1
CELLULAR AND MOLECULAR BIOLOGY FINAL EXAM
NEWEST ACTUAL EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS WITH RATIONALES (VERIFIED
ANSWERS) |ALREADY GRADED A+
On a micropipette that reads 20-200 on the plunger: - (ANSWER)The accurate
range of this micropipette is between 20 and 200 microliters
To determine the number of viable cells in an overnight culture of E.coli, the
following must be done: - (ANSWER)Dilution and spread plating
You have a 10 mL overnight culture of E.coli (original culture). You remove 0.1mL
of the E.coli culture and place it into a tube containing 9.9 mL of saline. You made
a: - (ANSWER)1:100 dilution of the original culture
You have a 1 mL overnight culture of E.coli (original culture). You remove 0.1 mL
of the E.coli culture and place it into a tube containing 9.9 mL of saline. You made
a: - (ANSWER)1:100 dilution of the original culture
You have a 100 mL overnight culture of E.coli (original culture). You remove 5 mL
of the E.coli culture and place it into a tube containing 45 mL of saline. You made
a: - (ANSWER)1:10 dilution of the original culture
Using a micropipette to transfer a small volume of solution between tubes: -
(ANSWER)is only accurate if used properly
, 2
A micropipette that reads 2-20 on the plunger... - (ANSWER)...can accurately
deliver between 2-20 microliter volumes
You are asked to transfer 0.010 mL of solution A into a fresh tube: -
(ANSWER)you should use a 2-20 micropipette
If you place 0.1 mL of solution A into 9.9 mL of water, how much has solution A
been diluted? - (ANSWER)1:100
You are asked to put 116 microliters of solution A into solution B: - (ANSWER)all
of the answers are theoretically correct, but some of the options may be better
than others
You have made a 1:10000 dilution of E.coli (original solution). After spread plating
0.1mL of the diluted solution, you obtained 100 colonies on a TSA plate after
incubation for 24 hours. How many viable cells are present in 0.1 mL of the
original solution? - (ANSWER)1000000
You have made a 1:100000 dilution of E.coli (original solution). After spread
plating 0.1 mL of the diluted solution, you obtained 100 colonies on a TSA plate
after incubation for 24 hours. How many viable cells are present in 0.1 mL of the
original solution? - (ANSWER)10000000
You have made a 1:100000 dilution of E.coli (original solution). After spread
plating 0.1 mL of the diluted solution, you obtained 100 colonies on a TSA plate
CELLULAR AND MOLECULAR BIOLOGY FINAL EXAM
NEWEST ACTUAL EXAM QUESTIONS AND CORRECT
DETAILED ANSWERS WITH RATIONALES (VERIFIED
ANSWERS) |ALREADY GRADED A+
On a micropipette that reads 20-200 on the plunger: - (ANSWER)The accurate
range of this micropipette is between 20 and 200 microliters
To determine the number of viable cells in an overnight culture of E.coli, the
following must be done: - (ANSWER)Dilution and spread plating
You have a 10 mL overnight culture of E.coli (original culture). You remove 0.1mL
of the E.coli culture and place it into a tube containing 9.9 mL of saline. You made
a: - (ANSWER)1:100 dilution of the original culture
You have a 1 mL overnight culture of E.coli (original culture). You remove 0.1 mL
of the E.coli culture and place it into a tube containing 9.9 mL of saline. You made
a: - (ANSWER)1:100 dilution of the original culture
You have a 100 mL overnight culture of E.coli (original culture). You remove 5 mL
of the E.coli culture and place it into a tube containing 45 mL of saline. You made
a: - (ANSWER)1:10 dilution of the original culture
Using a micropipette to transfer a small volume of solution between tubes: -
(ANSWER)is only accurate if used properly
, 2
A micropipette that reads 2-20 on the plunger... - (ANSWER)...can accurately
deliver between 2-20 microliter volumes
You are asked to transfer 0.010 mL of solution A into a fresh tube: -
(ANSWER)you should use a 2-20 micropipette
If you place 0.1 mL of solution A into 9.9 mL of water, how much has solution A
been diluted? - (ANSWER)1:100
You are asked to put 116 microliters of solution A into solution B: - (ANSWER)all
of the answers are theoretically correct, but some of the options may be better
than others
You have made a 1:10000 dilution of E.coli (original solution). After spread plating
0.1mL of the diluted solution, you obtained 100 colonies on a TSA plate after
incubation for 24 hours. How many viable cells are present in 0.1 mL of the
original solution? - (ANSWER)1000000
You have made a 1:100000 dilution of E.coli (original solution). After spread
plating 0.1 mL of the diluted solution, you obtained 100 colonies on a TSA plate
after incubation for 24 hours. How many viable cells are present in 0.1 mL of the
original solution? - (ANSWER)10000000
You have made a 1:100000 dilution of E.coli (original solution). After spread
plating 0.1 mL of the diluted solution, you obtained 100 colonies on a TSA plate
