PAG 6 – Identification of the amino acids in a protein using paper chromatography
Results table:
Sample number Distance Distance Rf value Name of amino
travelled by travelled by acid
amino acid solvent front
(mm) (mm)
1 10 47 0.21 Arginine
2 19 47 0.40 Alanine
3 27 47 0.57 Methionine
4 38 47 0.81 Leucine
My Chromatogram:
Rf value calculation = 10/47 = 0.21
Results table:
Sample number Distance Distance Rf value Name of amino
travelled by travelled by acid
amino acid solvent front
(mm) (mm)
1 10 47 0.21 Arginine
2 19 47 0.40 Alanine
3 27 47 0.57 Methionine
4 38 47 0.81 Leucine
My Chromatogram:
Rf value calculation = 10/47 = 0.21
