MCB 2050 Final Exam Review: Key Words
2 (Microsopy)
Brightfield microscopy - -Sample is illuminated by white light that is transmitted through
the sample onto the detector.
Image is usually captured by a video camera, and is more sensitive to low light intensities
-Deconvolution - -Removes background and out-of-focus light
Yields higher contrast and clarity
-Magnification - -Objective lens x ocular lens
Purpose of microscopy to generate magnified, high quality view of specimen
-Resolution (what 2 factors does power depend on) - -Wavelength (lambda) of
illumination light and Numerical Aperture (NA)
-How is resolution maximized - -Use shorter lambda of illuminating light, or increase NA
-Formaldehyde fixation - -Cross links amino groups on adjacent amino acids
-Embedding - -Embedded in plastic wax for support
-What is sectioning done with - -Microtome
-Molecules can be _______ with molecule specific dies to be identified - -Stained
-Microscopy artifacts - -An artificial structure or tissue alteration on a prepared
microscopic slide as a result of an irrelevant factor
-Microtome - -Instrument for cutting thin slices of biological specimens for microscopy
-Fluorescence microscopy - -Technique for visualizing fluorescent molecules in living or
fixed specimens
-Autofluorescence - -Relies on endogenous fluorescence in specimen
-Immunofluorescence - -Applied fluorescent dyes or dye-conjugated antibodies
-Auto fluorescent proteins - -Emit light upon excitation without additional reagents
2 (Microsopy)
Brightfield microscopy - -Sample is illuminated by white light that is transmitted through
the sample onto the detector.
Image is usually captured by a video camera, and is more sensitive to low light intensities
-Deconvolution - -Removes background and out-of-focus light
Yields higher contrast and clarity
-Magnification - -Objective lens x ocular lens
Purpose of microscopy to generate magnified, high quality view of specimen
-Resolution (what 2 factors does power depend on) - -Wavelength (lambda) of
illumination light and Numerical Aperture (NA)
-How is resolution maximized - -Use shorter lambda of illuminating light, or increase NA
-Formaldehyde fixation - -Cross links amino groups on adjacent amino acids
-Embedding - -Embedded in plastic wax for support
-What is sectioning done with - -Microtome
-Molecules can be _______ with molecule specific dies to be identified - -Stained
-Microscopy artifacts - -An artificial structure or tissue alteration on a prepared
microscopic slide as a result of an irrelevant factor
-Microtome - -Instrument for cutting thin slices of biological specimens for microscopy
-Fluorescence microscopy - -Technique for visualizing fluorescent molecules in living or
fixed specimens
-Autofluorescence - -Relies on endogenous fluorescence in specimen
-Immunofluorescence - -Applied fluorescent dyes or dye-conjugated antibodies
-Auto fluorescent proteins - -Emit light upon excitation without additional reagents
