MT 403 (Instrumental Analysis) Unit I questions with
verified answers
5 Deviations from Beer's Law Ans✓✓✓ High concentration, chemical
deviations, emission, instrumental deviations, mismatched cells
Absorbance Analysis of a Mixture Ans✓✓✓ The absorbance of a
solution at any set wavelength is the sum of all absorbances, Abs-tot =
Abs-1 + Abs-2 + Abs-3...Abs-n
Absorbance and transmittance Ans✓✓✓ A = -log(T), as A = 0 T = 100%,
and as A = infinite conc. T = 0
Absorption Ans✓✓✓ Energy from light transferred to matter
Absorption maxima Ans✓✓✓ Wavelength that has the highest
absorbance reading
Absorption spectroscopy Ans✓✓✓ UV-vis and atomic absorbtion
Accuracy Ans✓✓✓ Measure of conformity how the closeness of the
result
Acquisition Delay Ans✓✓✓ Irradiates sample inside phosphorimeters
,Amplitude Ans✓✓✓ Strength of wave
Analyte Interference Ans✓✓✓ Changes in signal magnitidue, chemical
reactions that occur in sample, volitization of analyte particles
Angle of Fluorescence Admissions Ans✓✓✓ 90 degrees
Applications of Fluorescence Spectrometry Ans✓✓✓ Drug detection,
biochemical analysis, compound discovery
Array dector Ans✓✓✓ Allows for fluorescence spectrum to be
obtained in the matter of seconds
Atomic Absorption Ans✓✓✓ Measurement of metallic elements and
their trace levels, elemental, optical, atomic mass
Atomic Absorption Spectroscopy Interferences Ans✓✓✓ Continuum
light interference, Zeeman Effect, Chemical Interference
Atomic Emission Spectrophotometer Ans✓✓✓ Similar to an AAS
spectrophotometer, but does not use a light source
Atomic Emission Spectroscopy Ans✓✓✓ Measures the emitted light
given off by atomic molecules
, Atomic Mass Spectroscopy Ans✓✓✓ Measures the mass to charge
ratio (M and M+1)
Atomic Radiation Ans✓✓✓ Radiation that passes through a
monoatomic particcle, E = E-elec
Band Pass FIlter Ans✓✓✓ Transmitted wavelenght = Cut off
wavelenght
Beam attenuator Ans✓✓✓ Cancels effect of source intensity
fluctuations and reduce optical power
Beer's Law Ans✓✓✓ A = elc
Beers Law Ans✓✓✓ A=ebc, A = absorbacne, e = molar coefficient, b =
tube length, c = concentration
Bioluminescence Ans✓✓✓ Light is emitted from living organisms,
naturally occurring, fireflies and jellyfish
Blackbody Radiation Ans✓✓✓ Emitts radiation at a constant
temperature (thermal equilibrium), continuum
verified answers
5 Deviations from Beer's Law Ans✓✓✓ High concentration, chemical
deviations, emission, instrumental deviations, mismatched cells
Absorbance Analysis of a Mixture Ans✓✓✓ The absorbance of a
solution at any set wavelength is the sum of all absorbances, Abs-tot =
Abs-1 + Abs-2 + Abs-3...Abs-n
Absorbance and transmittance Ans✓✓✓ A = -log(T), as A = 0 T = 100%,
and as A = infinite conc. T = 0
Absorption Ans✓✓✓ Energy from light transferred to matter
Absorption maxima Ans✓✓✓ Wavelength that has the highest
absorbance reading
Absorption spectroscopy Ans✓✓✓ UV-vis and atomic absorbtion
Accuracy Ans✓✓✓ Measure of conformity how the closeness of the
result
Acquisition Delay Ans✓✓✓ Irradiates sample inside phosphorimeters
,Amplitude Ans✓✓✓ Strength of wave
Analyte Interference Ans✓✓✓ Changes in signal magnitidue, chemical
reactions that occur in sample, volitization of analyte particles
Angle of Fluorescence Admissions Ans✓✓✓ 90 degrees
Applications of Fluorescence Spectrometry Ans✓✓✓ Drug detection,
biochemical analysis, compound discovery
Array dector Ans✓✓✓ Allows for fluorescence spectrum to be
obtained in the matter of seconds
Atomic Absorption Ans✓✓✓ Measurement of metallic elements and
their trace levels, elemental, optical, atomic mass
Atomic Absorption Spectroscopy Interferences Ans✓✓✓ Continuum
light interference, Zeeman Effect, Chemical Interference
Atomic Emission Spectrophotometer Ans✓✓✓ Similar to an AAS
spectrophotometer, but does not use a light source
Atomic Emission Spectroscopy Ans✓✓✓ Measures the emitted light
given off by atomic molecules
, Atomic Mass Spectroscopy Ans✓✓✓ Measures the mass to charge
ratio (M and M+1)
Atomic Radiation Ans✓✓✓ Radiation that passes through a
monoatomic particcle, E = E-elec
Band Pass FIlter Ans✓✓✓ Transmitted wavelenght = Cut off
wavelenght
Beam attenuator Ans✓✓✓ Cancels effect of source intensity
fluctuations and reduce optical power
Beer's Law Ans✓✓✓ A = elc
Beers Law Ans✓✓✓ A=ebc, A = absorbacne, e = molar coefficient, b =
tube length, c = concentration
Bioluminescence Ans✓✓✓ Light is emitted from living organisms,
naturally occurring, fireflies and jellyfish
Blackbody Radiation Ans✓✓✓ Emitts radiation at a constant
temperature (thermal equilibrium), continuum
