde In electron microscopy, Zamboni fluid, glutaraldehyde, and osmium tetroxide function as: - correct answers fixative solutions Tissue will remain unfixed if placed in: - correct answers sodium borate Bouin Solution is contraindicated for: - correct answers tissue to be stained by the Feulgen reaction Formalin pigment can be removed from tissue sections by treatment with 10%: - correct answers ammonium hydroxide in 70% alcohol Stock neutralized formalin is prepared in the laboratory by storing the solution over a layer of calcium carbonate. The solution withdrawn from this stock container will: - correct answers become acidic