BIOD 171 Microbiology Lab 3 Exam All correct Rated A+

List the four main steps used to prepare a WET mount, and indicate which (if any) step is optional. Your Answer:  Clean slide and make sure the slide has clean visible surface  Circle area on slide for easy location of specimen  Apply organism to slide If from culture, use sterile loop to spread on to slide If from plate, use sterile to pick colonies and mix with the drop of distilled water  View under microscope It is ideal to view motility of the organism Circle the area for easy location of specimen can be optional 1 – Clean slide 2 – Circle area on slide with wax/hydrophobic pen 3 – Apply organism to slide 4 – View under microscope *Note: there are no optional steps in the wet mount. Question 2 2.5 / 2.5 pts When preparing a glass slide for a dry mount, what cleaning solution was used? Your Answer: Ethanol 70% 70% ethanol Question 3 2.5 / 2.5 pts When performing a wet mount technique, what is the advantage of using a wax or hydrophobic pen? Your Answer: it does not let the water go out and it keeps it inside the ring Creating a hydrophobic barrier (the circle) helps to keep the water within the circle so it does not spill off of the slide. Question 4 2.5 / 2.5 pts What dye do Gram-negative bacteria primarily retain? Your Answer: Safranin Safranin