BIMM 112 F questions with 100%
correct answers rated A+
Is the DNA in a nucleosome wrapped in a left- or right-handed superhelix around the core
histone octamer?
left
right
left and right in alteration - correct answer ✔✔ left
Sort the following chromatin particles by length of their DNA fragments they contain. None = 0.
Histone Octamer < Nucleosome Core Particle < Chromosome < Nucleosome
Chromosome < Histone Octamer < Nucleosome Core Particle < Nucleosome
Nucleosome < Chromosome < Histone Octamer < Nucleosome Core Particle
Nucleosome Core Particle < Histone Octamer <Nucleosome < Chromosome - correct answer
✔✔ Histone Octamer < Nucleosome Core Particle < Chromosome < Nucleosome
Which of the following particles contain the linker Histone H1?
Histone Octamer
Chromosome
Nucleosome
Core Particle - correct answer ✔✔ Chromosome
Nucleosome
How do you call the enzyme used to digest chromatin to discover the different types of
particles?
DNaseI
,H2A/H2B
MNase
PNK - correct answer ✔✔ MNase
What is the "Repeat length" in the context of chromatin?
The number of nucleosomes within the DNA of interest
The the distance for the average amount of DNA per nucleosome in chromatin
The DNA lenght and number of nucleosomes that exist in the DNA - correct answer ✔✔ The the
distance for the average amount of DNA per nucleosome in chromatin
What do you call the halfway point of the path of the DNA around the core histone octamer
flanked by the DNA entry and exit points of the nucleosome?
MNase
The dyad
H3K4me3
The histone octamer - correct answer ✔✔ The dyad
In the context of histone modifications,
___that add modifications to the histones,
___are enzymes that remove modifications from the histones,
___are proteins that recognize and bind to the specific modification. - correct answer ✔✔
writers, erasers, readers
Histone tail modifications play an important role in gene regulation. In general _____ is
activating, ____ can be activating or repressive, while ___ is more associated with genome
stability. - correct answer ✔✔ Acetylation, Methylation,Phosphorylation
, What is the protein that binds specifically to H3K9me3? (the acronym is OK)? - correct answer
✔✔ HP1
You do a ChIP experiment with antibodies against trimethylated H3K27 with a gene in the
repressed state. You find that the H3K27me3 ChIP signal disappears as the gene becomes
activated.
Please state True/False if the following statements are a possible reasons why you might see the
loss of the ChIP signal:
Loss of trimethylation (i.e., demethylation) of H3K27me3.
Loss of H3 protein.
Loss of access of the antibodies to the H3K27me3. - correct answer ✔✔ t/t/t
H3K4 methylation is essential for transcriptional regulation in fruit flies (Drosophila
melanogaster)? (T/F) - correct answer ✔✔ F
Does RNA interference reduce or eliminate the target transcript? - correct answer ✔✔ reduce
The cofactor used as the source of acetyl groups by histone acetyl transferases (KATs) is
____while the cofactor is used as the source of methyl groups by histone methyl
transferases____ (KMTs) is . - correct answer ✔✔ acetyl-CoA and S-adenosylmethionine (SAM)
The key reagent that allows you to detect where your protein associates with DNA in ChIP-seq is
called - correct answer ✔✔ antibody
Which statements do best describe Euchromatin or Heterochromatin? Please copy the word
behind the statement that best describes it.
is located at the periphery of nuclei.
is rich in repetitive DNA.
correct answers rated A+
Is the DNA in a nucleosome wrapped in a left- or right-handed superhelix around the core
histone octamer?
left
right
left and right in alteration - correct answer ✔✔ left
Sort the following chromatin particles by length of their DNA fragments they contain. None = 0.
Histone Octamer < Nucleosome Core Particle < Chromosome < Nucleosome
Chromosome < Histone Octamer < Nucleosome Core Particle < Nucleosome
Nucleosome < Chromosome < Histone Octamer < Nucleosome Core Particle
Nucleosome Core Particle < Histone Octamer <Nucleosome < Chromosome - correct answer
✔✔ Histone Octamer < Nucleosome Core Particle < Chromosome < Nucleosome
Which of the following particles contain the linker Histone H1?
Histone Octamer
Chromosome
Nucleosome
Core Particle - correct answer ✔✔ Chromosome
Nucleosome
How do you call the enzyme used to digest chromatin to discover the different types of
particles?
DNaseI
,H2A/H2B
MNase
PNK - correct answer ✔✔ MNase
What is the "Repeat length" in the context of chromatin?
The number of nucleosomes within the DNA of interest
The the distance for the average amount of DNA per nucleosome in chromatin
The DNA lenght and number of nucleosomes that exist in the DNA - correct answer ✔✔ The the
distance for the average amount of DNA per nucleosome in chromatin
What do you call the halfway point of the path of the DNA around the core histone octamer
flanked by the DNA entry and exit points of the nucleosome?
MNase
The dyad
H3K4me3
The histone octamer - correct answer ✔✔ The dyad
In the context of histone modifications,
___that add modifications to the histones,
___are enzymes that remove modifications from the histones,
___are proteins that recognize and bind to the specific modification. - correct answer ✔✔
writers, erasers, readers
Histone tail modifications play an important role in gene regulation. In general _____ is
activating, ____ can be activating or repressive, while ___ is more associated with genome
stability. - correct answer ✔✔ Acetylation, Methylation,Phosphorylation
, What is the protein that binds specifically to H3K9me3? (the acronym is OK)? - correct answer
✔✔ HP1
You do a ChIP experiment with antibodies against trimethylated H3K27 with a gene in the
repressed state. You find that the H3K27me3 ChIP signal disappears as the gene becomes
activated.
Please state True/False if the following statements are a possible reasons why you might see the
loss of the ChIP signal:
Loss of trimethylation (i.e., demethylation) of H3K27me3.
Loss of H3 protein.
Loss of access of the antibodies to the H3K27me3. - correct answer ✔✔ t/t/t
H3K4 methylation is essential for transcriptional regulation in fruit flies (Drosophila
melanogaster)? (T/F) - correct answer ✔✔ F
Does RNA interference reduce or eliminate the target transcript? - correct answer ✔✔ reduce
The cofactor used as the source of acetyl groups by histone acetyl transferases (KATs) is
____while the cofactor is used as the source of methyl groups by histone methyl
transferases____ (KMTs) is . - correct answer ✔✔ acetyl-CoA and S-adenosylmethionine (SAM)
The key reagent that allows you to detect where your protein associates with DNA in ChIP-seq is
called - correct answer ✔✔ antibody
Which statements do best describe Euchromatin or Heterochromatin? Please copy the word
behind the statement that best describes it.
is located at the periphery of nuclei.
is rich in repetitive DNA.
