MB (ASCP) Exam Questions & Answers
2023/2024
Predominant form of DNA - ANSWER-Right-handed B
DNA and histone interaction is due to what type of bond? - ANSWER-Ionic
What stains to the minor groove of DNA duplex? - ANSWER-SYBR Green
What is the most abundant type of RNA? - ANSWER-Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ANSWER-Genome and mRNA
What increases half-life of mRNA? - ANSWER-5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - ANSWER-Euchromatin
What is the most common type of eukaryotic DNA? - ANSWER-Satellite DNA
What specimen has the most DNA according to molecular weight? - ANSWER-White Blood Cells
What amino acids compose histones? - ANSWER-Lysine and Arginine
Amino acid that all proteins start with: - ANSWER-Methionine
What protects the DNA strand during replication? - ANSWER-Single-stranded binding protein
,Leading Strand - ANSWER-3' to 5' template
Lagging Strand - ANSWER-5' to 3' template
What DNA strand is being copied in transcription? - ANSWER-Antisense Strand
First step after newly formed mRNA is made? - ANSWER-Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? - ANSWER-
Telomerase
What polymerase has repair activity? - ANSWER-Beta/Epsilon
What polymerase acts on leading strand? - ANSWER-Delta
What polymerase acts on lagging strand? - ANSWER-Alpha
Which polymerase is used for mitochondrial replication and repair? - ANSWER-Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ANSWER-Topoisomerase
Which RNA polymerase is mainly for transcription? - ANSWER-RNA Pol II
Which polymerase is involved with mRNA synthesis? - ANSWER-RNA Pol II
What inactivates RNAses? - ANSWER-Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at
400C
What type of restriction enzyme recognizes palindromic sites? - ANSWER-Restriction Enzyme Type II
, What type of restriction enzyme lacks methylation activity? - ANSWER-Restriction Enzyme Type II
What is necessary to make cDNA? - ANSWER-Reverse transcriptase
Number of SNPs in a human genome? - ANSWER-11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - ANSWER-3 billion base pairs
The polymerization of acrylamide requires: - ANSWER-Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? - ANSWER-Inversely proportional
What is denatured during a southern blot? - ANSWER-Hydrogen bonds (inter-strand bonds)
Melting temperature formula - ANSWER-Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - ANSWER-5' G
Proper GC content of primer - ANSWER-35-65%
Inhibitors of PCR - ANSWER-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction: - ANSWER-Master mix > patient sample > positive control > negative
control
Pre Area - ANSWER-Positive Pressure
Post Area - ANSWER-Negative Pressure
2023/2024
Predominant form of DNA - ANSWER-Right-handed B
DNA and histone interaction is due to what type of bond? - ANSWER-Ionic
What stains to the minor groove of DNA duplex? - ANSWER-SYBR Green
What is the most abundant type of RNA? - ANSWER-Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ANSWER-Genome and mRNA
What increases half-life of mRNA? - ANSWER-5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - ANSWER-Euchromatin
What is the most common type of eukaryotic DNA? - ANSWER-Satellite DNA
What specimen has the most DNA according to molecular weight? - ANSWER-White Blood Cells
What amino acids compose histones? - ANSWER-Lysine and Arginine
Amino acid that all proteins start with: - ANSWER-Methionine
What protects the DNA strand during replication? - ANSWER-Single-stranded binding protein
,Leading Strand - ANSWER-3' to 5' template
Lagging Strand - ANSWER-5' to 3' template
What DNA strand is being copied in transcription? - ANSWER-Antisense Strand
First step after newly formed mRNA is made? - ANSWER-Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? - ANSWER-
Telomerase
What polymerase has repair activity? - ANSWER-Beta/Epsilon
What polymerase acts on leading strand? - ANSWER-Delta
What polymerase acts on lagging strand? - ANSWER-Alpha
Which polymerase is used for mitochondrial replication and repair? - ANSWER-Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ANSWER-Topoisomerase
Which RNA polymerase is mainly for transcription? - ANSWER-RNA Pol II
Which polymerase is involved with mRNA synthesis? - ANSWER-RNA Pol II
What inactivates RNAses? - ANSWER-Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at
400C
What type of restriction enzyme recognizes palindromic sites? - ANSWER-Restriction Enzyme Type II
, What type of restriction enzyme lacks methylation activity? - ANSWER-Restriction Enzyme Type II
What is necessary to make cDNA? - ANSWER-Reverse transcriptase
Number of SNPs in a human genome? - ANSWER-11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - ANSWER-3 billion base pairs
The polymerization of acrylamide requires: - ANSWER-Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration? - ANSWER-Inversely proportional
What is denatured during a southern blot? - ANSWER-Hydrogen bonds (inter-strand bonds)
Melting temperature formula - ANSWER-Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - ANSWER-5' G
Proper GC content of primer - ANSWER-35-65%
Inhibitors of PCR - ANSWER-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction: - ANSWER-Master mix > patient sample > positive control > negative
control
Pre Area - ANSWER-Positive Pressure
Post Area - ANSWER-Negative Pressure
