Complete Test Bank Molecular Biology of
the Cell 6th Edition Questions &
Answers with rationales (Chapter 1-24)
One method used to make transgenic mice would be to - ANSWER inject DNA
containing the gene into the pronucleus of a fertilized egg.
Expression of a human protein in a bacterial cell is best done with human - ANSWER
cDNA in a bacterial expression vector.
The nematode worm Caenorhabditis elegans (C. elegans) is a good model system
because it allowed researchers to - ANSWER follow the developmental lineage of all
the cells found in an adult from a single fertilized egg cell microscopically
To identify, in a sample, the protein against which you have an antibody, the best
technique to use would be _______ blotting. - ANSWER Western
The technique by which fragments of DNA are separated by gel electrophoresis,
blotted on nitrocellulose paper, labeled with a complementary radioactive probe, and
detected by autoradiography is called _______ blotting. - ANSWER Southern
Which of the following is included in a plasmid vector to facilitate successful cloning? -
ANSWER All of the Above (Origin of replication, gene conferring resistance to antibiotic,
and restriction endonuclease sites)
The method by which cell components are separated by centrifugation at progressively
higher speeds is called _______ centrifugation. - ANSWER Differential
Green fluorescent protein is commonly used to - ANSWER visualize the localization of
proteins in living cells.
DNA can be introduced into cells by - ANSWER All of the Above (microinjection into the
nucleus of a cell, fusion of DNA-containing liposomes with cells, and electroporation of
DNA into cells.)
Which of the following correctly describes a cDNA molecule? - ANSWER A DNA copy
of an RNA sequence synthesized by the enzyme reverse transcriptase
Which of the following is NOT an advantage of next generation sequencing technology
such as the Solexa Illumina or the 454 pyrosequencing system as compared to Sanger
dideoxy sequencing? - ANSWER Next generation sequencing produces longer reads
from each reaction (over 1000 base pairs) than one gets from a Sanger dideoxy
sequencing reaction
, Restriction endonucleases are enzymes that - ANSWER cleave DNA only at specific
sequences.
CRISPR-Cas9 is being used by scientists - ANSWER to knock out or alter genes in
chromosome of cells
Which of the following is a method one could use to isolate and purify the mitochondria
of cells away from other cell components? - ANSWER Density-gradient centrifugation
Which of the following techniques can be used to identify proteins that interact with one
another? - ANSWER yeast two-hybrid system
The _______ technique would be used to compare expression of 10,000 genes in two
different conditions (e.g., normal cells and cancer cells). - ANSWER DNA Microarray
Which of the following is a method that could be used to isolate just white blood cells
with CD4 proteins on their surface from all the other type of cells in a sample of blood? -
ANSWER Flow cytometry and FACS analysis
Which of the following correctly outlines the process of RNA interference beginning with
a double-stranded RNA molecule? - ANSWER Cleavage by Dicer; association with
RISC; pairing with target mRNA; mRNA cleavage
You are conducting a microarray experiment and have labeled the cDNAs generated
from cell line A1 with a red dye and the cDNA generated from cell line B2 with the green
dye. For gene spot 45, you observe that the color was green, what does that mean?
(modified sp 2018) - ANSWER The green fluorescence suggests reduced activity or
downregulation of this gene in cell line B2.
Active transport differs from facilitated diffusion in that - ANSWER active transport
involves the transport of molecules up or against their concentration gradient.
A feature common to almost all transmembrane proteins is - ANSWER a region of
about at least 19 hydrophobic amino acids.
Cholesterol affects the fluidity of membranes into which it inserts by - ANSWER
decreasing membrane fluidity at high temperatures and increasing membrane fluidity at
low temperatures.
