Bi
Folin-Lowry assay
Biochem Practical 2
, FOLIN-LOWRY ASSAY
Abstract
Protein assays are processes that allow us to quantify the concentrations of unknown proteins which
is vital as it is a pivotal part of the processes of protein engineering, the discovery of drugs and
biochemical analysis of compounds (Smith, 1985). Furthermore, protein assays are used in quality
control, research and they have clinical applications in that they allow us to monitor disease
progression hence plot out a better course of treatment (Wold, 1995). This experiment aimed to
quantify the concentrations of unknown samples of proteins using the standard curve obtained by
using the Follin-Lowry Protein Assay method. The process begins with a stock solution of Bovine
serum albumin being made up to the same standard of 1ml at varying concentrations. An Alkaline
copper color reagent is then add to all samples including the unknown samples. After a standing rest
period of 5 minutes the Folin-ciocalteu reagent is added and left to stand for 20 minutes, after which
the absorbances of all samples are taken using a spectrophotometer. Using the standard curve
obtained from the data collected the unknown concentration of the two samples are calculated. It
was found that the concentration of Sample A was 340 µg/ml and Sample B was found to be at a
concentration of 180 µg/ml. It can be concluded that Sample A has a much larger concentration than
Sample B.
Folin-Lowry assay
Biochem Practical 2
, FOLIN-LOWRY ASSAY
Abstract
Protein assays are processes that allow us to quantify the concentrations of unknown proteins which
is vital as it is a pivotal part of the processes of protein engineering, the discovery of drugs and
biochemical analysis of compounds (Smith, 1985). Furthermore, protein assays are used in quality
control, research and they have clinical applications in that they allow us to monitor disease
progression hence plot out a better course of treatment (Wold, 1995). This experiment aimed to
quantify the concentrations of unknown samples of proteins using the standard curve obtained by
using the Follin-Lowry Protein Assay method. The process begins with a stock solution of Bovine
serum albumin being made up to the same standard of 1ml at varying concentrations. An Alkaline
copper color reagent is then add to all samples including the unknown samples. After a standing rest
period of 5 minutes the Folin-ciocalteu reagent is added and left to stand for 20 minutes, after which
the absorbances of all samples are taken using a spectrophotometer. Using the standard curve
obtained from the data collected the unknown concentration of the two samples are calculated. It
was found that the concentration of Sample A was 340 µg/ml and Sample B was found to be at a
concentration of 180 µg/ml. It can be concluded that Sample A has a much larger concentration than
Sample B.
