UNE Medical Biochemistry Midterm
UPDATED Questions with CORRECT
Answers
Which of the following proteins is likely to have quaternary structure? - CORRECT
ANSWER - A multimeric protein that contains multiple peptide chains
Prosthetic groups are complex nonprotein molecules that participate in catalysis by providing
functional groups that form a covalent intermediate between the enzyme and the substrate.
Which of the following is an example of a coenzyme or cofactor that participates in a covalent
catalysis reaction? - CORRECT ANSWER - Pyridoxal phosphate
Which of the following best describes a protein domain? - CORRECT ANSWER -A
section of a protein structure sufficient to perform a particular chemical or physical task
Cleavage of fructose 1,6 bisphosphate to dihydroxyacetone and Glyceraldehyde 3-phosphate
involves the breaking of a carbon-carbon bond through a process other than oxidation or
hydrolysis. This is achieved by what class of enzymes? - CORRECT ANSWER - Lyase
A 55-year old man is brought to the emergency room with a chief complaint of chest pain and
tingling in his left arm. Elevation of which of the following enzymes in circulation would be
most indicative of myocardial infarction? - CORRECT ANSWER - Troponin
Allosteric activators of hemoglobin will increase substrate binding through which of the
following mechanisms? - CORRECT ANSWER - Binding the protein, and keeping it in
the "R" conformation
Which of the following best describes tertiary structure? - CORRECT ANSWER -
Interactions between alpha helices and beta sheets to form a domain
, How do most enzymes reduce the activation energy needed to move a reaction forward? -
CORRECT ANSWER - providing an active site most complementary to the transition state
Domains are common structural elements that retain a particular function within the protein.
Which of the following is an example of a protein domain? - CORRECT ANSWER - An
arrangement of beta-strands connected by alpha-helices (beta-alpha-beta-alpha)
Which amino acid would not be commonly found in the middle of an alpha-helical domain? -
CORRECT ANSWER - Proline
Which of the following is a true statement with regards to the malate-aspartate shuttle system? -
CORRECT ANSWER - Generates 2.5 molecules of ATP in mitochondria per each NADH
from glycolysis.
Complex II (Succinate Q-reductase) is not considered part of the process of oxidative
phosphorylation because it is unable to do which of the following activities? - CORRECT
ANSWER - Pump protons from the mitochondrial matrix into the inter membrane space.
Succinate is a substrate for which complex in the electron transport chain? - CORRECT
ANSWER - Complex II (Succinate-Q reductase)
The glycerophosphate shuttle is the major shuttle of most tissues. Which of the following best
describes the transfer of electrons by this shuttle? - CORRECT ANSWER - Electrons are
directly transferred from glycerol 3-phosphate to an FAD containing glycerol phosphate
dehydrogenase.
A 19-year old male is being evaluated for muscle weakness. Evaluation of a muscle biopsy
revealed normal rates of succinate oxidation but very low rates of pyruvate oxidation. Further
testing determined normal levels of enzymatic activity in both malate dehydrogenase and
pyruvate dehydrogenase. Based on this data, the patient may have a mutation in a gene encoding
for which mitochondrial protein? - CORRECT ANSWER - Complex I (NADH Q-
oxidoreductase)
UPDATED Questions with CORRECT
Answers
Which of the following proteins is likely to have quaternary structure? - CORRECT
ANSWER - A multimeric protein that contains multiple peptide chains
Prosthetic groups are complex nonprotein molecules that participate in catalysis by providing
functional groups that form a covalent intermediate between the enzyme and the substrate.
Which of the following is an example of a coenzyme or cofactor that participates in a covalent
catalysis reaction? - CORRECT ANSWER - Pyridoxal phosphate
Which of the following best describes a protein domain? - CORRECT ANSWER -A
section of a protein structure sufficient to perform a particular chemical or physical task
Cleavage of fructose 1,6 bisphosphate to dihydroxyacetone and Glyceraldehyde 3-phosphate
involves the breaking of a carbon-carbon bond through a process other than oxidation or
hydrolysis. This is achieved by what class of enzymes? - CORRECT ANSWER - Lyase
A 55-year old man is brought to the emergency room with a chief complaint of chest pain and
tingling in his left arm. Elevation of which of the following enzymes in circulation would be
most indicative of myocardial infarction? - CORRECT ANSWER - Troponin
Allosteric activators of hemoglobin will increase substrate binding through which of the
following mechanisms? - CORRECT ANSWER - Binding the protein, and keeping it in
the "R" conformation
Which of the following best describes tertiary structure? - CORRECT ANSWER -
Interactions between alpha helices and beta sheets to form a domain
, How do most enzymes reduce the activation energy needed to move a reaction forward? -
CORRECT ANSWER - providing an active site most complementary to the transition state
Domains are common structural elements that retain a particular function within the protein.
Which of the following is an example of a protein domain? - CORRECT ANSWER - An
arrangement of beta-strands connected by alpha-helices (beta-alpha-beta-alpha)
Which amino acid would not be commonly found in the middle of an alpha-helical domain? -
CORRECT ANSWER - Proline
Which of the following is a true statement with regards to the malate-aspartate shuttle system? -
CORRECT ANSWER - Generates 2.5 molecules of ATP in mitochondria per each NADH
from glycolysis.
Complex II (Succinate Q-reductase) is not considered part of the process of oxidative
phosphorylation because it is unable to do which of the following activities? - CORRECT
ANSWER - Pump protons from the mitochondrial matrix into the inter membrane space.
Succinate is a substrate for which complex in the electron transport chain? - CORRECT
ANSWER - Complex II (Succinate-Q reductase)
The glycerophosphate shuttle is the major shuttle of most tissues. Which of the following best
describes the transfer of electrons by this shuttle? - CORRECT ANSWER - Electrons are
directly transferred from glycerol 3-phosphate to an FAD containing glycerol phosphate
dehydrogenase.
A 19-year old male is being evaluated for muscle weakness. Evaluation of a muscle biopsy
revealed normal rates of succinate oxidation but very low rates of pyruvate oxidation. Further
testing determined normal levels of enzymatic activity in both malate dehydrogenase and
pyruvate dehydrogenase. Based on this data, the patient may have a mutation in a gene encoding
for which mitochondrial protein? - CORRECT ANSWER - Complex I (NADH Q-
oxidoreductase)
