UConn MCB 2610 Final Lab Practical Exam Questions and Answers

UConn MCB 2610 Final Lab Practical Exam Questions and Answers The ________ method is a quick and easy way to isolate bacteria. - ANSWER-Streak plate How many times do you get culture from the tube while performing a t-streak? - ANSWER-Once Who do you contact to reschedule a lab? - ANSWER-Gino The culture tubes are disposed of in the gray trash bin. True/false? - ANSWER-FALSE What is the first thing you do after a spill? - ANSWER-Tell the TA Which of the following is an indirect mode of transmission - an insect, a cellphone, a sneeze? - ANSWER-Cellphone What is the last thing you do before leaving lab? - ANSWER-Wash hands for 20 seconds The outer cone is the hottest part of the flame. True/false? - ANSWER-FALSE Where are slides disposed of? - ANSWER-Sharps container What must you do to the loop before every use? - ANSWER-Heat in Bunsen-burner flame ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 2/11 True/False: When making a smear of the culture, you heat fix the slide before air drying it completely. - ANSWER-FALSE True/False: wiping down the bench with wescodyne is the last thing you do when leaving the lab. - ANSWER-FALSE What is the magnification value of the ONLY objective lens that should come in contact with immersion oil? - ANSWER-100x Pertaining to Gram stain, cells that retain crystal violet and appear purple are said to be_____. - ANSWER-Gram positive True/False: your name, the date, and sample information goes on the lid of the agar dish. - ANSWER- FALSE Pertaining to Gram stain, cells that do not retain crystal violet and appear pink, are said to be ____. - ANSWER-Gram negative True/False: heat used during staining causes sporulation. - ANSWER-FALSE True/False: malachite green stains the vegetative cells green, while the safranin stains endospores pink. - ANSWER-FALSE True/False: a vegetative cell is formed in response to certain environmental conditions such as depletion of nutrients. - ANSWER-TRUE ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 3/11 True/False: Gram stains and spore stains are examples of simple stains. - ANSWER-FALSE What causes bacterial endospore formation? - ANSWER-Response to environmental conditions, i.e., lack of nutrients What are the 4 phases of microbial growth? - ANSWER-Lag, exponential/log, stationary, death True/False: we will measure the optical density over time to determine growth rate. - ANSWER-TRUE Define generation time. - ANSWER-Time for population to double What does it mean for a culture to be in stationary phase? - ANSWER-Rate of replication matches rate of death True/False: you can use oil with the 40x objective lens. - ANSWER-FALSE True/False: generation time is calculated in the log phase. - ANSWER-TRUE What does it mean for a culture to be in log phase? - ANSWER-Rapid growth a