Portage Microbiology Exam #5
Study online at https://quizlet.com/_9v3sel
1. Define the concept of universal precautions (short Universal precau-
answer) tions means any
and all samples,
whether known or
unknown, are to be
treated as poten-
tially hazardous (or
pathogenic) materi-
als
2. List at lease 3 observations a researcher would be Need to note:
sure to note while assessing and unknown microbial 1. Size and shape
sample (short answer) 2. Any observable
motility
3. Gram status
(positive or nega-
tive)
3. While observing an unknown sample of limited You must deter-
amounts, a researcher must determine the following mine motility before
observations: 1) the presence of any motility and 2) determining Gram
its Gram status using the same sample- the liquid status. Motility re-
sample cannot be divided. Which would you deter- quires a wet mount,
mine first and why? (short answer) while gram stain-
ing requires heat
fixing the sample.
If one were to be-
gin with the gram
stain, the heat fixa-
tion process would
kill the organism,
making any ob-
servations regard-
ing motility impos-
sible. The correct
approach would be
to place the liq-
uid culture on a
glass slide and de-
, Portage Microbiology Exam #5
Study online at https://quizlet.com/_9v3sel
termine its motili-
ty status. Next, the
same liquid culture
can be heat fixed
and gram stained.
4. A facultative anaerobe is a microorganism capable of A facultative anaer-
growth under what conditions? (short answer) obe is capable
of growth under
aerobic (with oxy-
gen) and anaero-
bic (without oxy-
gen) conditions
5. True or False: The Lancefield groups are used to sub- False. The Lance-
divide antigenic groups of alpha-hemolytic Strepto- field groups are
coccus used to subdi-
vide beta-hemolytic
strep
6. The distinctions for Lancefield subgroups lie in its: B. Carbohydrate
(select all that apply) composition of anti-
A. Catalase activity gens
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above
7. Rheumatic fever displays _____ hemolytic activity Beta: strep.
and occurs when _____ if left untreated Pharyngitis
8. True or False: Under most circumstances, staphylo- False. Staphylo-
coccus can be found in ~80% of human population coccus in only
where it remains non-symptomatic found (non-sympto-
matic) in ~30% of
the human popula-
tion
9. Define commensal bacteria (short answer) (know The bacterium nei-
term and definition first) ther harms nor ben-
efits from the host
Study online at https://quizlet.com/_9v3sel
1. Define the concept of universal precautions (short Universal precau-
answer) tions means any
and all samples,
whether known or
unknown, are to be
treated as poten-
tially hazardous (or
pathogenic) materi-
als
2. List at lease 3 observations a researcher would be Need to note:
sure to note while assessing and unknown microbial 1. Size and shape
sample (short answer) 2. Any observable
motility
3. Gram status
(positive or nega-
tive)
3. While observing an unknown sample of limited You must deter-
amounts, a researcher must determine the following mine motility before
observations: 1) the presence of any motility and 2) determining Gram
its Gram status using the same sample- the liquid status. Motility re-
sample cannot be divided. Which would you deter- quires a wet mount,
mine first and why? (short answer) while gram stain-
ing requires heat
fixing the sample.
If one were to be-
gin with the gram
stain, the heat fixa-
tion process would
kill the organism,
making any ob-
servations regard-
ing motility impos-
sible. The correct
approach would be
to place the liq-
uid culture on a
glass slide and de-
, Portage Microbiology Exam #5
Study online at https://quizlet.com/_9v3sel
termine its motili-
ty status. Next, the
same liquid culture
can be heat fixed
and gram stained.
4. A facultative anaerobe is a microorganism capable of A facultative anaer-
growth under what conditions? (short answer) obe is capable
of growth under
aerobic (with oxy-
gen) and anaero-
bic (without oxy-
gen) conditions
5. True or False: The Lancefield groups are used to sub- False. The Lance-
divide antigenic groups of alpha-hemolytic Strepto- field groups are
coccus used to subdi-
vide beta-hemolytic
strep
6. The distinctions for Lancefield subgroups lie in its: B. Carbohydrate
(select all that apply) composition of anti-
A. Catalase activity gens
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above
7. Rheumatic fever displays _____ hemolytic activity Beta: strep.
and occurs when _____ if left untreated Pharyngitis
8. True or False: Under most circumstances, staphylo- False. Staphylo-
coccus can be found in ~80% of human population coccus in only
where it remains non-symptomatic found (non-sympto-
matic) in ~30% of
the human popula-
tion
9. Define commensal bacteria (short answer) (know The bacterium nei-
term and definition first) ther harms nor ben-
efits from the host