Coupled transport of glucose and Na+ into the intestinal epithelial cell is an example of
- ANSWER symport
Facilitated diffusion differs from passive diffusion in that facilitated diffusion is -
ANSWER mediated by a protein carrier or channel.
the Cell 6th Edition Questions &
Answers with rationales (Chapter 1-24)
One method used to make transgenic mice would be to - ANSWER inject DNA
containing the gene into the pronucleus of a fertilized egg.
Expression of a human protein in a bacterial cell is best done with human - ANSWER
cDNA in a bacterial expression vector.
The nematode worm Caenorhabditis elegans (C. elegans) is a good model system
because it allowed researchers to - ANSWER follow the developmental lineage of all
the cells found in an adult from a single fertilized egg cell microscopically
To identify, in a sample, the protein against which you have an antibody, the best
technique to use would be _______ blotting. - ANSWER Western
The technique by which fragments of DNA are separated by gel electrophoresis,
blotted on nitrocellulose paper, labeled with a complementary radioactive probe, and
detected by autoradiography is called _______ blotting. - ANSWER Southern
Which of the following is included in a plasmid vector to facilitate successful cloning? -
ANSWER All of the Above (Origin of replication, gene conferring resistance to antibiotic,
and restriction endonuclease sites)
The method by which cell components are separated by centrifugation at progressively
higher speeds is called _______ centrifugation. - ANSWER Differential
Green fluorescent protein is commonly used to - ANSWER visualize the localization of
proteins in living cells.
DNA can be introduced into cells by - ANSWER All of the Above (microinjection into the
nucleus of a cell, fusion of DNA-containing liposomes with cells, and electroporation of
DNA into cells.)
Which of the following correctly describes a cDNA molecule? - ANSWER A DNA copy
of an RNA sequence synthesized by the enzyme reverse transcriptase
Which of the following is NOT an advantage of next generation sequencing technology
such as the Solexa Illumina or the 454 pyrosequencing system as compared to Sanger
dideoxy sequencing? - ANSWER Next generation sequencing produces longer reads
from each reaction (over 1000 base pairs) than one gets from a Sanger dideoxy
sequencing reaction
, Restriction endonucleases are enzymes that - ANSWER cleave DNA only at specific
sequences.
CRISPR-Cas9 is being used by scientists - ANSWER to knock out or alter genes in
chromosome of cells
Which of the following is a method one could use to isolate and purify the mitochondria
of cells away from other cell components? - ANSWER Density-gradient centrifugation
Which of the following techniques can be used to identify proteins that interact with one
another? - ANSWER yeast two-hybrid system
The _______ technique would be used to compare expression of 10,000 genes in two
different conditions (e.g., normal cells and cancer cells). - ANSWER DNA Microarray
Which of the following is a method that could be used to isolate just white blood cells
with CD4 proteins on their surface from all the other type of cells in a sample of blood? -
ANSWER Flow cytometry and FACS analysis
Which of the following correctly outlines the process of RNA interference beginning with
a double-stranded RNA molecule? - ANSWER Cleavage by Dicer; association with
RISC; pairing with target mRNA; mRNA cleavage
You are conducting a microarray experiment and have labeled the cDNAs generated
from cell line A1 with a red dye and the cDNA generated from cell line B2 with the green
dye. For gene spot 45, you observe that the color was green, what does that mean?
(modified sp 2018) - ANSWER The green fluorescence suggests reduced activity or
downregulation of this gene in cell line B2.
Active transport differs from facilitated diffusion in that - ANSWER active transport
involves the transport of molecules up or against their concentration gradient.
A feature common to almost all transmembrane proteins is - ANSWER a region of
about at least 19 hydrophobic amino acids.
Cholesterol affects the fluidity of membranes into which it inserts by - ANSWER
decreasing membrane fluidity at high temperatures and increasing membrane fluidity at
low temperatures.
Coupled transport of glucose and Na+ into the intestinal epithelial cell is an example of
- ANSWER symport
Facilitated diffusion differs from passive diffusion in that facilitated diffusion is -
ANSWER mediated by a protein carrier or channel.
