Histotechnology Exam 1 Questions
(chapter 1) Questions and Answers
(Latest Update 2025) 100% Verified
Answers
What method is used to stabilize proteins making it resistant
to further damage? - Correct Answer ✅Fixation
Name two methods of fixation? - Correct Answer
✅Chemical reaction (formaldehyde) and physical (heat or
dessication)
What are two causes of Post mortem putrefaction if the tissue
is not fixed? ***** - Correct Answer ✅bacteria or mold
What factor causes autolysis if tissue is not fixed? **** -
Correct Answer ✅enzyme attack
What are four functions of fixatives? - Correct Answer ✅1.
kills tissue to prevent putrefaction and autolysis
2. maintains proper relationship between intracellular and
extracellular substances
3. preserves refractive indexes
4. enhances stains
What three organs contain the highest levels of enzymes? -
Correct Answer ✅liver, kidney, and brain
, Histotechnology Exam 1 Questions
(chapter 1) Questions and Answers
(Latest Update 2025) 100% Verified
Answers
True or False. You can over fix tissue? - Correct Answer
✅True. Over fixation dries out the tissue and limits the
quality. Can attempt steaming the sections to remedy over
fixation.
If you receive a breast sample on a Friday night, what method
must be performed to prevent exceeding the allowed formalin
fixation time to perform the Her2 test? - Correct Answer
✅Must place the tissue in 70% alcohol for one day and then
place in formalin the following day.
How quickly does formalin penetrate tissue? - Correct
Answer ✅1 mm per hour
True or False. When fixing a liver specimen, the fixation time
should be doubled? - Correct Answer ✅True. The enzymes
found in the cytoplasm need to be fixed well.
What is the name of a fixative that is combined with the
protein molecule? Give an example. - Correct Answer
✅additive fixative
Ex. formaldehyde
(chapter 1) Questions and Answers
(Latest Update 2025) 100% Verified
Answers
What method is used to stabilize proteins making it resistant
to further damage? - Correct Answer ✅Fixation
Name two methods of fixation? - Correct Answer
✅Chemical reaction (formaldehyde) and physical (heat or
dessication)
What are two causes of Post mortem putrefaction if the tissue
is not fixed? ***** - Correct Answer ✅bacteria or mold
What factor causes autolysis if tissue is not fixed? **** -
Correct Answer ✅enzyme attack
What are four functions of fixatives? - Correct Answer ✅1.
kills tissue to prevent putrefaction and autolysis
2. maintains proper relationship between intracellular and
extracellular substances
3. preserves refractive indexes
4. enhances stains
What three organs contain the highest levels of enzymes? -
Correct Answer ✅liver, kidney, and brain
, Histotechnology Exam 1 Questions
(chapter 1) Questions and Answers
(Latest Update 2025) 100% Verified
Answers
True or False. You can over fix tissue? - Correct Answer
✅True. Over fixation dries out the tissue and limits the
quality. Can attempt steaming the sections to remedy over
fixation.
If you receive a breast sample on a Friday night, what method
must be performed to prevent exceeding the allowed formalin
fixation time to perform the Her2 test? - Correct Answer
✅Must place the tissue in 70% alcohol for one day and then
place in formalin the following day.
How quickly does formalin penetrate tissue? - Correct
Answer ✅1 mm per hour
True or False. When fixing a liver specimen, the fixation time
should be doubled? - Correct Answer ✅True. The enzymes
found in the cytoplasm need to be fixed well.
What is the name of a fixative that is combined with the
protein molecule? Give an example. - Correct Answer
✅additive fixative
Ex. formaldehyde
