Samenvatting tabel moleculair biologische technieken in find it, lose it & move it experimenten

MOLECULAIR BIOLOGISCHE TECHNIEKEN IN FIND IT, LOSE IT & MOVE IT EXPERIMENTEN


TYPE DNA RNA PROTEIN

FIND IT intact cell / tissue intact cell / tissue intact cell / tissue
- spatial genomics - spatial transcriptomics - immunocytochemistry (ICC)
- (fluorescent) in situ hybridisation

cell / tissue lysate cell / tissue lysate cell / tissue lysate
- short-read or long-read DNA sequencing - short-read or long-read RNA sequencing (bulk) - mass-spectrometry
- single-cell DNA sequencing - RT-(q)PCR - western blot
- epigenetic sequencing (methylation, ATACseq, HiC) - northern blot (old)
- southern blot - single-cell RNA sequencing

LOSE IT - homologous recombination in embryonic stem cells - morpholinos - nanobodies
- CRISPR genome editing (non homologous end - RNAi: dsRNA, siRNA, shRNA, morpholino - receptor antagonists
joining, homology directed repair, base editing) - antibodies

MOVE IT - transgenic overexpression - mRNA injection or electroporation - implantation of beads soaked in a
- meganucleases (Sci-I) - mRNA transfection or transduction in cells secreted factor
- transposases (sleeping beauty, Tol) - protein injection or electroporation
- random integration in oocytes - agonists of pathways
- transient transfection - protein transfection in cells
- knock-in using homologous recombination in
embryonic stem cells