(MB) ASCP Exam Questions & Answers 2023

What kind of bond do histones have with DNA? - Answer Ionic What amino acids compose histones? - Answer Lysine and Arginine Which lymphoma has t-cell rearrangements? - Answer Sezary Syndrome Acute Promyelocytic leukemia translocation? - Answer t(15,17) Leading strand polymerase: - Answer Delta DNA repair polymerase(s): - Answer Beta or Epsilon Mitochondrial polymerase: - Answer Gamma Molecular beacon design: - Answer (R-sequence-Q) Which method uses visible light? - Answer Pyrosequencing Lagging strand polymerase: - Answer Alpha In NGS what is coverage referring to - Answer # of times a base is read Which type of NGS is best used for small indels? - Answer paired-end Number of SNPs in human genome? - Answer 10^7 book answer 10^6 exam answer (closest answer available) FLT3 is involved in what disease? - Answer AML RNA storage condition for 6 months? - Answer -70C in Ethanol How long to keep obsolete document in lab according to CAP? - Answer 2 years HIV viral load difference? - Answer 0.5 log 10 Antibiotic resistance mechanism in MRSA? - Answer altered drug target PBP2a mecA What is the effect of Irinotecan and UGT1A1? - Answer Decrease drug metabolism What part of RNA increases half-life and stability? - Answer G cap or 5' cap Formamide decreases Tm by: - Answer altering H-bonds Common form of DNA is: - Answer B form, Right-Handed Order to add to a PCR reaction - Answer Master mix Patient sample Positive control Negative control Primer dimers are formed due to: - Answer 3' Complementarity Virus affecting patients after kidney transplant is - Answer BK Virus Warfarin is associated with? - Answer VKORC1 and CYP2C9 Which is most specific PCR? - Answer Nested PCR then Real Time PCR What is the proper GC content of primer? - Answer 50-60% Which sample type to expect degraded DNA from? - Answer FFPE Highest vancomycin resistance? - Answer vanA HCV genotype 1 vs 2 : Which has a low viral load and best sustained response to drugs? - Answer HCV Genotype 2 To avoid RNAse at what temp should you bake the glassware? - Answer 250*C for 4 hours Aldehyde dehydrogenase question - Answer Alcohol dependence Metabolizing alcohol Thermal devices are verified by which agency? - Answer NIST How long can blood be stored at 4degC for DNA analysis? - Answer 72 hours Before a test can be implemented what metrics are required? - Answer waived, moderate complexity , high complexity What gel do you use for 200-250 bp? - Answer 6% Polyacrylamide Gel A sample was spiked with a positive control and results were negative for positive control and the spiked sample. What happened? - Answer Sample had an inhibitor What is TP53 or P53? - Answer Oncogene bDNA characteristics: - Answer Uses alkaline phosphatase and amplifies the signal Sensitivity vs. Specificity - Answer Sensitivity - the ability of a test to correctly identify those with the disease (true positive) Specificity - the ability of the test to correctly identify those without the disease (true negative) Accuracy vs. Precision - Answer Accuracy - refers to the closeness of a measured value to a standard or known value Precision - refers to the closeness of two or more measurements to each other PPV - Answer Positive predictive value (PPV): the proportion of people with positive test results that actually have the disease (i.e. are correctly diagnosed with the disease) Stop Codon - Answer UAA, UAG, UGA Start Codon - Answer AUG (methionine) Sensitivity - Answer ability of a test to correctly identify those with the disease (true positive rate) Specificity - Answer ability of a test to correctly identify those without the disease (true negative rate) Carbamazepine (Tegretol) - Answer Epilepsy HLA-B 1502