MCB 317 Exam 3 Questions with Correct Answers Already Passed
Gene 1 is normally off but is turned on in the presence of progesterone - Answers The hybrid version of
the gene containing the gene 1 coding region should be normally off but turned on in the presence of
progesterone
Gene 2 is constitutively expressed - Answers The hybrid version of the gene containing the gene 2
coding region is constitutively expressed
The consensus elements required for transcription of tRNA and 5sRNA genes by RNAPIII: - Answers are
found within the tRNA and 5sRNA coding regions
What is the general name for the type of DNA elements that allow eukaryotic genes to be turned on
under certain specific conditions, such as in the presence of glucocortoid hormones? - Answers
Response element
As discussed in lecture, virtually any enhancer can work in combination with virtually any promoter -
Answers True
Which eukaryotic control region typically mediates regulation of transcription? - Answers Enhancer
As discussed in lecture, linker scanner analysis defines the borders of a control region - Answers False
Transformation and transfection involve putting DNA molecules constructed in the lab into cells. What is
the difference between transformation and transfection? - Answers In transformation the DNA is stably
maintained and genes on transformed DNA can be expressed over long periods of time; whereas, in
transfection the DNA is not stably maintained and genes on transfected DNA are only transiently
expressed
Based on our discussion in lecture, which DNA control elements are required for activation of
transcription by eukaryotic RNAPII in vitro? - Answers Promoter
Based on our discussion in lecture, which DNA control elements are required for activation of
transcription by eukaryotic RNAPII in vivo? - Answers Promoter and enhancer
The promoter region for genes transcribed by which RNAP are contained entirely within the transcribed
region? - Answers RNAPIII
As discussed in lecture, once heterochromatin is formed it is copied/duplicated without having to be
assembled by spreading de novo each cell division - Answers True
As discussed in lecture, position effect variegation is a epigenetic phenomenon - Answers True
Linker scanner mutations are substitution mutations - Answers True
In terms of genes transcribed by RNAPII, no consensus element is found at all genes - Answers True
Gene 1 is normally off but is turned on in the presence of progesterone - Answers The hybrid version of
the gene containing the gene 1 coding region should be normally off but turned on in the presence of
progesterone
Gene 2 is constitutively expressed - Answers The hybrid version of the gene containing the gene 2
coding region is constitutively expressed
The consensus elements required for transcription of tRNA and 5sRNA genes by RNAPIII: - Answers are
found within the tRNA and 5sRNA coding regions
What is the general name for the type of DNA elements that allow eukaryotic genes to be turned on
under certain specific conditions, such as in the presence of glucocortoid hormones? - Answers
Response element
As discussed in lecture, virtually any enhancer can work in combination with virtually any promoter -
Answers True
Which eukaryotic control region typically mediates regulation of transcription? - Answers Enhancer
As discussed in lecture, linker scanner analysis defines the borders of a control region - Answers False
Transformation and transfection involve putting DNA molecules constructed in the lab into cells. What is
the difference between transformation and transfection? - Answers In transformation the DNA is stably
maintained and genes on transformed DNA can be expressed over long periods of time; whereas, in
transfection the DNA is not stably maintained and genes on transfected DNA are only transiently
expressed
Based on our discussion in lecture, which DNA control elements are required for activation of
transcription by eukaryotic RNAPII in vitro? - Answers Promoter
Based on our discussion in lecture, which DNA control elements are required for activation of
transcription by eukaryotic RNAPII in vivo? - Answers Promoter and enhancer
The promoter region for genes transcribed by which RNAP are contained entirely within the transcribed
region? - Answers RNAPIII
As discussed in lecture, once heterochromatin is formed it is copied/duplicated without having to be
assembled by spreading de novo each cell division - Answers True
As discussed in lecture, position effect variegation is a epigenetic phenomenon - Answers True
Linker scanner mutations are substitution mutations - Answers True
In terms of genes transcribed by RNAPII, no consensus element is found at all genes - Answers True
