Molecular Diagnostics Exam Questions with Correct Answers

in one nucleotide of DNA what are the 3 major moieties (components) that make it up? - Answer - 5 carbon sugar - phosphate group - nitrogen bases what is the overall charge of DNA and on which moiety is it found - Answer DNA is overall negative, it is found on the oxygen of phosphate backbone name a method that uses the fact that DNA is charged to get that method to work? - Answer Agarose gel electroporesis what types of bonds are formed during hybridization when a strand of DNA finds its complement - Answer hybridization is the formation of hydrogen bonds name 3 differences between DNA and RNA - Answer 1. on sugar, RNA has an OC on C-2' (ribose), and DNA has an H on C-2' (deoxyribose) 2. nitrogenous base: RNA uses Uracil in plase of thymine 3. RNA is typically single stranded and DNA is double dNTPs - Answer DNA building block NTPs - Answer RNA building block ddNTPs - Answer terminator for sequencing, not