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MCB4150 Exam 2 questions with complete solutions passed

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MCB4150 Exam 2 questions with complete solutions passed

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MCB 4150
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Uploaded on
September 22, 2025
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2025/2026
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MCB4150 Exam 2 questions with
complete solutions passed

largest and most metabolically diverse phylum of bacteria



-facultatively aerobic, microaerophilic, and strictly anaerobic



-6 Classes: Alpha, Beta, Gamma, Delta, Epsilon, and Zeta - correct answer ✔✔ Proteobacteria



energy dependent movement of electrons against a thermodynamic gradient



-Redox reactions reversible in this case



-PMF powers the reversion



-Used by chemolithoautotrophs to generate reducing power to fix CO2 - correct answer ✔✔
Reverse Electron Flow



-AMO is in the membrane and oxidizes NH3 to NH2OH/hydroxylamine



-HAO is in the periplasm and oxidizes NH2OH/hydroxylamine to NO2- - correct answer ✔✔
What do AMO and HAO do?



ammonia-oxidizing bacteria and archaea

, -key enzymes are ammonia monoxygenase (AMO) and hydroxylamine oxidoreductase (HAO) -
correct answer ✔✔ Nitrosifying Bacteria



nitrite-oxidizing bacteria



-key enzyme is nitrite oxidoreductase to oxidize nitrite to nitrate



-is what TRUE nitrifiers do



-no known archaea does oxidation of nitrite to nitrate



-end product of nitrification is terminal e- acceptor for denitrification



-Alpa, Beta, Gamma, and Epsilon proterobacteria - correct answer ✔✔ Nitrifying Bacteria



re responsible for the removal and leaching of nitrogen fertilizers from agricultural systems -
correct answer ✔✔ What do nitrifying/nitrosifying bacteria and archaea do?



Winogradsky was observing Beggiota, and saw when they were deprived of H2S, they lost
sulfur-granules



-hypothesized that the sulfur-granules were energy reserves of oxidized H2S, used when the
nutrient was not available



-when sulfur-granules disappeared, they oxidized to SO4 2-, a soluble form



-are chemolithoauotrophic sulfur-oxidizing bacteria - correct answer ✔✔ Discovery of
chemolithotrophic bacteria

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