1|Page
ASCP CG CERTIFICATION EXAM 2025 WITH 500
REAL EXAM PRACTICE QUESTIONS AND CORRECT
ANSWERS/ ASCP CG CERTIFICATION TEST 2025/
ASCP CYTOGENETICS TEST
What is the best agent to use against mycoplasma? -
..ANSWER...✓✓ BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely
targets mycoplasma growth
For successful harvest, each new batch of hypotonic
solution should be evaluated by: - ..ANSWER...✓✓
comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ..ANSWER...✓✓
Proteolytic enzymes - break down tissues into single-cell
suspensions
When harvesting peripheral blood cultures, asceptic
technique no longer matters following incubation with: -
..ANSWER...✓✓ Colcemid - mitotic spindle poison that
halts cells at metaphase
,2|Page
Samples that arrive at the cytogenetics lab should first
be: - ..ANSWER...✓✓ Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells
into division? - ..ANSWER...✓✓ T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial.
When logging a culture failure, what is the MOST
important to document? - ..ANSWER...✓✓ Expiration
dates of reagents used
Which type of microscope is used when cleaning a CVS
(chorionic villus) sample? - ..ANSWER...✓✓ Dissection
Which cell type is most problematic if found in a CVS
(chorionic villus) sample? - ..ANSWER...✓✓ Decidua
(maternal tissue)
When looking at a slide under a phase contrast scope and
a lot of cytoplasm is still seen around the mets, what is
the most likely cause? - ..ANSWER...✓✓ innappropriate
slide drying time
,3|Page
What will help prevent microbial contamination and cross
contamination of cultures? - ..ANSWER...✓✓ Asceptic
technique
When there are not enough mets to complete the study of
a patient/case, what needs to be done next? -
..ANSWER...✓✓ prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and
mets are very spread out and sparse, what could help fix
the issue? - ..ANSWER...✓✓ Increading the drying time
by using warm slides
What is true of an inverted microscope? - ..ANSWER...✓✓
the objectives are beneath the stage
High-resolution banding is indicated for a possible
diagnosis of: - ..ANSWER...✓✓ DiGenorge syndrome --
microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
, 4|Page
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum
(upper lip indent), tubular nose, wide spread eyes, etc
When verifying if a specimen is appropriate for
cytogenetic analysis, what is the MOST important data to
accompany the specimen? - ..ANSWER...✓✓ Reason for
referral
Which type of specimen should be requested for a
cytogenetic analysis for turner syndrome? -
..ANSWER...✓✓ Heparinized peripheral blood stored at
room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a
malignancy only if: - ..ANSWER...✓✓ Dividing leukemic
cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects
of the incubator are important to monitor to assess the
source of the problem? - ..ANSWER...✓✓ Temperature,
ASCP CG CERTIFICATION EXAM 2025 WITH 500
REAL EXAM PRACTICE QUESTIONS AND CORRECT
ANSWERS/ ASCP CG CERTIFICATION TEST 2025/
ASCP CYTOGENETICS TEST
What is the best agent to use against mycoplasma? -
..ANSWER...✓✓ BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely
targets mycoplasma growth
For successful harvest, each new batch of hypotonic
solution should be evaluated by: - ..ANSWER...✓✓
comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ..ANSWER...✓✓
Proteolytic enzymes - break down tissues into single-cell
suspensions
When harvesting peripheral blood cultures, asceptic
technique no longer matters following incubation with: -
..ANSWER...✓✓ Colcemid - mitotic spindle poison that
halts cells at metaphase
,2|Page
Samples that arrive at the cytogenetics lab should first
be: - ..ANSWER...✓✓ Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells
into division? - ..ANSWER...✓✓ T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial.
When logging a culture failure, what is the MOST
important to document? - ..ANSWER...✓✓ Expiration
dates of reagents used
Which type of microscope is used when cleaning a CVS
(chorionic villus) sample? - ..ANSWER...✓✓ Dissection
Which cell type is most problematic if found in a CVS
(chorionic villus) sample? - ..ANSWER...✓✓ Decidua
(maternal tissue)
When looking at a slide under a phase contrast scope and
a lot of cytoplasm is still seen around the mets, what is
the most likely cause? - ..ANSWER...✓✓ innappropriate
slide drying time
,3|Page
What will help prevent microbial contamination and cross
contamination of cultures? - ..ANSWER...✓✓ Asceptic
technique
When there are not enough mets to complete the study of
a patient/case, what needs to be done next? -
..ANSWER...✓✓ prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and
mets are very spread out and sparse, what could help fix
the issue? - ..ANSWER...✓✓ Increading the drying time
by using warm slides
What is true of an inverted microscope? - ..ANSWER...✓✓
the objectives are beneath the stage
High-resolution banding is indicated for a possible
diagnosis of: - ..ANSWER...✓✓ DiGenorge syndrome --
microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
, 4|Page
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum
(upper lip indent), tubular nose, wide spread eyes, etc
When verifying if a specimen is appropriate for
cytogenetic analysis, what is the MOST important data to
accompany the specimen? - ..ANSWER...✓✓ Reason for
referral
Which type of specimen should be requested for a
cytogenetic analysis for turner syndrome? -
..ANSWER...✓✓ Heparinized peripheral blood stored at
room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a
malignancy only if: - ..ANSWER...✓✓ Dividing leukemic
cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects
of the incubator are important to monitor to assess the
source of the problem? - ..ANSWER...✓✓ Temperature,
