UNE (University Of New England) Medical Biochemistry
Midterm Exam | Featuring Topics such as Metabolic
Pathways, Enzymatic Function, Bioenergetics,
Carbohydrate and Lipid Metabolism, Protein Structure,
and Clinical Correlations
A teenage female presenting with lack of menstruation (amenorrhea) is found to
have dysfunctional steroid receptor characteristic of androgen insensitivity
syndrome. Which of the following statements accurately describes steroid
hormones? ---------CORRECT ANSWER-----------------The enhance transcription
when bound to receptors.
A deficiency in thiamine can reduce the activity of the pyruvate dehydrogenase
complex. Under these conditions, you would expect to have a higher than normal
blood concentration of which of the following metabolites? ---------CORRECT
ANSWER-----------------Pyruvate
Which of the following outlines the order of prosthetic groups as they are used
for catalysis within the pyruvate dehydrogenase complex (PDC)? ---------CORRECT
ANSWER-----------------Thiamine diphosphate-> lipoamide -> FAD
After a meal, fructose 2,6-bisphosphate levels are elevated. This compound will
allosterically iactivate which of the following glycolytic enzymes? ---------CORRECT
ANSWER-----------------Phosphofructokinase -1 (PFK1)
,In order for glucose to be retained within the cell, which of the following
modifications must occur? ---------CORRECT ANSWER-----------------Phosphorylation
Which of the following reactions is commonly used to replenish intermediates of
the TCA cycle? ---------CORRECT ANSWER-----------------Glutamate to alpha
ketoglutarate
Which of the following enzymes is correctly paired with its activator? ---------
CORRECT ANSWER-----------------Pyruvate dehydrogenase complex; pyruvate
Which of the following intermediates of the TCA cycle is required in the cytosol
for fatty acid synthesis? ---------CORRECT ANSWER-----------------Citrate
Pyruvate dehydrogenase kinase catalyzes a reaction that results in which of the
following? ---------CORRECT ANSWER-----------------The phosphorylation and
inactivation of E1 subunit of PDC
A 1-month old boy is brought to the emergency department in a coma after
sleeping through the night and failing to awaken in the morning. He is given
intravenous glucose and awakens. Serum levels of pyruvate, lactate, and alanine
, are elevated, whereas oxaloacetate and aspartate levels are reduced. Which of
the following is the most likely diagnosis in this patient? ---------CORRECT
ANSWER-----------------Pyruvate carboxylase deficiency
Which of the following enzymes is used by the gluconeogenic pathway but not
the glycolytic pathway? ---------CORRECT ANSWER-----------------
Phosphoenolpyruvate carboxykinase (PEPCK)
The skeletal muscle primarily provides substrate for gluconeogenesis through
which of the following processes? ---------CORRECT ANSWER-----------------Protein
catabolism
Which of the following would occur in the liver when glucose levels are high (think
insulin is elevated)? ---------CORRECT ANSWER-----------------Generation of glucose
1-phosphate to be used for glycogen synthesis
An individual is recently diagnosed with Type 2 diabetes. In this individual,
hyperglycemia may in part be due to inactivity of which of the following glucose
transporters on skeletal muscle? ---------CORRECT ANSWER-----------------GLUT4
Which of the following compounds allosterically activates pyruvate carboxylase? -
--------CORRECT ANSWER-----------------Acetyl-CoA
Midterm Exam | Featuring Topics such as Metabolic
Pathways, Enzymatic Function, Bioenergetics,
Carbohydrate and Lipid Metabolism, Protein Structure,
and Clinical Correlations
A teenage female presenting with lack of menstruation (amenorrhea) is found to
have dysfunctional steroid receptor characteristic of androgen insensitivity
syndrome. Which of the following statements accurately describes steroid
hormones? ---------CORRECT ANSWER-----------------The enhance transcription
when bound to receptors.
A deficiency in thiamine can reduce the activity of the pyruvate dehydrogenase
complex. Under these conditions, you would expect to have a higher than normal
blood concentration of which of the following metabolites? ---------CORRECT
ANSWER-----------------Pyruvate
Which of the following outlines the order of prosthetic groups as they are used
for catalysis within the pyruvate dehydrogenase complex (PDC)? ---------CORRECT
ANSWER-----------------Thiamine diphosphate-> lipoamide -> FAD
After a meal, fructose 2,6-bisphosphate levels are elevated. This compound will
allosterically iactivate which of the following glycolytic enzymes? ---------CORRECT
ANSWER-----------------Phosphofructokinase -1 (PFK1)
,In order for glucose to be retained within the cell, which of the following
modifications must occur? ---------CORRECT ANSWER-----------------Phosphorylation
Which of the following reactions is commonly used to replenish intermediates of
the TCA cycle? ---------CORRECT ANSWER-----------------Glutamate to alpha
ketoglutarate
Which of the following enzymes is correctly paired with its activator? ---------
CORRECT ANSWER-----------------Pyruvate dehydrogenase complex; pyruvate
Which of the following intermediates of the TCA cycle is required in the cytosol
for fatty acid synthesis? ---------CORRECT ANSWER-----------------Citrate
Pyruvate dehydrogenase kinase catalyzes a reaction that results in which of the
following? ---------CORRECT ANSWER-----------------The phosphorylation and
inactivation of E1 subunit of PDC
A 1-month old boy is brought to the emergency department in a coma after
sleeping through the night and failing to awaken in the morning. He is given
intravenous glucose and awakens. Serum levels of pyruvate, lactate, and alanine
, are elevated, whereas oxaloacetate and aspartate levels are reduced. Which of
the following is the most likely diagnosis in this patient? ---------CORRECT
ANSWER-----------------Pyruvate carboxylase deficiency
Which of the following enzymes is used by the gluconeogenic pathway but not
the glycolytic pathway? ---------CORRECT ANSWER-----------------
Phosphoenolpyruvate carboxykinase (PEPCK)
The skeletal muscle primarily provides substrate for gluconeogenesis through
which of the following processes? ---------CORRECT ANSWER-----------------Protein
catabolism
Which of the following would occur in the liver when glucose levels are high (think
insulin is elevated)? ---------CORRECT ANSWER-----------------Generation of glucose
1-phosphate to be used for glycogen synthesis
An individual is recently diagnosed with Type 2 diabetes. In this individual,
hyperglycemia may in part be due to inactivity of which of the following glucose
transporters on skeletal muscle? ---------CORRECT ANSWER-----------------GLUT4
Which of the following compounds allosterically activates pyruvate carboxylase? -
--------CORRECT ANSWER-----------------Acetyl-CoA
