Summary Advanced fluoresence: overview of all the techniques

Microscopy Types of microscopes
characteristics Transmitted light/bright field microscopy
(upright conformation)



Epi-illumination microscopy
(upright conformation)




Light sources
Mercury Arc lamp



Tungsten Halogen lamp

LEDs

Lasers

Detectors
Area detectors Charge-coupled device
(CCD)
Complementary metal
oxide semiconductor
(CMOS)
Point detectors Photomultiplier tube
(PMT)

, Point detectors Photomultiplier tube
(PMT)
Gallium arsenide
phosphide (GaAsP)
Avalanche photodiode

Zeiss Airy scan

Filters
Neutral density filters



Color filter




Dichromatic filter (dichroic mirror)




Microscopy Optical microscopy (transmitted light microscopy)
techniques Bright field microscopy




Dark field microsopy

,Dark field microsopy




Phase contrast microscopy




Polarization microscopy




Differential interference contrast (DIC)
microscopy




Fluorescence microscopy (background free signal detection)
Epi-fluorescence microscopy (widefield)




Confocal laser scannng microscopy (CLSM)

, Confocal laser scannng microscopy (CLSM)




Total internal reflection fluorescence
microscopy (TIRFM)




Two-Photon excitation microscopy (TPEM)
confocal



Spinning disk confocal microscopy (Nipkow
Disk)


Selective plane illumination microscopy
(SPIM)




Single molecule fluorescence (Single
particle tracking SPT)



Super Single molecule
resolution: localization microscopy
overcome (SMLM)
diffraction limit
(200-300 nm)