9/28/25, 3:14 PM
WGU Biochemistry Final Review EXAM 2025-2026
\COMPLETE QUESTIONS AND ACCURATE DETAILED
ANSWERS \VERIFIED 100% ALREADY GRADEDC A+
\LATEST UPDATE 2025-2026
Terms in this set (225)
True
Feedback
For PCR, in each cycle, the two strands of the duplex DNA
are separated by heating, then the reaction mixture is
cooled to allow the primers to anneal (or pair) to their
complementary segments on the DNA.
Next, the DNA polymerase directs the synthesis of the
complementary strands. The use of a heat-stable DNA
polymerase eliminates the need to add fresh enzyme
A thermocycler is a after each round of heating (heat inactivates most
machine used for PCR that enzymes). Hence, in the presence of sufficient quantities
of primers and dNTPs, PCR is carried out
varies the temperature of a
simply by cycling through the different temperatures for
sample. strand separation, primer annealing, and DNA synthesis.
The thermocycler is the machine that is used to vary
the temperature of the samples.
True False
The polymerase chain False
reaction is a tool used to
study protein structure. Feedback
PCR is a tool used to amplify a specific segment of
True DNA.
False
Red
/ 1/154
,9/28/25, 11:38 PM WGU Biochemistry Final Review
What color is the primer in Feedback
the following diagram? Recall from the section on DNA replication that DNA
polymerase needs a primer to begin DNA synthesis.
Red This requirement means the primers will direct the
Purple DNA polymerase to only synthesize complementary
Blue strands of the target DNA. (Note: In DNA replication,
the primers are RNA primers, while PCR generally
uses DNA primers because they are more stable.)
dNTPs are DNA True
nucleotides used in PCR.
Feedback
True dNTPs stands for deoxynucleotide triphosphates,
False which are the nucleotides used in DNA synthesis.
DNA polymerase is True
used in DNA
replication and in PCR. Feedback
True False Both PCR and DNA replication require DNA polymerase to
make new copies of DNA.
2/154
,9/28/25, 11:38 PM WGU Biochemistry Final Review
Which of the following Epigenetic changes
changes can NOT be
detected using PCR? Feedback
Epigenetic changes do not affect the sequence of the
Differences in DNA DNA. PCR is used to look at the DNA sequence.
sequence
Epigenetic changes
Deletions
Insertions
Which of the following RNA polymerase
components is NOT used in
PCR? Feedback
PCR makes a DNA copy, so DNA polymerase is used.
DNA template
DNA nucleotides RNA
polymerase Primers
3/154
, 9/28/25, 11:38 PM WGU Biochemistry Final Review
DNA polymerase can 5' to 3'
synthesize new DNA
strands in which direction? Feedback
DNA needs a free 3' end to bind to and initiate
3' to 5' synthesis of a DNA. It synthesizes in a 5' to 3' direction.
5' to 3'
any direction
N-terminus to C-terminus
Assuming there is one copy 32
of the target DNA sequence
before PCR, how many Feedback
Each cycles double the numbers of DNA copy. Round 1: 1->2.
copies of DNA are there
Round 2: 2 ->4. Round 3: 4 -> 8. Round 4: 8 ->16.
after 5 PCR Round 5: 16 ->32.
cycles?
256
6
65,536
16
32
4/154
WGU Biochemistry Final Review EXAM 2025-2026
\COMPLETE QUESTIONS AND ACCURATE DETAILED
ANSWERS \VERIFIED 100% ALREADY GRADEDC A+
\LATEST UPDATE 2025-2026
Terms in this set (225)
True
Feedback
For PCR, in each cycle, the two strands of the duplex DNA
are separated by heating, then the reaction mixture is
cooled to allow the primers to anneal (or pair) to their
complementary segments on the DNA.
Next, the DNA polymerase directs the synthesis of the
complementary strands. The use of a heat-stable DNA
polymerase eliminates the need to add fresh enzyme
A thermocycler is a after each round of heating (heat inactivates most
machine used for PCR that enzymes). Hence, in the presence of sufficient quantities
of primers and dNTPs, PCR is carried out
varies the temperature of a
simply by cycling through the different temperatures for
sample. strand separation, primer annealing, and DNA synthesis.
The thermocycler is the machine that is used to vary
the temperature of the samples.
True False
The polymerase chain False
reaction is a tool used to
study protein structure. Feedback
PCR is a tool used to amplify a specific segment of
True DNA.
False
Red
/ 1/154
,9/28/25, 11:38 PM WGU Biochemistry Final Review
What color is the primer in Feedback
the following diagram? Recall from the section on DNA replication that DNA
polymerase needs a primer to begin DNA synthesis.
Red This requirement means the primers will direct the
Purple DNA polymerase to only synthesize complementary
Blue strands of the target DNA. (Note: In DNA replication,
the primers are RNA primers, while PCR generally
uses DNA primers because they are more stable.)
dNTPs are DNA True
nucleotides used in PCR.
Feedback
True dNTPs stands for deoxynucleotide triphosphates,
False which are the nucleotides used in DNA synthesis.
DNA polymerase is True
used in DNA
replication and in PCR. Feedback
True False Both PCR and DNA replication require DNA polymerase to
make new copies of DNA.
2/154
,9/28/25, 11:38 PM WGU Biochemistry Final Review
Which of the following Epigenetic changes
changes can NOT be
detected using PCR? Feedback
Epigenetic changes do not affect the sequence of the
Differences in DNA DNA. PCR is used to look at the DNA sequence.
sequence
Epigenetic changes
Deletions
Insertions
Which of the following RNA polymerase
components is NOT used in
PCR? Feedback
PCR makes a DNA copy, so DNA polymerase is used.
DNA template
DNA nucleotides RNA
polymerase Primers
3/154
, 9/28/25, 11:38 PM WGU Biochemistry Final Review
DNA polymerase can 5' to 3'
synthesize new DNA
strands in which direction? Feedback
DNA needs a free 3' end to bind to and initiate
3' to 5' synthesis of a DNA. It synthesizes in a 5' to 3' direction.
5' to 3'
any direction
N-terminus to C-terminus
Assuming there is one copy 32
of the target DNA sequence
before PCR, how many Feedback
Each cycles double the numbers of DNA copy. Round 1: 1->2.
copies of DNA are there
Round 2: 2 ->4. Round 3: 4 -> 8. Round 4: 8 ->16.
after 5 PCR Round 5: 16 ->32.
cycles?
256
6
65,536
16
32
4/154
